SNAP25 (NM_130811) Human Untagged Clone

SKU SC109782

SNAP25 (untagged)-Human synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: SNAP25
• Synonyms: bA416N4.2; CMS18; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SUP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_130811 edited; GAATTCGGCACGAGGGAACACAACCCTCCCGAGAAGCCCAGGTCCAGAGCCAAACCCGTC; ACTGACCCCCCAGCCCAGGCGCCCAGCCACTCCCCACCGCTACCATGGCCGAAGACGCAG; ACATGCGCAATGAGCTGGAGGAGATGCAGCGAAGGGCTGACCAGTTGGCTGATGAGTCGC; TGGAAAGCACCCGTCGTATGCTGCAACTGGTTGAAGAGAGTAAAGATGCTGGTATCAGGA; CTTTGGTTATGTTGGATGAACAAGGAGAACAACTCGATCGTGTCGAAGAAGGCATGAACC; ATATCAACCAAGACATGAAGGAGGCTGAGAAAAATTTAAAAGATTTAGGGAAATGCTGTG; GCCTTTTCATATGTCCTTGTAACAAGCTTAAATCAAGTGATGCTTACAAAAAAGCCTGGG; GCAATAATCAGGACGGAGTGGTGGCCAGCCAGCCTGCTCGTGTAGTGGACGAACGGGAGC; AGATGGCCATCAGTGGCGGCTTCATCCGCAGGGTAACAAATGATGCCCGAGAAAATGAAA; TGGATGAAAACCTAGAGCAGGTGAGCGGCATCATCGGGAACCTCCGTCACATGGCCCTGG; ATATGGGCAATGAGATCGATACACAGAATCGCCAGATCGACAGGATCATGGAGAAGGCTG; ATTCCAACAAAACCAGAATTGATGAGGCCAACCAACGTGCAACAAAGATGCTGGGAAGTG; GTTAAGTGTGCCCACCCGTGTTCTCCTCCAAATGCTGTCGGGCAAGATAGCTCCTTCATG; CTTTTCTCATGGTATTATCTAGTAGGTCTGCACACATAACACACATCAGTCCACCCCCAT; TGTGAATGTTGTCCTGTGTCATCTGTCAGCTTCCCAACAATACTTTGTGTCTTTTGTTCT; CTCTTGGTCTCTTTCTTTCCAAAGGTTGTACATAGTGGTCATTTGGTGGCTCTAACTCCT; TGATGTCCTGAGTTTCATTTTTCATTTTCTCTCCTCGGTGGCATTTGCTGAATAACAACA; ATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCACAGTATTGTTCTTGTAAAAC; TGTGACATTCCACAGAGTTACTGCCACGGTCCTTTGAGTGTCAGGCTCTGAATCTCTCAA; AATGTGCCGTCTTTGGTTCCTCATGGCTGTTATCTGTCTTTATGATTTCATGATTAGACA; ATGTGGAATTACATAACAGGCATTGCACTAAAAGTGATGTGATTTATGCATTTATGCATG; AGAACTAAATAGATTTTTAGATTCCTACTTAAACAAAAACTTTCCATGACAGTAGCATAC; TGATGAGACAACACACACACACACAAAACAACAGCAACAACAACAGAACAACAACAAAGC; ATGCTCAGTATTGAGACACTGTCAAGATTAAGTTATACCAGCAAAAGTGCAGTAGTGTCA; CTTTTTTCCTGTCAATATATAGAGACTTCTAAATCATAATCATCCTTTTTTAAAAAAAAG; AATTTTAAAAAAGATGGATTTGACACACTCACCATTTAATCATTTCCAGCAAAATATATG; TTTGGCTGAAATTATGTCAAATGGATGTAATATAGGGTTTGTTTGCTGCTTTTGATGGCT; ACGTTTTGGAGAGAGCAATCTTGCTGTGAAACAGTGTGGATGTAAATTTTATAAGGCTGA; CTCTTACTAACCACCATTTCCCCTGTGGTTTGTTATCAGTACAATTCTTTGTTGCTTAAT; CTAGAGCTATGCACACCAAATTGCTGAGATGTTTAGTAGCTGATAAAGAAACCTTTTAAA; AAAATAATATAAATGAATGAAATATAAACTGTGAGATAAATATCATTATAGCATGTAATA; TTAAATTCCTCCTGTCTCCTCTGTCAGTTTGTGAAGTGATTGACATTTTGTAGCTAGTTT; AAAATTATTAAAAATTATAGACTCCAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130811 