DNA Ligase III (LIG3) (NM_013975) Human Untagged Clone

CAT#: SC109386

LIG3 (untagged)-Human ligase III, DNA, ATP-dependent (LIG3), nuclear gene encoding mitochondrial protein, transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNA Ligase III
• Synonyms: LIG2; LIG3alpha
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_013975 edited
  ATTTAAAGAGACAGGCGCTCCAACCGTCGTGGGCTGCCCGCGGCCTGTAATGAGCAAGTT
  CCGAGGCCTACGGTGAGCGCCGGAGCCGGAGAGGCAGCTATATGTCTTTGGCTTTCAAGA
  TCTTCTTTCCACAAACCCTCCGTGCACTCAGCCGAAAAGAACTGTGCCTATTCCGAAAAC
  ATCACTGGCGTGATGTAAGACAATTCAGCCAGTGGTCAGAAACAGATCTGCTTCATGGAC
  ATCCCCTCTTCCTGAGAAGAAAGCCTGTTCTATCATTCCAGGGAAGCCATCTAAGATCAC
  GTGCCACCTACCTTGTTTTCTTGCCAGGGTTGCATGTGGGACTCTGCAGTGGCCCCTGTG
  AGATGGCTGAGCAACGGTTCTGTGTGGACTATGCCAAGCGTGGCACAGCTGGCTGCAAAA
  AATGCAAGGAAAAGATTGTGAAGGGCGTATGCCGAATTGGCAAAGTGGTGCCCAATCCCT
  TCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCACATTAAATGCATGTTTGAGAAAC
  TAGAGCGGGCCCGGGCCACCACAAAAAAAATCGAGGACCTCACAGAGCTGGAAGGCTGGG
  AAGAGCTGGAAGATAATGAGAAGGAACAGATAACCCAGCACATTGCAGATCTGTCTTCTA
  AGGCAGCAGGTACACCAAAGAAGAAAGCTGTTGTCCAGGCTAAGTTGACAACCACTGGCC
  AGGTGACTTCTCCAGTGAAAGGCGCCTCATTTGTCACCAGTACCAATCCCCGGAAATTTT
  CTGGCTTTTCAGCCAAGCCCAACAACTCTGGGGAAGCCCCCTCGAGCCCCACCCCTAAGA
  GAAGTCTGTCTTCAAGCAAATGTGACCCCAGGCATAAGGACTGTCTGCTACGG:GAGTTT
  CGAAAGTTATGCGCCATGGTGGCCGATAATCCTAGCTACAACACGAAGACCCAGATCATC
  CAGGACTTCCTTCGGAAAGGCTCAGCAGGAGATGGTTTCCACGGTGATGTGTACCTAACA
  GTGAAGCTGCTGCTGCCAGGAGTCATTAAGACTGTTTACAACTTGAACGATAAGCAGATT
  GTGAAGCTTTTCAGTCGCATTTTTAACTGCAACCCAGATGATATGGCACGGGACCTAGAG
  CAGGGTGACGTGTCAGAGACAATCAGAGTCTTCTTTGAGCAGAGCAAGTCTTTCCCCCCA
  GCTGCCAAGAGCCTCCTTACCATCCAGGAAGTGGATGAGTTCCTTCTGCGGCTGTCCAAG
  CTCACCAAGGAGGATGAGCAGCAACAGGCCCTACAGGACATTGCCTCCAGGTGTACAGCC
  AATGACCTTAAATGCATCATCAGGTTGATCAAACATGATCTGAAGATGAACTCAGGTGCA
  AAACATGTGTTAGACGCCCTTGACCCCAATGCCTATGAAGCCTTCAAAGCCTCGCGCAAC
  CTGCAGGATGTGGTGGAGCGGGTCCTTCACAACGCGCAGGAGGTGGAGAAGGAGCCGGGC
  CAGAGACGAGCTCTGAGCGTCCAGGCCTCGCTGATGACACCTGTGCAGCCCATGTTGGCG
  GAGGCCTGCAAGTCCGTTGAGTATGCAATGAAGAAATGTCCCAATGGCATGTTCTCTGAG
  ATCAAGTACGATGGAGAGCGAGTCCAGGTGCATAAGAATGGAGACCACTTCAGCTACTTC
  AGCCGCAGTCTCAAGCCCGTCCTTCCTCACAAGGTGGCCCACTTTAAGGACTACATTCCC
