TBXAS1 (NM_001061) Human Untagged Clone

CAT#: SC109823

TBXAS1 (untagged)-Human thromboxane A synthase 1 (platelet) (TBXAS1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBXAS1
• Synonyms: BDPLT14; CYP5; CYP5A1; GHOSAL; THAS; TS; TXAS; TXS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109823 sequence for NM_001061 edited (data generated by NextGen Sequencing)
  ATGATGGAAGCCTTGGGGTTTCTAAAATTGGAAGTGAATGGCCCCATGGTGACGGTGGCC
  CTGTCAGTGGCTCTCTTGGCCCTCCTGAAATGGTACTCCACATCAGCATTCTCAAGACTG
  GAGAAGTTAGGCCTCAGACATCCCAAGCCTTCTCCTTTCATTGGAAACTTGACATTTTTC
  CGCCAGGGTTTTTGGGAAAGCCAAATGGAGCTCAGAAAGCTGTATGGACCTCTGTGTGGG
  TACTATCTTGGTCGTCGGATGTTTATTGTTATTTCTGAGCCAGACATGATCAAGCAGGTG
  TTGGTTGAGAACTTCAGTAACTTTACCAACAGAATGGCGTCGGGTTTGGAGTTCAAGTCG
  GTAGCCGACAGCGTTCTGTTTTTACGTGACAAAAGATGGGAAGAGGTCAGAGGTGCCCTG
  ATGTCTGCTTTCAGTCCTGAAAAGCTGAACGAGATGGTTCCCCTCATCAGCCAAGCCTGC
  GACCTTCTCCTGGCTCATTTAAAACGCTATGCGGAATCTGGGGACGCATTTGACATCCAG
  AGGTGCTACTGCAATTACACCACAGATGTGGTTGCCAGCGTCGCCTTTGGCACCCCGGTG
  GACTCCTGGCAGGCCCCTGAGGATCCCTTTGTGAAACACTGCAAGCGTTTCTTCGAATTC
  TGCATCCCCAGACCTATCCTGGTTTTACTCTTATCATTTCCATCCATAATGGTCCCACTG
  GCCCGGATTTTGCCCAATAAGAACCGAGACGAACTGAATGGCTTTTTTAACAAACTCATT
  AGGAATGTGATTGCCTTGCGGGACCAGCAAGCTGCCGAAGAGAGGCGGAGAGACTTCCTC
  CAAATGGTCCTGGATGCCCGACATTCTGCAAGTCCCATGGGCGTGCAAGACTTTGACATC
  GTCAGAGACGTTTTCTCCTCTACTGGGTGCAAGCCGAACCCTTCCCGGCAACACCAGCCC
  AGCCCTATGGCCAGGCCTTTGACTGTGGATGAGATTGTGGGCCAGGCCTTCATCTTCCTC
  ATCGCTGGCTATGAAATCATCACCAACACACTTTCTTTTGCCACCTACCTACTGGCCACC
  AACCCTGACTGCCAAGAGAAGCTTCTGAGAGAGGTAGACGTTTTTAAGGAGAAACACATG
  GCCCCTGAGTTCTGCAGCCTCGAGGAAGGCCTGCCCTATCTGGACATGGTGATTGCAGAG
  ACGCTGAGGATGTACCCGCCAGCTTTCAGATTCACACGGGAGGCAGCTCAGGACTGCGAG
  GTGCTGGGGCAGCGCATCCCCGCAGGCGCTGTGCTAGAGATGGCCGTGGGTGCCCTGCAC
  CATGACCCTGAGCACTGGCCAAGCCCGGAGACCTTCAACCCTGAAAGGTTCACGGCTGAG
  GCCCGGCAGCAGCACCGGCCCTTCACGTACCTGCCCTTCGGGGCCGGCCCACGGAGCTGC
  CTCGGGGTGCGTCTAGGGCTGCTTGAGGTCAAGTTGACACTGCTCCACGTGCTGCACAAG
  TTCCGGTTCCAAGCCTGCCCTGAGACCCAGGTACCGCTGCAGCTAGAATCCAAATCTGCC
  CTAGGTCCAAAAAATGGTGTCTATATCAAGATCGTATCCCGCTGA
  
