Nudel (NDEL1) (NM_030808) Human Untagged Clone

SKU SC109646

NDEL1 (untagged)-Human nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Nudel
• Synonyms: EOPA; MITAP1; NDE1L1; NDE2; NUDEL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109646 sequence for NM_030808 edited (data generated by NextGen Sequencing); ATGGATGGTGAAGATATACCAGATTTTTCAAGTTTAAAGGAGGAAACTGCTTATTGGAAG; GAACTTTCCTTGAAGTATAAGCAAAGCTTCCAGGAAGCTCGGGATGAGCTAGTTGAATTC; CAGGAAGGAAGCAGAGAATTAGAAGCAGAGTTGGAGGCACAATTAGTACAGGCTGAACAA; AGAAATAGAGACTTGCAGGCTGATAACCAAAGACTGAAATATGAAGTGGAGGCATTAAAG; GAGAAGCTAGAGCATCAATATGCACAGAGCTATAAGCAGGTCTCAGTGTTAGAAGATGAT; TTAAGTCAGACTCGGGCCATTAAGGAGCAGTTGCATAAGTATGTGAGAGAGCTGGAGCAG; GCCAACGACGACCTGGAGCGAGCCAAAAGGGCAACAATAGTTTCACTGGAAGACTTTGAA; CAAAGGCTAAACCAGGCCATTGAACGAAATGCATTTTTAGAAAGTGAACTTGATGAAAAG; GAATCTTTGTTGGTCTCTGTACAGAGGTTAAAGGATGAAGCAAGAGATTTAAGGCAAGAA; CTAGCAGTTCGGGAAAGACAACAGGAAGTAACTAGAAAGTCGGCTCCTAGCTCTCCAACT; CTAGACTGTGAAAAGATGGACTCCGCCGTCCAAGCATCACTTTCTTTGCCAGCTACCCCT; GTTGGCAAAGGAACGGAGAACACTTTTCCTTCACCGAAAGCTATACCAAATGGTTTTGGT; ACCAGTCCACTAACTCCCTCTGCTAGGATATCAGCACTAAACATCGTGGGGGATCTCTTA; CGGAAAGTAGGGGCTTTAGAATCCAAATTAGCAGCTTGCAGGAATTTTGCAAAGGACCAA; GCATCACGAAAATCCTATATTTCAGGGAATGTTAACTGTGGGGTGCTGAATGGCAATGGC; ACAAAGTTCTCTCGATCAGGGCATACATCTTTCTTCGACAAAGGGGCAGTAAACGGCTTT; GACCCCGCTCCTCCTCCTCCTGGTCTGGGCTCCTCGCGTCCATCGTCAGCGCCGGGTATG; CTGCCTCTCAGTGTGTGA; ; Clone variation with respect to NM_030808.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030808 unedited; TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCGGACACCCAGGC; AGTCCCTGACGTGTCGGGGAGGAGCCGGGCGCGGAGGTACGCTGAGTGGAGCTCGGGGCT; GCGTAGGGGAGCTGAGCCGAGCGGCTGGGCGGGCCTGGCCGGGCCAGCGGAGGGGAGACG; TCGGTTGAGCGGCGGCGAACATGCGCTTTTGACACATTGGAGGCTTTCTTGATCATGGAT; GGTGAAGATATACCAGATTTTTCAAGTTTAAAGGAGGAAACTGCTTATTGGAAGGAACTT; TCCTTGAAGTATAAGCAAAGCTTCCAGGAAGCTCGGGATGAGCTAGTTGAATTCCAGGAA; GGAAGCAGAGAATTAGAAGCAGAGTTGGAGGCACAATTAGTACAGGCTGAACAAAGAAAT; AGAGACTTGCAGGCTGATAACCAAAGACTGAAATATGAAGTGGAGGCATTAAAGGAGAAG; CTAGAGCATCAATATGCACAGAGCTATAAGCAGGTCTCAGTGTTAGAAGATGATTTAAGT; CAGACTCGGGCCATTAAGGAGCAGTTGCATAAGTATGTGAGAGAGCTGGAGCAGGCCAAC; GACGACCTGGAGCGAGCCAAAAGGGCAACAATAGTTTCACTGGAAGACTTTGAACAAAGG; CTAAACCAGGCCATTGAACGAAATGCATTTTTAGAAAGTGAACTTGATGAAAAGGAATCT; TTNGTTGGTCTCTGTACAGAGGTTAAAGGATGAAGNCAGAGATTTAGGGCAGAACTAGCA; GTTCGGGAAAAGACACAGGAAGTACTAGAAAGTCGGCTCCTAGCTCTNCACTCTAGACTG; TGAAAGATGGACTNNCGCGTCCAGCATCACTTTCTTTGCAGCTACCCCTGTTGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030808 unedited; CTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTTTTTTCTGTGAAATTTATTGTTTCCAC; ATTAAAAGAATTTTTTTGAGGGAAGTATTTTTTGTAACCACATGATGATCCTATACAGCT; GCCCAGAGGAGGACACCATCAGAGTCATTAACATGCCGTATGCGTTCTGAATAAATAAAA; GCTACAAAGCCCAAGTTATATACAAATATCTCAGGCAATAATGTTTACAAACCTATGTAC; AATAAAACAAATGTAAACAGTAAATGCAGCATGAGAGTAATCAAAGGATGCACAGGGACA; GCCGCCGCTGTTCAGTCCACCCCGGAGCTGGCACCGGCCCACCCTGGAACCAGCCACTCA; CCCTTCCTAACACAACGGGACGGCCCTTGGAAGAGAAAACAAAAACACTCCAAAGATGTC; GTTGAACTGAAGGACGGGAAGGCTGGCGAGAGGCTGCCCGCCTCCCTCACCCCGAGTCTG; AGGCGCACAGAGGAGGAGACAGCACAGAGGCTGGGCCTGTGTGTGCCCCGTCCCAGAGGC; TCCTGCCACGTGGGGCTCAGACGGGCATTGTGCTGCTCTGGGAGGCAAGTCCATGGAATG; GTANCTGCTTAAGTAAATCAGAGGTGCAGAANNATGTCCCTAATCAGTACCTTCCTGACT; CTTGCCAGAACAGGCCAAACTGTGAGGGGTGGNAGTGGGGACACATGATCATGAGCCTGG; AGATGCCTGGGTGGGAGCTCAGGAGGCACACATTGACCTGATTGGGGGGAGACTNCTAGA; GAATTGCAATGTCCTAAACACACTAATCCTATANTACANAAAGTGGGGGCTATGGNGCAN; GTGTGATGATGGGTACTTCTGCATCAGTGACCAGTATACACGTTGTATCACCTGCGACCA; AAAGTGCATCATGGGACCACGGCCTACGGAGAGAGAATNCCGATTGATCGGCAAGTCCGG; GCTGGCAGGNCCATANCGCATGATCANCTGCTTTTCACCGGGGAGAACGAGGCCAGGCTG; CCANACCAGGGGAGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_030808
• Insert Size: 2350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030808.3, NP_110435.1
• RefSeq Size: 2385 bp
• RefSeq ORF: 1038 bp
• Locus ID: 81565
• UniProt ID: Q9GZM8
• Cytogenetics: 17p13.1
• Domains: NUDE_C
• Summary: This gene encodes a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization, cell signaling and neuron migration, outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Mar 2012]; Transcript Variant: This variant (2) lacks an exon in the 3' coding region, compared to variant 1. The encoded isoform (B) is longer and has a distinct C-terminus, compared to isoform A.