  CAGGCTTTTCCTGGGGGCCACAGCATGATCTTGGATTCTGAAGTGCTTCTGATTGACAAC
  AAGACAGGCAAACCACTGCCCTTTGGGACTCTGGGAGTACACAAGAAAGCAGCCTTCCAG
  GATGCTAATGTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTTGATG
  GACAGACCTCTGTGTGAGCGGCGGAAGTTTCTTCATGACAACATGGTTGAAATTCCAAAC
  CGGATCATGTTCTCAGAAATGAAGCGAGTCACAAAAGCTTTGGACTTGGCTGACATGATA
  ACCCGGGTGATCCAGGAGGGATTGGAGGGGCTGGTGCTGAAGGATGTGAAGGGTACATAT
  GAGCCTGGGAAGCGGCACTGGCTGAAAGTGAAGAAAGACTATTTGAACGAGGGGGCCATG
  GCCGACACAGCTGACCTGGTGGTCCTTGGAGCCTTCTATGGGCAAGGGAGCAAAGGCGGC
  ATGATGTCAATCTTCCTCATGGGCTGCTACGACCCTGGCAGCCAGAAGTGGTGCACAGTC
  ACCAAGTGTGCAGGAGGCCATGATGATGCCACGCTTGCCCGCCTGCAGAATGAACTAGAC
  ATGGTGAAGATCAGCAAGGACCCCAGCAAAATACCCAGCTGGTTGAAGGTCAACAAGATC
  TACTATCCTGACTTCATCGTCCCAGACCCAAAGAAAGCTGCCGTGTGGGAGATCACAGGG
  GCTGAATTCTCCAAATCGGAGGCTCATACAGCTGACGGGATCTCCATCCGATTCCCTCGC
  TGCACCCGAATCCGAGATGATAAGGACTGGAAATCTGCCACTAACCTTCCCCAACTCAAG
  GAACTGTACCAGTTGTCCAAGGAGAAGGCAGACTTCACTGTAGTGGCTGGAGATGAGGGG
  AGCTCCACTACAGGGGGTAGCAGTGAAGAGAATAAGGGTCCCTCAGGGTCTGCTGTGTCC
  CGCAAGGCCCCCAGCAAGCCCTCAGCCAGTACCAAGAAAGCAGAAGGGAAGCTGAGTAAC
  TCCAACAGCAAAGATGGCAACATGCAGACTGCAAAGCCTTCCGCTATGAAGGTGGGGGAG
  AAGCTGGCCACAAAGTCTTCTCCAGTGAAAGTAGGGGAGAAGCGGAAAGCTGCTGATGAG
  ACGCTGTGCCAAACAAAGGTATTGCTGGACATCTTCACTGGGGTGCGGCTTTACTTGCCA
  CCCTCCACACCAGACTTCAGCCGTCTCAGACGCTACTTTGTGGCATTCGACGGGGACCTG
  GTACAGGAATTTGATATGACTTCAGCCACGCACGTGCTGGGTAGCAGGGACAAGAACCCT
  GCGGCCCAGCAGGTCTCCCCAGAGTGGATTTGGGCATGTATCCGGAAACGGAGACTGGTA
  GCTCCCTGCTAGGTTTGCTGTCTTCCCTCTCCCTCAGGCCATACTCTCCTTTACCATACT
  ACTGGACTGGACTCAGGCTGGAGGCAGATAGACACAGTATAGGGGGAATGGGCTTGCTTC
  TCCCAAACCCACCAGTTCTCCACTGTCTCTTCTGGACCAGGAATTAGTTGCTGTGGGTGC
  CACAGCTGAAGTCAGTTTGTCTTGCTGGTTTAAATAGATCTTTCAGAGCTGGGTGCTGGG
  TTTGCCATCTTTTTGTTTTCTTTGAAAAGCAGCTTAGTTACCCTTTTTATAAATAAAATA
  TCTTGCAGTAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013975 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATTTAAAGAGACGGCGCTCCA
  ACCGTCGTGGGCTGCCCGCGGCCTGTAATGAGCAAGTTCCGAGGCCTACGGTGAGCGCCG
  GAGCCGGAGAGGCAGCTATATGTCTTTGGCTTTCAAGATCTTCTTTCCACAAACCCTCCG
  TGCACTCAGCCGAAAAGAACTGTGCCTATTCCGAAAACATCACTGGCGTGATGTAAGACA
  ATTCAGCCAGTGGTCAGAAACAGATCTGCTTCATGGACATCCCCTCTTCCTGAGAAGAAA
