hnRNP K (HNRNPK) (NM_031263) Human Untagged Clone

SKU SC109316

HNRNPK (untagged)-Human heterogeneous nuclear ribonucleoprotein K (HNRNPK), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: hnRNP K
• Synonyms: AUKS; CSBP; HNRPK; TUNP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109316 sequence for NM_031263 edited (data generated by NextGen Sequencing); ATGGAAACTGAACAGCCAGAAGAAACCTTCCCTAACACTGAAACCAATGGTGAATTTGGT; AAACGCCCTGCAGAAGATATGGAAGAGGAACAAGCATTTAAAAGATCTAGAAACACTGAT; GAGATGGTTGAATTACGCATTCTGCTTCAGAGCAAGAATGCTGGGGCAGTGATTGGAAAA; GGAGGCAAGAATATTAAGGCTCTCCGTACAGACTACAATGCCAGTGTTTCAGTCCCAGAC; AGCAGTGGCCCCGAGCGCATATTGAGTATCAGTGCTGATATTGAAACAATTGGAGAAATT; CTGAAGAAAATCATCCCTACCTTGGAAGAGGGCCTGCAGTTGCCATCACCCACTGCAACC; AGCCAGCTCCCGCTCGAATCTGATGCTGTGGAATGCTTAAATTACCAACACTATAAAGGA; AGTGACTTTGACTGCGAGTTGAGGCTGTTGATTCATCAGAGTCTAGCAGGAGGAATTATT; GGGGTCAAAGGTGCTAAAATCAAAGAACTTCGAGAGAACACTCAAACCACCATCAAGCTT; TTCCAGGAATGCTGTCCTCATTCCACTGACAGAGTTGTTCTTATTGGAGGAAAACCCGAT; AGGGTTGTAGAGTGCATAAAGATCATCCTTGATCTTATATCTGAGTCTCCCATCAAAGGA; CGTGCACAGCCTTATGATCCCAATTTTTACGATGAAACCTATGATTATGGTGGTTTTACA; ATGATGTTTGATGACCGTCGCGGACGCCCAGTGGGATTTCCCATGCGGGGAAGAGGTGGT; TTTGACAGAATGCCTCCTGGTCGGGGTGGGCGTCCCATGCCTCCATCTAGAAGAGATTAT; GATGATATGAGCCCTCGTCGAGGACCACCTCCCCCTCCTCCCGGACGAGGCGGCCGGGGT; GGTAGCAGAGCTCGGAATCTTCCTCTTCCTCCACCACCACCACCTAGAGGGGGAGACCTC; ATGGCCTATGACAGAAGAGGGAGACCTGGAGACCGTTACGACGGCATGGTTGGTTTCAGT; GCTGATGAAACTTGGGACTCTGCAATAGATACATGGAGCCCATCAGAATGGCAGATGGCT; TATGAACCACAGGGTGGCTCCGGATATGATTATTCCTATGCAGGGGGTCGTGGCTCATAT; GGTGATCTTGGTGGACCTATTATTACTACACAAGTAACTATTCCCAAAGATTTGGCTGGA; TCTATTATTGGCAAAGGTGGTCAGCGGATTAAACAAATCCGTCATGAGTCGGGAGCTTCG; ATCAAAATTGATGAGCCTTTAGAAGGATCCGAAGATCGGATCATTACCATTACAGGAACA; CAGGACCAGATACAGAATGCACAGTATTTGCTGCAGAACAGTGTGAAGCAGTATGCAGAT; GTTGAAGGATTCTAA; ; Clone variation with respect to NM_031263.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031263 unedited; AATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTGAGGCGGCG; CGGCAGCGGAGGGACGGCAGTCTCGCGCGGCTACTGCAGCACTGGGGTGTCAGTTGTTGG; TCCGACCCAGAACGCTTCAGTTCTGCTCTGCAAGGATATATAATAACTGATTGGTGTGCC; CGTTTAATAAAAGAATATGGAAACTGAACAGCCAGAAGAAACCTTCCCTAACACTGAAAC; CAATGGTGAATTTGGTAAACGCCCTGCAGAAGATATGGAAGAGGAACAAGCATTTAAAAG; ATCTAGAAACACTGATGAGATGGTTGAATTACGCATTCTGCTTCAGAGCAAGAATGCTGG; GGCAGTGATTGGAAAAGGAGGCAAGAATATTAAGGCTCTCCGTACAGACTACAATGCCAG; TGTTTCAGTCCCAGACAGCAGTGGCCCCGAGCGCATATTGAGTATCAGTGCTGATATTGA; AACAATTGGAGAAATTCTGAAGAAAATCATCCCTACCTTGGAAGAGGGCCTGCAGTTGCC; ATCACCCACTGCAACCAGCCAGCTCCCGCTCGAATCTGATGCTGTGGAATGCTTAAATTA; CCAACACTATAAAGGAAGTGACTTTGACTGCGAGTTGAGGCTGTTGATTCATCAGAGTCT; AGCAGGAGGAATTATTGGGGTCAAAGGTGCTAAAATCAAAGAACTTCGAGAGAACACTCA; AACCACCTTCAAGCTTTTCCAGGAAACCTGTCCTCATTCCACTGACAGAGTTGTTCTTAT; TGAAGGACACCCGAATAGGTTGAAAAGTGCATAAAAACATCCTTGATCTATATCTGAGTC; TCCCATCAAAGGACTGCCCCACCTTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031263 unedited; TTGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTACATAAGATTACTA; TATTTCCTTTTAAGAGGAACGTTTCAACAAATTTTACAGGTGTCAATCAACCCTTGTTCA; AATCGGGCACACATCAAATCTAGCATCATGGGTGTTTTATTTATTTGGAAGTGAATTTTA; ACTATCAATACAAATGCCAAGATACTACACAAAACATCCACAGGAACTTTTTTCATCTTT; TTTTTTTTTGAATTGTACACAAACATTTCCTACATAATCCAACATACAACAGAGAAATGG; TACATCTTTTTCTTTCAATTTGCATACAATGGAAAAACAAGTATATATATATTTTACAAA; GTTTAACTAATAAGACACTAGAGCAAATTGACAGTTTAAGTCTATAGGTGAGAAATTATC; TAATAAAAATAATCAATTTTTTCAGCATTAGTCACTTCCATAACCAAGTGTTATTCTGAT; TAATAAAACTTGCTGCCAATCAGAATTCTGGTATATACTCATACCATTACTCAACTTGTA; GTACTGCTCCCCACCTTAGTTCTTCACAACTAACATAGAAAATTGTTGAAAAGTAGGGGC; AAGCATTTGCAAAAACAAACAAAAAATCCCCAGCTTATTATAAGCATGAATATGTATGAT; GGAATTTCTTCCCAGCAATAGACTTCCAAACCATCAAGAAATCACCAGAACTAAGTTTGC; CAAGTTATTTTGCCTATGTCNCAACAGAGATGCACTTATATGTCCAACAGAGGAGCTGAA; ATTAAACTTAGTGTTAGTTGGNGGTGGNGGTTGGGAATATCAANCCCCATTTTAAAATGA; CTGTCTTAAAAAAAATGACCACCAAAATAGGTCACTAAATTTATTTAAAATCATAAACGT; TCCTACAAAGACATTACATCTTGACCTGCTTGGACAGATGCAGGAACTGGGACTATGTTA; CTTTCCTCCTGTCCACCCCAATGTACAGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_031263
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_031263.1, NP_112553.1
• RefSeq Size: 2745 bp
• RefSeq ORF: 1395 bp
• Locus ID: 3190
• UniProt ID: P61978
• Cytogenetics: 9q21.32
• Domains: KH
• Protein Pathways: Spliceosome
• Summary: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) uses an alternate 5' non-coding exon, hence has a different 5' UTR compared to transcript variant 1. Transcript variants 1 and 2 encode the same isoform (a).