unedited; TTCGGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCCGAGGGAACACAAC; CCTCCCGAGAAGCCCAGGTCCAGAGCCAAACCCGTCACTGACCCCCCAGCCCAGGCGCCC; AGCCACTCCCCACCGCTACCATGGCCGAAGACGCAGACATGCGCAATGAGCTGGAGGAGA; TGCAGCGAAGGGCTGACCAGTTGGCTGATGAGTCGCTGGAAAGCACCCGTCGTATGCTGC; AACTGGTTGAAGAGAGTAAAGATGCTGGTATCAGGACTTTGGTTATGTTGGATGAACAAG; GAGAACAACTCGATCGTGTCGAAGAAGGCATGAACCATATCAACCAAGACATGAAGGAGG; CTGAGAAAAATTTAAAAGATTTAGGGAAATGCTGTGGCCTTTTCATATGTCCTTGTAACA; AGCTTAAATCAAGTGATGCTTACAAAAAAGCCTGGGGCAATAATCAGGACGGAGTGGTGG; CCAGCCAGCCTGCTCGTGTAGTGGACGAACGGGAGCAGATGGCCATCAGTGGCGGCTTCA; TCCGCAGGGTAACAAATGATGCCCGAGAAAATGAAATGGATGAAAACCTAGAGCAGGTGA; GCGGCATCATCGGGAACCTCCGTCACATGGCCCTGGATATGGGCAATGAGATCGATACAC; AGAATCGCCAGATCGACAGGATCATGGAGAAGGCTGATTCCAACAAAACCAGAATTGATG; AGGCCAACCAACGTGCAACANAGATGCTGGGAAGCGGCTAAGTGTGCCCACCCCGTGTTC; TCCTCCAAATGCCTGTCGGCAGATAGCTCCTTCATGCCTTTCCTCATGGATTATCTAGNA; GGGTCTGCCACATAACACCACATAGTCCCACCCCATTGTGAAAGTTGTCCTGTGTCATCC; GTCAGCTTTCCACCATACTTGTGGGCTTTTGTTCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_130811 unedited; GCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGGAGTCTATAATTTTTAATAATT; TTAAACTAGCTACAAAATGTCAATCACTTCACAAACTGACAGAGGAGACAGGAGGAATTT; AATATTACATGCTATAATGATATTTATCTCACAGTTTATATTTCATTCATTTATATTATT; TTTTTAAAAGGTTTCTTTATCAGCTACTAAACATCTCAGCAATTTGGTGTGCATAGCTCT; AGATTAAGCAACAAAGAATTGTACTGATAACAAACCACAGGGGAAATGGTGGTTAGTAAG; AGTCAGCCTTATAAAATTTACATCCACACTGTTTCACAGCAAGATTGCTCTCTCCAAAAC; GTAGCCATCAAAAGCAGCAAACAAACCCTATATTACATCCATTTGACATAATTTCAGCCA; AACATATATTTTGCTGGAAATGATTAAATGGTGAGTGTGTCAAATCCATCTTTTTTAAAA; TTCTTTTTTTTAAAAAAGGATGATTATGATTTAGAAGTCTCTATATATTGACAGGAAAAA; AGTGACACTACTGCACTTTTGCTGGTATAACTTAATCTTGACAGTGTCTCAATACTGAGC; ATGCTTTGTTGNTGNTCTGTTGTTGTTGCTGTTGNTTTGTGTGTGTGTGTGTTGGCTCAT; CAGTATGCTACTGTCATGGAAAGTTTTTGTTAAGTANGAATCTAAAATCTATTTAGCTCT; CATGCATAAATGCATAAATCACATCACTTTTTAGTGCATGCCTGTTATGTATTNCCCATT; GCCTATCCATGAATCCTAAGACGGTAACAGCCCTGAGGAACCAAGACGCCCATTTTGGAG; ATTCAGACCTGACCTCAAAGACCGTGCAGTACCCTGTGAATGTACAGTTTACAGACCATA; CGTGGATGAAAGATCACACCATGAGCATCCTATTGTGTATTCCCAAGCCCCGNGAAAAAG; AAATGACCTCGCTCAGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_130811
• Insert Size: 1960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_130811.1, NP_570824.1
• RefSeq Size: 2053 bp
• RefSeq ORF: 621 bp
• Locus ID: 6616
• UniProt ID: P60880
• Cytogenetics: 20p12.2
• Domains: SNAP-25, t_SNARE
• Protein Families: Druggable Genome
• Protein Pathways: SNARE interactions in vesicular transport
• Summary: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) contains an alternate exon 5 as compared to transcript variant 1. This results in an isoform (SNAP25B) that is the same length, and contains the same N- and C-termini as isoform SNAP25A, but has 9 aa differences internally.