  GCCTGTTCTATCATTCCAGGGAAGCCATCTAAGATCACGTGCCACCTACCTTGTTTTCTT
  GCCAGGGTTGCATGTGGGACTCTGCAGTGGCCCCTGTGAGATGGCTGAGCAACGGTTCTG
  TGTGGACTATGCCAAGCGTGGCACAGCTGGCTGCAAAAAATGCAAGGAAAAGATTGTGAA
  GGGCGTATGCCGAATTGGCAAAGTGGTGCCCAATCCCTTCTCAGAGTCTGGGGGTGATAT
  GAAAGAGTGGTACCACATTAAATGCATGTTTGAGAAACTAGAGCGGGCCCGGGCCACCAC
  AAAAAATATCGAGGACCTCACAGAGCTGGAAGGCTNGGAAGAGCTGGAAAGATATGAGAA
  GGAACAGANTACCCAGCACATTGCAGATCTGTCTTCTAGGCAGCAGGTACACCAAAGAAG
  AAAGCTGGTGTCCAGNCTAAAAGTGACACCACTGGNCCAGTGACTTCTCCAGTGAAAGGC
  GCCTCATTTGTCACCAGTACCAATCCCCGGATATTNTCTGGNCTTTTCAGCCCAGCCCCA
  TCACTCTGGGGGAATGCCCNCTCGAGCCCACCCCTAAGAAGAAGTCTGTCTTCAGCAAAT
  GTGACCCCAGCATAAGGACTGCTGCTCACGNAGTTTTCCAAAGTCTGCCCCATGGNGGNC
  GATATCCTATCTCACACGAGAACCAGATCTCCAGACTTCTTCGAAGCTANAAGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013975 unedited
  GACCGCGGGCCGCATTTTAGNATCGAGTTTTTTTTTTTTTTTTTTACTGCAAGATATTTT
  ATTTATAAAAAGGGTAACTAAGCTGCTTTTCAAAGAAAACAAAAAGATGGCAAACCCAGC
  ACCCAGCTCTGAAAGATCTATTTAAACCAGCAAGACAAACTGACTTCAGCTGTGGCACCC
  ACAGCAACTAATTCCTGGTCCAGAAGAGACAGTGGAGAACTGGTGGGTTTGGGAGAAGCA
  AGCCCATTCCCCCTATACTGTGTCTATCTGCCTCCAGCCTGAGTCCAGTCCAGTAGTATG
  GTAAAGGAGAGTATGGCCTGAGGGAGAGGGAAGACAGCAAACCTAGCAGGGAGCTACCAG
  TCTCCGTTTCCGGATACATGCCCAAATCCACTCTGGGGAGACCTGCTGGGCCGCAGGGCT
  CTTGCCCCTGCTACCCAGCACGTGCGTGGCTGAAGTCATATCAAATCCCTGTACCACGCC
  CCCGTCGAATGCCACAAAGTATCGCCCGACACGGCTGAAGTCTGGTGTGGAGGGTGGTAA
  GTAAAGCCGCACCCCAGTGAAGATGTCCAGCAATACCTTTGTTTGGCACAGCGTCTCATC
  ATCAGCTTCTCCGCTTCTCCCCTACTTTCACTGCAGAAGACTTTGTGGCCAGCTTTTCCC
  CCACCTTCATAGCCGAAGGCTTTGCAGCCTGCATGCCGCCATCCCTCGCTGTTGGAGTTA
  CCCAACTTCCCCTTTCCTTTCCTTGTCACTGGCTGCGGGCCTGCTTGGGGGCCCCGCCGG
  ACACAGCACACCCCTGAGGACCCTNACTTTTCTCCACTGGCTACCCCCTGGAGCGCAGCT
  CCCCTTAATCTCCGCCCCTAACGTGAAGTCCGCCTTCATCCTTGGACAACCGGACCACGC
  TTCCTCGATCTCGGGCACAGTTACCGTTATACATTCCATCCTTCACATCTCGGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013975
• Insert Size: 3510 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013975.2, NP_039269.2
• RefSeq Size: 3701 bp
• RefSeq ORF: 3030 bp
• Locus ID: 3980
• UniProt ID: P49916
• Cytogenetics: 17q12
• Domains: DNA_ligase, BRCT, zf-PARP
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha).