SEPTIN4 (NM_004574) Human Untagged Clone

SKU SC109592

41521 (untagged)-Human septin 4 (SEPT4), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SEPTIN4
• Synonyms: ARTS; BRADEION; C17orf47; CE5B3; H5; hCDCREL-2; hucep-7; MART; PNUTL2; SEP4; SEPT4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004574 edited; ATGGACCGTTCACTGGGATGGCAAGGGAATTCTGTCCCTGAGGACAGGACTGAAGCTGGG; ATCAAGCGTTTCCTGGAGGACACCACGGATGATGGAGAACTGAGCAAGTTCGTGAAGGAT; TTCTCAGGAAATGCGAGCTGCCACCCACCAGAGGCTAAGACCTGGGCATCCAGGCCCCAA; GTCCCGGAGCCAAGGCCCCAGGCCCCGGACCTCTATGATGATGACCTGGAGTTCAGACCC; CCCTCGCGGCCCCAGTCCTCTGACAACCAGCAGTACTTCTGTGCCCCAGCCCCTCTCAGC; CCATCTGCCAGGCCCCGCAGCCCATGGGGCAAGCTTGATCCCTATGATTCCTCTGAGGAT; GACAAGGAGTATGTGGGCTTTGCAACCCTCCCCAACCAAGTCCACCGAAAGTCCGTGAAG; AAAGGCTTTGACTTTACCCTCATGGTGGCAGGAGAGTCTGGCCTGGGCAAATCCACACTT; GTCAATAGCCTCTTCCTCACTGATCTGTACCGGGACCGGAAACTTCTTGGTGCTGAAGAG; AGGATCATGCAAACTGTGGAGATCACTAAGCATGCAGTGGACATAGAAGAGAAGGGTGTG; AGGCTGCGGCTCACCATTGTGGACACACCAGGTTTTGGGGATGCAGTCAACAACACAGAG; TGCTGGAAGCCTGTGGCAGAATACATTGATCAGCAGTTTGAGCAGTATTTCCGAGACGAG; AGTGGCCTGAACCGAAAGAACATCCAAGACAACAGGGTGCACTGCTGCCTGTACTTCATC; TCACCCTTCGGCCATGGGCTCCGGCCATTGGATGTTGAATTCATGAAGGCCCTGCATCAG; CGGGTCAACATCGTGCCTATCCTGGCTAAGGCAGACACACTGACACCTCCCGAAGTGGAC; CACAAGAAACGCAAAATCCGGGAGGAGATTGAGCATTTTGGAATCAAGATCTATCAATTC; CCAGACTGTGACTCTGATGAGGATGAGGACTTCAAATTGCAGGACCAAGCCCTAAAGGAA; AGCATCCCATTTGCAGTAATTGGCAGCAACACTGTAGTAGAGGCCAGAGGGCGGCGAGTT; CGGGGTCGACTCTACCCCTGGGGCATCGTGGAAGTGGAAAACCCAGGGCACTGCGACTTT; GTGAAGCTGAGGACAATGCTGGTACGTACCCACATGCAGGACCTGAAGGATGTGACGCGG; GAGACACATTATGAGAACTACCGGGCACAGTGCATCCAGAGCATGACCCGCCTGGTGGTG; AAGGAACGGAATCGCAACAAACTGACTCGGGAAAGTGGTACCGACTTCCCCATCCCTGCT; GTCCCACCAGGGACAGATCCAGAAACTGAGAAGCTTATCCGAGAGAAAGATGAGGAGCTG; CGGCGGATGCAGGAGATGCTACACAAAATACAAAAACAGATGAAGGAGAACTATTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004574 unedited; GGTTCANCAATATGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGCTGT; TTCTGTGTGTGAGGAACTTTGCCTGGGAGATAAAATTAGACCTAGAGCTTTCTGACAGGG; AGTCTGAAGCGTGGGACATGGACCGTTCACTGGGATGGCAAGGGAATTCTGTCCCTGAGG; ACAGGACTGAAGCTGGGATCAAGCGTTTCCTGGAGGACACCACGGATGATGGAGAACTGA; GCAAGTTCGTGAAGGATTTCTCAGGAAATGCGAGCTGCCACCCACCAGAGGCTAAGACCT; GGGCATCCAGGCCCCAAGTCCCGGAGCCAAGGCCCCAGGCCCCGGACCTCTATGATGATG; ACCTGGAGTTCAGACCCCCCTCGCGGCCCCAGTCCTCTGACAACCAGCAGTACTTCTGTG; CCCCAGCCCCTCTCAGCCCATCTGCCAGGCCCCGCAGCCCATGGGGCAAGCTTGATCCCT; ATGATTCCTCTGAGGATGACAAGGAGTATGTGGGCTTTGCAACCCTCCCCAACCAAGTCC; ACCGAAAGTCCGTGAAGAAAGGCTTTGACTTTACCCTCATGGTGGCAGGAGAGTCTGGCC; TGGGCAAATCCACACTTGTCAATAGCCTCTTCCTCACTGATCTGTACCGGGACCGGAACT; TCCTTGGTGCTGAAGAGAGGATCATGCAAACTGTGGAGATCACTAAGCATGCAGTGGACA; TANAAAAGAAGGGTGTGAGGCTGCGGCTCACCATTGTTNACACACCAGTTTTTTGGGATG; CAGTCAACACCACAGAGTGCTGGGAACCTGTGGCAGATACATTGATCAGCAGNTTGAGCA; GTATTTTCGAACCAAAGTGGCCTTGACCGAAGAACATTCAAGACAACAGGTGCACTGCTG; GCTGTACTCATTTCACCTTTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004574 unedited; CTTTGGACCGCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTACAAATTTAT; TAAACTTTATTTCCTCTGAGTCTCTGGGCAGTCAGCAGGGATGTAAGGCGAAGTGGCAGT; AGCTGAAGGGGCCTGAGCAGAGCTGGTGCTGGGAGGGGCCGGCATGGACAGGAAGAAGAG; GAGGAGATTTAAATATCCAGGGCTGAAAGCCAGTTAATAGTTCTCCTTCATCTGTTTTTG; TATTTTGTGTAGCATCTCCTGCATCCGCCGCAGCTCCTCATCTTTCTCTCGGATAAGCTT; CTCAGTTTCTGGATCTGTCCCTGGTGGGACAGCAGGGATGGGGAAGTCGGTACCACTTTC; CCGAGTCAGTTTGTTGCGATTCCGTTCCTTCACCACCAGGCGGGTCATGCTCTGGATGCA; CTGTGCCCGGTAGTTCTCATAATGTGTCTCCCGCGTCACATCCTTCAGGTCCTGCATGTG; GGTACGTACCAGCATTGTCCTCAGCTTCACAAAGTCGCAGTGCCCTGGGTTTTCCACTTC; CACGATGCCCCAGGGGTAGAGTCGACCCCGAACTCGCCGCCCTCTGGCCTCTACTACAGT; GTTGCTGCCAATTACTGCAAATGGGATGCTTTCCTTTNAGGCTTGGTCCTGCAATTTGAA; GTCCTCATCCTCATCAGAGTCACAGTCTGGGAATTGATAGATCTTGATTCCAAATGCTCA; ATCTCCTCCCGGATTTTGCGTTTCTTGTGGTCCACTTCGGGAGGTGTCAGTGTGTCTGCC; TTAACCAGATAGCACGATGTTGACCCGCTGATGCAGGNCCTTCATGAATTCACATTCCAT; GGCCGNAGCCATGGCCGANGGTTGANATGAANTCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004574
• Insert Size: 1820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004574.2, NP_004565.1
• RefSeq Size: 1767 bp
• RefSeq ORF: 1437 bp
• Locus ID: 5414
• UniProt ID: O43236
• Cytogenetics: 17q22
• Domains: GTP_CDC
• Summary: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. [provided by RefSeq, Nov 2010]; Transcript Variant: This variant (1) differs in the 5' UTR and coding region and initiates translation at an alternate start codon, compared to variant 7. It encodes isoform 1 (also known as PNUTL2a, H5, SEPT4_i1, bradeion beta, hcdcrel2a) which contains a distinct N-terminus and is shorter, compared to isoform 5.