CDw75 (ST6GAL1) (NM_003032) Human Untagged Clone

SKU SC109756

ST6GAL1 (untagged)-Human ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: CDw75
• Synonyms: SIAT1; ST6GalI; ST6N
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003032, the custom clone sequence may differ by one or more nucleotides; ; ; ATGATTCACACCAACCTGAAGAAAAAGTTCAGCTGCTGCGTCCTGGTCTTTCTTCTGTTTGCAGTCATCT; GTGTGTGGAAGGAAAAGAAGAAAGGGAGTTACTATGATTCCTTTAAATTGCAAACCAAGGAATTCCAGGT; GTTAAAGAGTCTGGGGAAATTGGCCATGGGGTCTGATTCCCAGTCTGTATCCTCAAGCAGCACCCAGGAC; CCCCACAGGGGCCGCCAGACCCTCGGCAGTCTCAGAGGCCTAGCCAAGGCCAAACCAGAGGCCTCCTTCC; AGGTGTGGAACAAGGACAGCTCTTCCAAAAACCTTATCCCTAGGCTGCAAAAGATCTGGAAGAATTACCT; AAGCATGAACAAGTACAAAGTGTCCTACAAGGGGCCAGGACCAGGCATCAAGTTCAGTGCAGAGGCCCTG; CGCTGCCACCTCCGGGACCATGTGAATGTATCCATGGTAGAGGTCACAGATTTTCCCTTCAATACCTCTG; AATGGGAGGGTTATCTGCCCAAGGAGAGCATTAGGACCAAGGCTGGGCCTTGGGGCAGGTGTGCTGTTGT; GTCGTCAGCGGGATCTCTGAAGTCCTCCCAACTAGGCAGAGAAATCGATGATCATGACGCAGTCCTGAGG; TTTAATGGGGCACCCACAGCCAACTTCCAACAAGATGTGGGCACAAAAACTACCATTCGCCTGATGAACT; CTCAGTTGGTTACCACAGAGAAGCGCTTCCTCAAAGACAGTTTGTACAATGAAGGAATCCTAATTGTATG; GGACCCATCTGTATACCACTCAGATATCCCAAAGTGGTACCAGAATCCGGATTATAATTTCTTTAACAAC; TACAAGACTTATCGTAAGCTGCACCCCAATCAGCCCTTTTACATCCTCAAGCCCCAGATGCCTTGGGAGC; TATGGGACATTCTTCAAGAAATCTCCCCAGAAGAGATTCAGCCAAACCCCCCATCCTCTGGGATGCTTGG; TATCATCATCATGATGACGCTGTGTGACCAGGTGGATATTTATGAGTTCCTCCCATCCAAGCGCAAGACT; GACGTGTGCTACTACTACCAGAAGTTCTTCGATAGTGCCTGCACGATGGGTGCCTACCACCCGCTGCTCT; ATGAGAAGAATTTGGTGAAGCATCTCAACCAGGGCACAGATGAGGACATCTACCTGCTTGGAAAAGCCAC; ACTGCCTGGCTTCCGGACCATTCACTGCTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003032 unedited; CCCCCATTTCCCCCCCGTTGCCGCTTTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT; AAGCAGTTTTCATTTACGTGACACTATAGGGGACAGGCTACTTGTTCTTTATGCAGCGGC; CGCGAATTCGGCACGAGGATAAACCTTCCCTCCCATGGATAATAGTGCTAATTCCTGAGG; ACCTGAAGGGCCTGCCGCCCCTGGGGGATTAGCCAGAAGCAGGCTTGTTTTCCTGCTCAG; AACAAAGGGAGTTCCCTGAACACATCTTCATTATGATTCACACCAACCTGAAGAAAAAGT; TCAGCTGCTGCGTCCTGGTCTTTCTTCTGTTTGCAGTCATCTGTGTGTGGAAGGAAAAGA; AGAAAGGGAGTTACTATGATTCCTTTAAATTGCAAACCAAGGAATTCCAGGTGTTAAAGA; GTCTGGGGAAATTGGCCATGGGGTCTGATTCCCAGTCTGTATCCTCAAGCAGCACCCAGG; ACCCCCACAGGGGCCGCCAGACCCTCGGCAGTCTCAGAGGCCTAGCCAAGGCCAAACCAG; AGGCCTCCTTCCAGGTGTGGAACAAGGACAGCTCTTCCAAAAACCTTATCCCTAGGCTGC; AAAAGATCTGGAAGAATTACCTAAGCATGAACAAGTACAAAGTGTCCTACAAGGGGCCAG; GACCAGGCATCAAGTTCAGTGCAGAGGCCCTGCGCTGCCACCTCCGGGACCATGTGAATG; TATCCATGGTAGAGGTCACAGATTTTCCCTTCAATACCTCTGCATGGGAGGGTTATCTGC; TCAAGGAGAGCATTAGGACCAAGGCTGGGCCTTGGGGCACGTGTGCTGTTGTGTCGTCAG; CGGGATCTCTTGAAGTCTNCCTACTAGGCAGAGACATCGATGATCTGGACGCAGTCCTGA; GGGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003032 unedited; GTTTACCAGAAATACTATGNACCGCGCCGCTTTCTANNGATCGGTTTTTTTTTTTTTTTT; TTAAAGAACACACACACATTTATTTTATAATTTGACATAATCCTTGGGTAATTATTAGCA; TTTTTCAGAAGTTTCCGAGACTGATATCTCAACTCCAAACCAATAGCAATAGCTTATTTG; ATTGGTGGAAGGATTAGAGAGGGTCCCATGATCCAGATGAATAGATGGCTATCAAAGAAG; ACAGAACCCCATAGACACAGGCAGCATTAAGTCATAATTTTTAAATTAACTGTCACAAAT; ATGAAACATCTCACCAGACCAATGATAACGTCCATATCATAATTTGCATTTTACCAAAGT; GTCCACATAGACAGCACAGAACTAAAAGTTCTGTAGACTTCAGAATCATATTTAAATTAA; TTTGATCCTTTATATTAATAATAATTAAAAACTAGATTCTACCCAGTGTCGTCCCAGTCT; TCACACCAACATGTATGTCCTGCTGTCGGAATGCCACCTCCTCTGCACTCAGTGTCATGA; TTCGAAGATTAAAATCAGTGTGTCAAATCAGAACATAAACCAAGCCAGGAATTCATGGGC; CCAGAGAGGGGTAAGGGGCTTGTCCTATACAGCAGTTAAGCAGCCCACGTGCTCCGCCCA; TTCCCAAGCAGAATCAGATTGGAGCTGCANGCANCGAGGAGGAGGGCGTGTTCTGTGTTG; TCCACTGCTTTGCATGGTTTCTTGGGTGTTCCAAGGAGGACAGTTGTTGTCTCCCATAAG; ACAGTTAATTCCCTGAGGACGGACCTCTTNCAACTAGCCTNCGTCACAGCAGGGTGCATG; CAGNCCGAGTGTGAGCAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_003032
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003032.2, NP_003023.1
• RefSeq Size: 4295 bp
• RefSeq ORF: 1221 bp
• Locus ID: 6480
• UniProt ID: P15907
• Cytogenetics: 3q27.3
• Domains: Glyco_transf_29
• Protein Families: Secreted Protein
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Summary: This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. The promoter and 5' terminal exon sequence is from an endogenous retroviral LTR (PMID: 8724135). Variant 2 is expressed in mature B lymphocytes and variants 1, 2 and 4 encode the same protein (isoform a).