TDRD3 (NM_030794) Human Untagged Clone

SKU SC109535

TDRD3 (untagged)-Human tudor domain containing 3 (TDRD3), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: TDRD3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_030794, the custom clone sequence may differ by one or more nucleotides; ; ; ATGCTGCGATTACAGATGACTGATGGTCATATAAGTTGCACAGCAGTAGAATTTAGTTATATGTCAAAAA; TAAGCCTGAACACACCACCTGGAACTAAAGTTAAGCTCTCAGGCATTGTTGACATAAAAAATGGATTCCT; GCTCTTGAATGACTCTAACACCACAGTTCTTGGTGGTGAAGTGGAACACCTTATTGAGAAATGGGAGTTA; CAGAGAAGCTTATCAAAACACAATAGAAGCAATATTGGAACTGAAGGTGGACCACCGCCTTTTGTGCCTT; TTGGACAGAAGTGTGTATCTCATGTCCAAGTGGATAGCAGAGAACTTGATCGAAGAAAAACATTGCAAGT; TACAATGCCTGTCAAACCTACAAATGATAATGATGAATTTGAAAAGCAAAGGACGGCTGCTATTGCTGAA; GTTGCAAAGAGCAAGGAAACCAAGACATTTGGAGGAGGTGGTGGTGGTGCTAGAAGTAATCTCAATATGA; ATGCTGCTGGTAACCGAAATAGGGAAGTTTTACAGAAAGAAAAGTCAACCAAATCAGAGGGAAAACATGA; AGGTGTCTATAGAGAACTGGTTGATGAGAAAGCTCTGAAGCACATAACGGAAATGGGCTTCAGTAAGGAA; GCATCGAGGCAAGCTCTTATGGATAATGGCAACAACTTAGAAGCAGCACTGAACGTACTTCTTACAAGCA; ATAAACAGAAACCTGTTATGGGTCCTCCTCTGAGAGGTAGAGGAAAAGGCAGGGGGCGAATAAGATCTGA; AGATGAAGAGGACCTGGGAAATGCAAGGCCATCAGCACCAAGCACATTATTTGATTTCTTGGAATCTAAA; ATGGGAACTTTGAATGTGGAAGAACCTAAATCACAGCCACAGCAGCTTCATCAGGGACAATACAGATCAT; CAAATACTGAGCAAAATGGAGTAAAAGATAATAATCATCTGAGACATCCTCCTCGAAATGATACCAGGCA; GCCAAGAAATGAAAAACCGCCTCGTTTTCAAAGAGACTCCCAAAATTCAAAGTCAGTTTTAGAAGGCAGT; GGATTACCTAGAAATAGAGGTTCTGAAAGACCAAGTACTTCTTCAGTATCTGAAGTATGGGCTGAAGACA; GAATCAAATGTGATAGACCGTATTCTAGATATGACAGAACTAAAGATACTTCATATCCTTTAGGTTCTCA; GCATAGTGATGGTGCTTTTAAAAAAAGAGATAACTCTATGCAAAGCAGATCAGGAAAAGGTCCCTCCTTT; GCAGAGGCAAAAGAAAATCCACTTCCTCAAGGATCTGTAGATTATAATAATCAAAAACGTGGAAAAAGAG; AAAGCCAAACATCTATTCCTGATTATTTTTATGACAGGAAATCACAAACAATAAATAATGAAGCTTTCAG; TGGTATAAAAATTGAAAAACATTTTAATGTAAATACTGATTATCAGAATCCAGTTCGAAGTAATAGTTTC; ATTGGTGTTCCAAATGGAGAAGTAGAAATGCCACTGAAAGGAAGACGAATAGGACCTATTAAGCCAGCAG; GACCTGTCACAGCTGTACCCTGTGATGATAAAATATTTTACAATAGTGGGCCCAAACGAAGATCTGGGCC; AATTAAGCCAGAAAAAATACTAGAATCATCTATTCCTATGGAGTATGCAAAAATGTGGAAACCTGGAGAT; GAATGTTTTGCACTTTATTGGGAAGACAACAAGTTTTACCGGGCAGAAGTTGAAGCCCTCCATTCTTCGG; GTATGACAGCAGTTGTTAAATTCATTGACTACGGAAACTATGAAGAGGTGCTACTGAGCAATATCAAGCC; CATTCAAACAGAGGCATGGGAGGAAGAAGGCACCTACGATCAAACTCTGGAGTTCCGTAGGGGAGGTGAT; GGCCAGCCAAGACGATCCACTCGGCCAACCCAACAGTTTTACCAACCACCCCGGGCTCGGAACTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030794 