  Clone variation with respect to NM_001061.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001061 unedited
  TATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGCCTCGTGCCGAATTCGGCACGA
  GGGTGATGTTTGCTTGGTTGCCTGTTCCCTTTTCTACCTGCAGAGCACGGTTCCCATAAG
  GGCGGCGAGATCAGCCTCCTGTCTCATCTGGAAGACCACCACTCTGGGGTCTCAGAGGAA
  TGATGGAAGCCTTGGGGTTTCTAAAATTGGAAGTGAATGGCCCCATGGTGACGGTGGCCC
  TGTCAGTGGCTCTCTTGGCCCTCCTGAAATGGTACTCCACATCAGCATTCTCAAGACTGG
  AGAAGTTAGGCCTCAGACATCCCAAGCCTTCTCCTTTCATTGGAAACTTGACATTTTTCC
  GCCAGGGTTTTTGGGAAAGCCAAATGGAGCTCAGAAAGCTGTATGGACCTCTGTGTGGGT
  ACTATCTTGGTCGTCGGATGTTTATTGTTATTTCTGAGCCAGACATGATCAAGCAGGTGT
  TGGTTGAGAACTTCAGTAACTTTACCAACAGAATGGCGTCGGGTTTGGAGTTCAAGTCGG
  TAGCCGACAGCGTTCTGTTTTTACGTGACAAAAGATGGGAAGAGGTCAGAGGTGCCCTGA
  TGTCTGCTTTCAGTCCTGAAAAGCTGAACGAGATGGTTCCCCTCATCAGCCAAGCCTGCG
  ACCTTCTCCTGGCTCATTTAAAACGCTATGCGGAATCTGGGGACGCATTTGACTCCAGAG
  GTGCTACTGCAATTACCCACAGATGTGGTTGCCAGCGTCGCCTTGGCACCCCGTGGACTC
  CTGGNCAGCCCTTGAGATCCCTTTTGTGAACACTGCAGCGTTTCTTCGAATCTGCATCCC
  AGACTATNCNGGNTTTACTCTTATCATTTCTCCATATGGNCCACTGCCCCNGATTTTGCC
  AATAGACCGAGAGACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001061 unedited
  GCAACTTTCCAGGGCCAGGAAGNAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGNTTTTTTTTTTTTTT
  TTTTTTTTTCCAAGGGCAACAAACGTTTATTAAGCATTTAGGAAATGTTATGTAAAGAAC
  CTTTTATCCTTGCATGCCAGGCACTCTTTCAATCACTTCAGGGACATTTTTCCAAAATTC
  TGAACATCCACACTTAGGGTTTTCTTTGAATTTGGGGGGGCCCTCCCCCCACCCGGCAGC
  CTTTTGTGTCAGCGGGATACGATCTTGATATAAACACCATTTTTTGGACCTAGGGCAGAT
  TTGGATTCTAGCTGCAGCGGTACCTGGGTCTCAGGGCAGGCTTGGAACCGGAACTTGTGG
  NNNACGTGGAGCAGTGTCAACTTGACCTCAAGCAGCCCTAAACGCACCCCGAGGCAGCTC
  CGTGGGCCGGCCCCGAAGGGCAGGTACGTGAAGGGCCGGTGCTGCTGCCGGGCNCTCACC
  GTGAACCTTTCAGGGTTGAAGGTCTCCGGGCTTGGCCAGTGCTCAGGGTCATGGGGCAGG
  GCACCCACGGCCATCTCTAGCACANCGCCTGCGGGGATGCGCTTGCCCAGCACCTCGCAG
  TCCTGAGCTGCCTCCCGTGGGAATTGAAAGCTGGCGGGACATCCTCAGCGGTTCTGCAAT
  CACCATGTCCACAAAGNNNNACCCTCCTCGAGGCTGCAAACTCAGGGGCCATGGGTTTCT
  CTTAAAAACGTCTACCTCTTTCAAAACTTTTCTTGGCAATAAGGGTTGGGTGGCACGTAG
  GTAGTGGCAAAGAAAGAGGTGTGGGTGAAGATCTATTACCCACGATAGGAAAATGAAGGC
  TTGCCCACATTTCTTCCAGGCAAGGGCGGCATAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001061
• Insert Size: 1960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001061.2, NP_001052.1
• RefSeq Size: 1946 bp
• RefSeq ORF: 1605 bp
• Locus ID: 6916
• UniProt ID: P24557
• Cytogenetics: 7q34
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. The enzyme plays a role in several pathophysiological processes including hemostasis, cardiovascular disease, and stroke. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
  Transcript Variant: This variant (1, also known as TXS-I) encodes isoform 1, which is also known as isoform TXS-I. Both variants 1 and 3 encode the same isoform. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is better supported by available conservation data and peptide data. This CCDS represents use of a downstream start codon, shortening the protein by a single amino acid.