DNAJB6 (NM_005494) Human Untagged Clone

CAT#: SC110111

DNAJB6 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 6 (DNAJB6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNAJB6
• Synonyms: DJ4; DnaJ; HHDJ1; HSJ-2; HSJ2; LGMD1D; LGMD1E; LGMDD1; MRJ; MSJ-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110111 sequence for NM_005494 edited (data generated by NextGen Sequencing)
  ATGGTGGATTACTATGAAGTTCTAGGCGTGCAGAGACATGCCTCACCCGAGGATATTAAA
  AAGGCATATCGGAAACTGGCACTGAAGTGGCATCCAGATAAAAATCCTGAGAATAAAGAA
  GAAGCAGAGAGAAAATTCAAGCAAGTAGCGGAGGCATATGAAGTGCTGTCGGATGCTAAG
  AAACGGGACATCTATGACAAATATGGCAAAGAAGGATTAAATGGTGGAGGAGGAGGTGGA
  AGTCATTTTGACAGTCCATTTGAATTTGGCTTCACATTCCGTAACCCAGATGATGTCTTC
  AGGGAATTTTTTGGTGGAAGGGACCCATTTTCATTTGACTTCTTTGAAGACCCTTTTGAG
  GACTTCTTTGGGAATCGAAGGGGTCCCCGAGGAAGCAGAAGCCGAGGGACGGGGTCGTTT
  TTCTCTGCGTTCAGTGGATTTCCGTCTTTTGGAAGTGGATTTTCTTCTTTTGATACAGGA
  TTTACTTCATTTGGGTCACTAGGTCACGGGGGCCTCACTTCATTCTCTTCCACGTCATTT
  GGTGGTAGTGGCATGGGCAACTTCAAATCGATATCAACTTCAACTAAAATGGTTAATGGC
  AGAAAAATCACTACAAAGAGAATTGTCGAGAACGGTCAAGAAAGAGTAGAAGTTGAAGAA
  GATGGCCAGTTAAAGTCCTTAACAATAAATGGTAAGGAGCAGCTGCTGCGCTTGGATAAC
  AAGTAA
  
  Clone variation with respect to NM_005494.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005494 unedited
  TTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCTCGTGCCGAATTCG
  GCACGAGGAGGAGCCGCCGCCACCACCAGCGCAGCAGTCCTGGAGCTGTGAGGAGATTCG
  GGCCGTCACCCTGCCTCCCCTGCGTCCCGCCACCGGCCGCTTCTGTCCTCGGACCCATTC
  CAACAATCTCGTAAAACATGGTGGATTACTATGAAGTTCTAGGCGTGCAGAGACATGCCT
  CACCCGAGGATATTAAAAAGGCATATCGGAAACTGGCACTGAAGTGGCATCCAGATAAAA
  ATCCTGAGAATAAAGAAGAAGCAGAGAGAAAATTCAAGCAAGTAGCGGAGGCATATGAAG
  TGCTGTCGGATGCTAAGAAACGGGACATCTATGACAAATATGGCAAAGAAGGATTAAATG
  GTGGAGGAGGAGGTGGAAGTCATTTTGACAGTCCATTTGAATTTGGCTTCACATTCCGTA
  ACCCAGATGATGTCTTCAGGGAATTTTTTGGTGGAAGGGACCCATTTTCATTTGACTTCT
  TTGAAGACCCTTTTGAGGACTTCTTTGGGAATCGAAGGGGTCCCCGAGGAAGCAGAAGCC
  GAGGGAACGGGGTCGTTTTTCTCTGCGTTCAGTGGATTTCCGTCTTTTGGAAGTGGATTT
  TCTTCTTTTGATACAGGATTTACTTNCATTTGGGTCACTAGGTCACGGNNGGCCTCACTT
  CATTCTCTTNCACGTCATTTGGTGGGTAGTGGCATGGGGCAACTTCAAATCGATATCAAC
  TTCAACTAANATGGGTTAATGGCAGAAAAATACTACAAANAGAAATGTCGAGAACGGCAG
  AAAGAAATAAAAGTGAAAAAGATGGGCAGGTTAAGTCCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005494 unedited
  CCCCCTCCCCCCNCNCCCTTTCCCTCCTTCTCCCTTTTGATTTAGCACGCGGCCGCATAC
  TATGACCGGTTTTTTTTTTTTTTTTTTAAAGCAAATGACAAAGCCAGTTTACCAGCTTTA
  CTTTTTTAAACCTAACCTTAACATTACCTATTTAAACAATTGCCAAAACTTACTAAGTTG
  CCAGCATTCATGCCCAACTCGCAAAACATCCCTTAATTTATATTAAACCAGACATGTATT
  ACCATTAATGTATTAATATTTTTCACTACTAAATACTGAACAAAATTGAAATTATCCCTG
  TACAAGCAATCGACCTGGAAATGTTAACTTCACAGCAGGCCGACACTACGTGGCTCACAT
  TTTACACACAATGAAAAGCAGGCCCATGCTGGTGTTACCGTCCACACTCCGCCCTACACT
  AAAGAGCCCCGACTCACCTTGGAGTACGTCCAATAACAAGTCAAGCGCCTACAGAGATTT
  ACAATCACTTTATAGACAAAAAAACACCCGGTTTTATCATGCCGCCCCCACCATCCCCTC
  CACAGCCTATGACCATTAGAGCCTCCCACGACCTGCTTATAAATACCTCTTGGGCCCAAT
  TATACTGCAAGTCCAGTTCCCGTTGAAACCTTCCAAAAAATGTTCCCTGTCAACCCTTAC
  ATGTCGCCTGCCACCGCTTAAACATCCCCGCCAAGGGCGACCGTTCCACCACTCCCCACC
  CAACGGCACCACCTGCTCCTTCCCATCTATTGTCCAGGACTTAACCTGACCCATTTCTTT
  CCCCTATCAACTCTTTTTGGCCCGGCCCTCGATCATTTTTTTTGGCCAAGCATTTTCCGG
  CCCCTCAACCCTTCAAACTCCGATCCCCCATTGTTCTCCCAGCCCGCCCTGCGCTTTATC
  AACCAATTTACTCCGCACAACAATTAACTGTAGGCCCCTGTTCCCCCCACCCACCCAACC
  CGAACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005494
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005494.2, NP_005485.1
• RefSeq Size: 1568 bp
• RefSeq ORF: 726 bp
• Locus ID: 10049
• UniProt ID: O75190
• Cytogenetics: 7q36.3
• Domains: DnaJ
• Gene Summary: This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 3' coding region and has a distinct 3' UTR, compared to transcript variant 1. The resulting isoform (b) is shorter and distinct, compared to isoform a.