MMP19 (NM_002429) Human Untagged Clone

SKU SC109442

MMP19 (untagged)-Human matrix metallopeptidase 19 (MMP19), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: MMP19
• Synonyms: CODA; MMP18; RASI-1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002429 edited; GCTGGAGAGTAAAAAGAACTGGGGGTAAGAGCCCCTCTGCCTAGCACTGCTCCCCCAAGG; CTCCCAGAAATCTCAGGTCAGAGGCACGGACAGCCTCTGGAGCTCTCGTCTGGTGGGACC; ATGAACTGCCAGCAGCTGTGGCTGGGCTTCCTACTCCCCATGACAGTCTCAGGCCGGGTC; CTGGGGCTTGCAGAGGTGGCGCCCGTGGACTACCTGTCACAATATGGGTACCTACAGAAG; CCTCTAGAAGGATCTAATAACTTCAAGCCAGAAGATATCACCGAGGCTCTGAGAGCTTTT; CAGGAAGCATCTGAACTTCCAGTCTCAGGTCAGCTGGATGATGCCACAAGGGCCCGCATG; AGGCAGCCTCGTTGTGGCCTAGAGGATCCCTTCAACCAGAAGACCCTTAAATACCTGTTG; CTGGCCCGGGCAGCCCTGCGTCAAGCCTTCCAGGACTGGAGCAATGTGGCTCCCTTGACC; TTCCAAGAGGTGCAGGCTGGTGCGGCTGACATCCGCCTCTCCTTCCATGGCCGCCAAAGC; TCGTACTGTTCCAATACTTTTGATGGGCCTGGGAGAGTCCTGGCCCATGCCGACATCCCA; GAGCTGGGCAGTGTGCACTTCGACGAAGACGAGTTCTGGACTGAGGGGACCTACCGTGGG; GTGAACCTGCGCATCATTGCAGCCCATGAAGTGGGCCATGCTCTGGGGCTTGGGCACTCC; CGATATTCCCAGGCCCTCATGGCCCCAGTCTACGAGGGCTACCGGCCCCACTTTAAGCTG; CACCCAGATGATGTGGCAGGGATCCAGGCTCTCTATGGCAAGAAGAGTCCAGTGATAAGG; GATGAGGAAGAAGAAGAGACAGAGCTGCCCACTGTGCCCCCAGTGCCCACAGAACCCAGT; CCCATGCCAGACCCTTGCAGTAGTGAACTGGATGCCATGATGCTGGGGCCCCGTGGGAAG; ACCTATGCTTTCAAGGGGGACTATGTGTGGACTGTATCAGATTCAGGACCGGGCCCCTTG; TTCCGAGTGTCTGCCCTTTGGGAGGGGCTCCCCGGAAACCTGGATGCTGCTGTCTACTCG; CCTCGAACACAATGGATTCACTTCTTTAAGGGAGACAAGGTGTGGCGCTACATTAATTTC; AAGATGTCTCCTGGCTTCCCCAAGAAGCTGAATAGGGTAGAACCTAACCTGGATGCAGCT; CTCTATTGGCCTCTCAACCAAAAGGTGTTCCTCTTTAAGGGCTCCGGGTACTGGCAGTGG; GACGAGCTAGCCCGAACTGACTTCAGCAGCTACCCCAAACCAATCAAGGGTTTGTTTACG; GGAGTGCCAAACCAGCCCTCGGCTGCTATGAGTTGGCAAGATGGCCGAGTCTACTTCTTC; AAGGGCAAAGTCTACTGGCGCCTCAACCAGCAGCTTCGAGTAGAGAAAGGCTATCCCAGA; AATATTTCCCACAACTGGATGCACTGTCGTCCCCGGACTATAGACACTACCCCATCAGGT; GGGAATACCACTCCCTCAGGTACGGGCATAACCTTGGATACCACTCTCTCAGCCACAGAA; ACCACGTTTGAATACTGACTGCTCACCCACAGACACAATCTTGGACATTAACCCCTGAGG; CTCCACCACCCACCCTTTCATTTCCCCCCCAGAAGCCTAAGGCCTAATAGCTGAATGAAA; TACCTGTCTGCTCAGTAGAACCTTGCAGGTGCTGTAGCAGGCGCAAGACCGTAGATCTCA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002429 unedited; GTCATTCCCCCGCCCCTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA; AGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCG; CGAATTCGGCACGAGAGAGGCTGGAGAGTAAAAAGAACTGGGGGTAAGAGCCCCTCTGCC; TAGCACTGCTCCCCCAAGGCTCCCAGAAATCTCAGGTCAGAGGCACGGACAGCCTCTGGA; GCTCTCGTCTGGTGGGACCATGAACTGCCAGCAGCTGTGGCTGGGCTTCCTACTCCCCAT; GACAGTCTCAGGCCGGGTCCTGGGGCTTGCAGAGGTGGCGCCCGTGGACTACCTGTCACA; ATATGGGTACCTACAGAAGCCTCTAGAAGGATCTAATAACTTCAAGCCAGAAGATATCAC; CGAGGCTCTGAGAGCTTTTCAGGAAGCATCTGAACTTCCAGTCTCAGGTCAGCTGGATGA; TGCCACAAGGGCCCGCATGAGGCAGCCTCGTTGTGGCCTAGAGGATCCCTTCAACCAGAA; GACCCTTAAATACCTGTTGCTGGCCCGGGCAGCCCTGCGTCAAGCCTTCCAGGACTGGAG; CAATGTGGCTCCCTTGACCTTCCAAGAGGTGCAGGCTGGTGCGGCTGACATCCGCCTCTC; CTTCCATGGCCGCCAAAGCTCGTACTGTTCCAATACTTTTGATGGGCCTGGGAGAGTCCT; GGCCCATGCCGACATCCCAGAGCTGGGCAGTGTGCACTTCGACGAAGACGAGTTCTGGAC; TGANGGGACCTACCGTNGGGTGAACCTGCGCATCATTGCAGCCCATGAAGTGNGCCATGC; TCTGGGGCTTGNGCACTCCCGATATTCCCAGGCCCTCATGGCCCCAGTCTACGAGGGCTA; CCGGCCCCNACTTTAGCTGCACCCAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002429 unedited; NNNCGGNTTAACTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTTT; TTTTTTTTATGAACATGGGAGTTAGCTTTTATTAAACACAAATGTACCAGACACTGTGCC; AGGCACTTCACATACAGTATTTCATTTAGTGCAACAATCCTGCAGTACTGGTCTTAACCT; AATGTTGCAAATGGGGAATCTGAGATTCAACAAGGTTAATTAGCTTGCCCGTAATCATAA; GCACATAAATGTGATTCTCAAGGATTCCAAGGCCCTTGCTCAGTTTACTGGCCATGCTGT; TTTCTTGCATTTTATGTAGGAAGAACAGGACCCAGGCATCTCCCTCCACCATTGACCTCC; AGAGAAGAGATGACACAGTTGGAAGGGCTGTCTAAGACAGACAGGAAATGGAGTTGGGGG; CCAAATCTAAGTTAGGGGATCTGAGTTAGGGGAGCACTTCTCAGGAGTGAAAATGCACAG; GAAAGTGGTGGCTGGAGTTGGAAGTGTTAGAGGCCTGAGATCTACGGTCTTGCGCCTGCT; ACAGCACCTGCAAGGTTCTACTGAGCAGACAGGTATTTCATTCAGCTATTAGGCCTTAAG; CTTCTGGGGGGGAAATGAAAGGGGGGTGGGGGGACCTCAAGGGTTAATGTCCCAGAATGT; GTCTGGTGGTGAGCACTCCATATTTCAACGTGGTTTCTGTGGCTGAGAAAGTGGATCCCA; GGTTTTTCCCCTTCCCTGAGGGAGTGGTTTCCCACCTGAGGGGTTAATGGCCTTTGTCCG; GGGACCAACCGGGCTTCCCTTTTTGGGAAAAAATTCTGGGGAAAGGCCTTTCTTTTTTCA; AAACTCCTGGGTTGAGGCCCCCATTTGACTTTTGCCCTTGAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002429
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002429.3, NP_002420.1
• RefSeq Size: 3260 bp
• RefSeq ORF: 1527 bp
• Locus ID: 4327
• UniProt ID: Q99542
• Cytogenetics: 12q13.2
• Domains: hemopexin, Peptidase_M10
• Protein Families: Protease, Secreted Protein
• Summary: This gene encodes a member of a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded protein is secreted as an inactive proprotein, which is activated upon cleavage by extracellular proteases. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Jan 2013]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1, also known as rasi-1).