unedited; NGTAACGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACNGGGGGG; GGGTCTCAAGTAGGAGGCCTCCCCATCACCCCCACCCCAGCCCCCCACCACCCCCGGCCT; AAGCAGCTACCATGGCCCAGGTGGCCGGCGCGGCGTTGTCCCAGGCGGGTTGGTATCTTT; CAGATGAAGGCATTGAAGCTTGCACAAGCTCTCCAGACAAAGTCAATGTAAATGACATCA; TCCTGATTGCTCTCAATACAGATCTGAGAACAATTGGCAAGAAATTCCTCCCCAGTGACA; TCAATAGTGGAAAGGTAGAAAAGCTCGAAGGTCCATGTGTTTTGCAAATTCAAAAAATTC; GCAATGTTGCTGCACCAAAGGATAATGAAGAATCTCAGGCTGCACCAAGGATGCTGCGAT; TACAGATGACTGATGGTCATATAAGTTGCACAGCAGTAGAATTTAGTTATATGTCAAAAA; TAAGCCTGAACACACCACCTGGAACTAAAGTTAAGCTCTCAGGCATTGTTGACATAAAAA; ATGGATTCCTGCTCTTGAATGACTCTAACACCACAGTTCTTGGTGGTGAAGTGGAACACC; TTATTGAGAAATGGGAGTTACAGAGAAGCTTATCAAAACACAATAGAAGCAATATTGGAA; CTGAAGGTGGACCACCGCCTTTTGTGCCTTTTGGACAGAAGTGTGTATCTCATGTCCAAG; TGGATAGCAGAGAACTTGATCGAAGAAAAACATTGCAAGTTACAATGCCTGTCAAACCTA; CAAATGATAATGATGAATTTGAAAAGCANAGGACGGCTGCTATTTGCTGAATTTGCAAGA; GCAAGGAAACCAAGACATTTGGAGGAGGGGTTGGTGGGTTGCTAGAAATAATC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030794 unedited; ACATGGTTTTCTTGACGGCAGNAANAAAATTCTTCCTTTCACTTGTAAAAGTATTTGCTT; AATTTAACTCAGTATTTTTTTCCATTCTTTGTGTGAGTTGACTGTTTTTTCCACCCTAAA; ATCTGTTTTTTCTTTCTTCAAATAATAATATCCACCACAGCTACTTATTCTGAAGAGAAA; GTGGAAGGTCTGTCAACAGGTTTCCACCAGGGTGTTTCAGTCACTGGCTCGTTTCTTCAC; AAAGAGTCTACTTTTCCTATTAGTTCCGAGCCCGGGGTGGTTGGTAAAACTGTTGGGTTG; GCCGAGTGGATCGTCTTGGCTGGCCATCACCTCCCCTACGGAACTCCAGAGTTTGATCGT; AGGTGCCTTCTTCCTCCCATGCCTCTGTTTGAATGGGCTTGATATTGCTCAGTAGCACCT; CTTCATAGTTTCCGTAGTCAATGAATTTAACAACTGCTGTCATACCCGAAGAATGGATGG; CTTCAACTTCTGCCCGGTAAAACTTGTTGTCTTCCCAATAAAGTGCAAAACATTCATCTC; CAGGTTTCCACATTTTTGCATACTCCATAGGAATAGATGATTCTAGTATTTTTTCTGGCT; TAATTGGCCCAGATCTTCGTTGGGCGCACTATTGTAAATATTTTATCATCACAGGGTACA; GCTGTGACAGGTCCTGCTGGCTTATAGGTCCATCCGTCCTCCTTTCAAGGCTTTTCTACT; TCTCCTTTTGGACACCATTGAAACATTACTTCCAACTGGATTCTGTAATCCATATTACCT; TAAAAAGGGTCCCAATTTTATACCACGGAAAGCTTCATTATTATTGCTTGTGATTTCCCG; CCTAAAATCATCAGAATAGAGTTTGGCTTTTTCTTTTCACATTGTTGATTTTCTAATCCA; CAAATCCCGGAGGAATAGATTTCCCTTGGCCCCTGCAAGGAAGGCCCCTCCCTCACCCCC; CCG;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_030794
• Insert Size: 2950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030794.1, NP_110421.1
• RefSeq Size: 2501 bp
• RefSeq ORF: 1956 bp
• Locus ID: 81550
• UniProt ID: Q9H7E2
• Cytogenetics: 13q21.2
• Domains: TUDOR, UBA
• Protein Families: Druggable Genome
• Summary: Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) contains a distinct 5' UTR and uses a different splice site in its 5' UTR, compared to variant 1. These differences cause translation initiation at a downstream start codon. The encoded protein (isoform 2) is shorter than isoform 1.