RNF170 (NM_030954) Human Untagged Clone

SKU SC109336

RNF170 (untagged)-Human ring finger protein 170 (RNF170), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: RNF170
• Synonyms: ADSA; SNAX1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_030954, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCAAATATCAAGGTGAAGTTCAAAGTTTGAAACTGGATGATGATTCAGTTATAGAAGGAGTAAGCG; ACCAAGTACTTGTGGCAGTTGTGGTCAGTTTCGCTTTGATTGCTACCCTGGTATATGCACTTTTCAGAAA; TGTACATCAAAACATTCACCCAGAAAACCAGGAGCTAGTAAGGGTACTTCGAGAACAGCTTCAAACAGAA; CAGGATGCACCTGCTGCCACTCGACAGCAGTTCTACACTGACATGTACTGTCCCATCTGCCTGCACCAAG; CCTCCTTCCCGGTGGAGACCAACTGTGGACATCTTTTTTGTGGTGCCTGCATTATTGCTTACTGGCGATA; TGGTTCATGGCTTGGGGCAATCAGTTGTCCAATCTGTAGACAAACGGTAACCTTACTCCTAACAGTATTT; GGTGAAGATGATCAGTCTCAGGATGTTCTGAGATTGCATCAGGATATTAATGATTATAACCGGAGATTCT; CAGGGCAACCCAGATCTATTATGGAGAGAATTATGGATCTACCCACTTTACTGAGGCATGCATTCAGGGA; AATGTTTTCAGTCGGGGGCCTTTTCTGGATGTTTCGCATCAGGATAATACTTTGTTTAATGGGAGCTTTT; TTCTATCTTATATCACCTCTAGATTTTGTACCTGAAGCCTTGTTTGGAATTCTAGGCTTTCTAGATGATT; TCTTTGTCATCTTTTTATTGCTTATCTACATCTCTATTATGTATCGAGAAGTGATAACCCAAAGGCTAAC; TAGATGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030954 unedited; TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGGGGTGGCGGGA; GCGGTCCTGGAAATAATCTGTCCTCTGTCGCCGGGAACTGGCGAGGTAGTTCCTTCGCGG; TGGAGAGACCTGGAATGGCCAAATATCAAGGTGAAGTTCAAAGTTTGAAACTGGATGATG; ATTCAGTTATAGAAGGAGTAAGCGACCAAGTACTTGTGGCAGTTGTGGTCAGTTTCGCTT; TGATTGCTACCCTGGTATATGCACTTTTCAGAAATGTACATCAAAACATTCACCCAGAAA; ACCAGGAGCTAGTAAGGGTACTTCGAGAACAGCTTCAAACAGAACAGGATGCACCTGCTG; CCACTCGACAGCAGTTCTACACTGACATGTACTGTCCCATCTGCCTGCACCAAGCCTCCT; TCCCGGTGGAGACCAACTGTGGACATCTTTTTTGTGGTGCCTGCATTATTGCTTACTGGC; GATATGGTTCATGGCTTGGGGCAATCAGTTGTCCAATCTGTAGACAAACGGTAACCTTAC; TCCTAACAGTATTTGGTGAAGATGATCAGTCTCAGGATGTTCTGAGATTGCATCAGGATA; TTAATGATTATAACCGGAGATTCTCAGGGCAACCCAGATCTATTATGGAGAGAATTATGG; ATCTACCCACTNTACTGAGGCATGCATTCANGAANATGTTNTCAGTCGGGGGCCTTTTCT; GGATGTTTCGCATCAGGATAATACTTTGTTTAATGGGAGCTTTTTTCTATCTTATATCAC; CTCTAGATTNTGTACCTGAAGCCCTGTTTGGAATNCTAGGCTTTCTAGATGAATTCTTTG; GCATCTTTTTATTGGCTATCTACATCTCTATTATGTATCGAGAAGTGATACCCCAAGGGC; TACTAGATC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030954 unedited; GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGAGGGAGTCTCACTCTGTTG; CCCAGGCTGGAGTGCAGTGGCGCAATCCACCTCCTGGGTTTACGCCATTCTTCTGCCTCA; GCCTCCCGAGTAGCTGGGACTACAGGCGCCTGCCACCACGCCTGGCTAATTTTTTGTATT; TTTAGTAGAGACGGGGTTTCACCATGTTAGCCAGGATGGTCCTGATCTCCTGACCTTGTG; ATCTACCCGCCTCAGCCTCCCAAAGTGCTAGGATTACAGGCGTGAGCCACCCCGCCCGGC; CATGTTTTTCATCTTAATTCTTCTATTGATTAAAATGTGTTCTGTAAACTAGAATATGAT; ATCGAAGTATGAAGTTCAAGTTTCTTCTTTCAGTTTCAGCTGATAGCAAATAATTAATTA; TACCAGAATGAAAAGGCAGAACTGCTCCAAATGCACAAAACTTAGCTAGCACTAAAAGAA; ATACTGAATTTTCCCCAATAGTATATAATATTTTATTGGAACAAAAATATTTAGATGAGT; TTCACAGCTATATATTATCATATAGGTACCTGCTGAGAAGGATAAGATGGATAAACTGAC; ATTTAACAATATTTTACTATACATCTAATTAATCTAAGTAATTCTGGGGAAAAGAAAATA; TATAAAAGCATCCCTTTTTATATAATTCCATATTTTTTCCAGACAACATAGAATCAAGAT; ACCACTGGTAGATCATTATAATTCTAAACTTAATATTANGAACTTCAAACATGAAAATTC; CAGTTATACCTAGGTATTTGTCAATGATATCAAATGGTGTCTTATAGGGTTTATATTGNG; AGAAATACTCACTGCTCATTGCTTTACTGCATGGNCCTCTGNNTGATGTCACATACCTCA; AAATCTATAACTTCAATTGTTTTTTTTATTANTACCTTTGGTTTACTTTCCACAAAAAGA; AGGAAAGCCTAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_030954
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030954.2, NP_112216.2
• RefSeq Size: 1851 bp
• RefSeq ORF: 777 bp
• Locus ID: 81790
• UniProt ID: Q96K19
• Cytogenetics: 8p11.21
• Domains: RING
• Protein Families: Druggable Genome, Transmembrane
• Summary: This gene encodes a RING domain-containing protein that resides in the endoplasmic reticulum (ER) membrane. This protein functions as an E3 ubiquitin ligase and mediates ubiquitination and processing of inositol 1,4,5-trisphosphate (IP3) receptors via the ER-associated protein degradation pathway. It is recruited to the activated IP3 receptors by the ERLIN1/ERLIN2 complex to which it is constitutively bound. Mutations in this gene are associated with autosomal dominant sensory ataxia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2012]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.