ARHGEF7 (NM_145735) Human Untagged Clone

SKU SC110008

ARHGEF7 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ARHGEF7
• Synonyms: BETA-PIX; COOL-1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145735, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAATTCCGCCGAGCAAACCGTTACGTGGCTCATCACTCTGGGGGTGCTGGAGTCGCCCAAAAAAACCA; TCTCGGACCCGGAGGGCTTTCTGCAGGCGTCGCTGAAGGATGGGGTGGTCCTCTGCAGGCTGCTGGAGCG; CCTGCTCCCCGGGACCATCGAGAAAGTCTACCCCGAGCCCCGGAGCGAGAGCGAGTGCCTGAGCAACATC; CGCGAGTTCCTGCGCGGCTGCGGGGCTTCCCTGCGGCTGGAGACGTTTGATGCAAATGATTTGTATCAGG; GGCAGAATTTTAACAAGGTCCTCAGTTCCTTAGTGACTCTAAATAAAGTAACAGCAGACATCGGGCTGGG; GAGTGACTCCGTGTGTGCCCGGCCCTCGTCTCACCGCATAAAGTCTTTTGACTCCCTTGGATCACAGTCT; TTGCACACTCGGACTTCAAAACTGTTCCAGGGCCAGTATCGGAGTTTGGACATGACCGATAATAGCAACA; ATCAACTGGTAGTAAGAGCAAAGTTTAACTTCCAGCAGACCAATGAGGACGAGCTTTCCTTCTCAAAAGG; AGACGTCATCCATGTCACCCGTGTGGAAGAGGGAGGCTGGTGGGAGGGCACACTCAACGGCCGGACCGGC; TGGTTCCCCAGCAACTACGTGCGCGAGGTCAAGGCCAGCGAGAAGCCTGTGTCTCCCAAATCAGGAACAC; TGAAGAGCCCTCCCAAAGGATTTGATACGACTGCCATAAACAAAAGCTATTACAATGTGGTGCTACAGAA; TATTTTAGAAACAGAAAATGAATATTCTAAAGAACTTCAGACTGTGCTTTCAACGTACCTACGGCCATTG; CAGACCAGTGAGAAGTTAAGTTCAGCAAACATTTCATATTTAATGGGAAATCTAGAAGAAATATGTTCTT; TCCAGCAAATGCTCGTACAGTCTTTAGAAGAATGCACCAAGTTGCCCGAAGCTCAGCAGAGAGTCGGAGG; CTGCTTTTTAAACCTGATGCCACAGATGAAAACCCTGTACCTCACGTATTGTGCCAATCACCCTTCTGCA; GTGAATGTCCTCACGGAACACAGTGAGGAGTTGGGGGAGTTCATGGAGACCAAAGGTGCCAGCAGCCCTG; GGATTCTCGTGCTGACCACGGGCCTGAGCAAACCCTTCATGCGCCTGGATAAATACCCTACGCTGCTCAA; AGAGCTCGAGAGACACATGGAGGATTATCATACAGATAGACAAGATATTCAAAAATCCATGGCTGCCTTC; AAAAACCTTTCAGCCCAATGTCAAGAAGTCCGGAAGAGGAAAGAGCTTGAGCTGCAGATCCTGACGGAAG; CCATCCGGAACTGGGAGGGCGATGACATTAAAACTCTGGGCAACGTCACTTACATGTCCCAGGTCCTGAT; TCAGTGTGCCGGAAGTGAGGAAAAGAATGAAAGATATCTTCTACTCTTCCCAAATGTTTTGCTAATGTTG; TCTGCCAGTCCTAGGATGAGTGGCTTTATCTATCAGGGAAAGCTTCCAACGACAGGAATGACAATCACAA; AGCTTGAGGACAGTGAAAATCATAGAAATGCATTTGAAATATCAGGGAGCATGATTGAGCGGATATTAGT; GTCGTGCAACAACCAGCAGGATCTGCAGGAATGGGTGGAGCACCTACAGAAGCAAACGAAGGTCACGTCT; GTGGGAAACCCCACCATAAAGCCTCATTCAGTGCCATCTCATACCCTCCCCTCCCACCCGGTCACTCCGT; CCAGCAAGCACGCAGACAGCAAGCCCGCGCCGCTGACGCCCGCCTACCACACGCTGCCCCACCCCTCCCA; CCACGGCACCCCGCACACCACCATCAACTGGGGACCCCTGGAGCCTCCGAAAACACCCAAGCCCTGGAGC; CTGAGCTGCCTGCGGCCCGCGCCTCCCCTCCGGCCCTCAGCTGCTCTCTGCTACAAGGAGGATCTTAGTA; AGAGCCCTAAGACCATGAAAAAGCTGCTGCCCAAGCGCAAACCTGAACGGAAGCCTTCAGATGAGGAGTT; CGCGTCCCGGAAAAGCACAGCTGCTTTGGAAGAAGATGCTCAGATTCTGAAAGTCATTGAAGCTTACTGC; ACCAGCGCCAAAACAAGGCAAACACTCAATTCAACATGGCAAGGCACTGACCTGATGCATAATCACGTCT; TGGCTGATGATGACCAACCAAGCCTAGACTCCCTGGGGCGTCGCAGTAGCCTTTCTCGTTTGGAGCCTTC; AGACCTCTCGGAAGACTCTGACTATGACAGTATATGGACAGCCCATAGTTACAGAATGGGTTCTACATCT; CGTAAGAGCTGTTGCTCATATATCTCTCACCAGAACTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145735 unedited; TACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGNAGCCGCTCCTGAGAAGCGCCTG; ACAGCGGGCCGGGGCGCACGGAGAAGCGGGCCGGGCCGGACCTGCTGGGCCGCGCCGAGC; CAATCGCCGGCGCCGGCCGCTCGATGGGCGAGGCGGCGGCGGCGGCGGCGGGGGCCGCGG; GCCGGGCCGCCGCTCCGAGGTGAAGGCGCGCGCCCCTCCCCGCCTGCCTCCCGGGCCGCA; GCGATGAATTCCGCCGAGCAAACCGTTACGTGGCTCATCACTCTGGGGGTGCTGGAGTCG; CCCAAAAAAACCATCTCGGACCCGGAGGGCTTTCTGCAGGCGTCGCTGAAGGATGGGGTG; GTCCTCTGCAGGCTGCTGGAGCGCCTGCTCCCCGGGACCATCGAGAAAGTCTACCCCGAG; CCCCGGAGCGAGAGCGAGTGCCTGAGCAACATCCGCGAGTTCCTGCGCGGCTGCGGGGCT; TCCCTGCGGCTGGAGACGTTTGATGCAAATGATTTGTATCAGGGGCAGAATTTTAACAAG; GTCCTCAGTTCCTTAGTGACTCTAAATAAAGTAACAGCAGACATCGGGCTGGGGAGTGAC; TCCGTGTGTGCCCGGCCCTCGTCTCACCGCATAAAGTCTTTTGACTCCCTTGGATCACAG; TCTTTGCACACTCGGACTTCAAAACTGTTCCAGGGCCAGTATCGGNAGTTTGGACATGAC; CGANNTATAGCACAATCAACTGGTAGTAAGAGCCAAAGTTAACTTCCAGCAGACCAAGGG; AGACGAGCNTTTCCTTCTCAAAAGGAGACGTCATCCATGTCACCCGTGTTGAANAAGGNA; GCTGGTTGGNAGGCACACTCACGGGNCGGACCGGGTGGTTCCCCNCAACTACGTGCGCAA; GGTCAGGCCANCGAAAAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145735 unedited; CAGCTATGGACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAATAAC; GAGCCCATGGCAACCTAGTAATTCTTCAATGAACTTCAATATCCCATTACAATGAGGCAT; TGCGGTTTTATACTATGGGGTTGCTGTAAATGGAGTTTCACTGCAGCTGGTTGCTGAAAA; TAATGATGTGTCCATGGCTCTTCCAGACAACATAAGTATGGATGTCAGGGGTGGGGAGCC; GCTGGATCTGAAGCCACAGCACTTGACTTTGTAAGTACTCCAAATGCCTCTACTACAGCC; CAGTAGTCTACACTTGCAAAGGCTGAATGCTTTATCTTTCGGGCTTTAAACTGTTAATGT; ATACATCTTACTGTAGACCCTTACAGGATCGCTCTGTGCCTCATCCCAGAATTCACTTGC; CTTTCATGCCATTTCGGATTGCCTACCCCCGCCCACCCCCGACCCCTCGTACACCAGCCC; TGACTTTCGAAAGCACACCTTACCTCTCCCCGGCCACCCCCCAACACTCTCACCACCTCC; CCCCCGTAAGGTCCACGCTAGTTGGTTACAAACCCTCACGTACCCCCTCCCGCCCCACTG; CTCCAGCTTCGCCCCCACCCCCGCTCCCCCCCCCCCCCGGCACAAGCGCGTGCATTTGGC; TTTCTCACAATCCCGCCCGCCGTCTCCCTCCCCCCCCCTCCCCCCTCCCCCATATCTCCG; TTTCCCCACCTCTACCACATCAGTTCCCATTTCTCTATCGCCCCCGCCGCCCATCAACAC; ACCCCCCTTCTCCACCCTCCCCTTTCGAGGCGACCAAAACAATACTTAAAACGCCCCCTC; CCCCCGCCCCTCTTCCCCACTTCTCATCCGCCAAGGTCGCCACCCCCTCCCCCCTCTCCC; CTCATACCCCCCTCCCCCTCCCGCCCCCCCCATATTCCCCTTCTTGCCCTCACCCACTCT; CGTACACGCCCGCCTCCGCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_145735
• Insert Size: 3890 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145735.1, NP_663788.1
• RefSeq Size: 5304 bp
• RefSeq ORF: 2349 bp
• Locus ID: 8874
• UniProt ID: Q14155
• Cytogenetics: 13q34
• Domains: CH, PH, RhoGEF, SH3
• Protein Pathways: Regulation of actin cytoskeleton
• Summary: This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016]; Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 3. The resulting protein (isoform b) is shorter than isoform c.