KAZALD1 (NM_030929) Human Untagged Clone

SKU SC109213

KAZALD1 (untagged)-Human Kazal-type serine peptidase inhibitor domain 1 (KAZALD1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: KAZALD1
• Synonyms: BONO1; FKSG28; FKSG40; IGFBP-rP10
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_030929, the custom clone sequence may differ by one or more nucleotides; ; ; ATGCTGCCGCCGCCGCGGCCCGCAGCTGCCTTGGCGCTGCCTGTGCTCCTGCTACTGCTGGTGGTGCTGA; CGCCGCCCCCGACCGGCGCAAGGCCATCCCCAGGCCCAGATTACCTGCGGCGCGGCTGGATGCGGCTGCT; AGCGGAGGGCGAGGGCTGCGCTCCCTGCCGGCCAGAAGAGTGCGCCGCGCCGCGGGGCTGCCTGGCGGGC; AGGGTGCGCGACGCGTGCGGCTGCTGCTGGGAATGCGCCAACCTCGAGGGCCAGCTCTGCGACCTGGACC; CCAGTGCTCACTTCTACGGGCACTGCGGCGAGCAGCTTGAGTGCCGGCTGGACACAGGCGGCGACCTGAG; CCGCGGAGAGGTGCCGGAACCTCTGTGTGCCTGTCGTTCGCAGAGTCCGCTCTGCGGGTCCGACGGTCAC; ACCTACTCCCAGATCTGCCGCCTGCAGGAGGCGGCCCGCGCTCGGCCCGATGCCAACCTCACTGTGGCAC; ACCCGGGGCCCTGCGAATCGGGGCCCCAGATCGTGTCACATCCATATGACACTTGGAATGTGACAGGGCA; GGATGTGATCTTTGGCTGTGAAGTGTTTGCCTACCCCATGGCCTCCATCGAGTGGAGGAAGGATGGCTTG; GACATCCAGCTGCCAGGGGATGACCCCCACATCTCTGTGCAGTTTAGGGGTGGACCCCAGAGGTTTGAGG; TGACTGGCTGGCTGCAGATCCAGGCTGTGCGTCCCAGTGATGAGGGCACTTACCGCTGCCTTGGCCGCAA; TGCCCTGGGTCAAGTGGAGGCCCCTGCTAGCTTGACAGTGCTCACACCTGACCAGCTGAACTCTACAGGC; ATCCCCCAGCTGCGATCACTAAACCTGGTTCCTGAGGAGGAGGCTGAGAGTGAAGAGAATGACGATTACT; ACTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030929 unedited; GGCGTTCAGAATTGTAAACGACTTATATAGGCGGCCGCGCTGCTGCGAAGGTCTAGTTCC; GGACACTAGGGTGCCCGAACGCGCTGATGCCCCGAGTGCTCGCAGGGCTTCCCGCTAACC; ATGCTGCCGCCGCCGCGGCCCGCAGCTGCCTTGGCGCTGCCTGTGCTCCTGCTACTGCTG; GTGGTGCTGACGCCGCCCCCGACCGGCGCAAGGCCATCCCCAGGCCCAGATTACCTGCGG; CGCGGCTGGATGCGGCTGCTAGCGGAGGGCGAGGGCTGCGCTCCCTGCCGGCCAGAAGAG; TGCGCCGCGCCGCGGGGCTGCCTGGCGGGCAGGGTGCGCGACGCGTGCGGCTGCTGCTGG; GAATGCGCCAACCTCGAGGGCCAGCTCTGCGACCTGGACCCCAGTGCTCACTTCTACGGG; CACTGCGGCGAGCAGCTTGAGTGCCGGCTGGACACAGGCGGCGACCTGAGCCGCGGAGAG; GTGCCGGAACCTCTGTGTGCCTGTCGTTCGCAGAGTCCGCTCTGCGGGTCCGACGGTCAC; ACCTACTCCCAGATCTGCCGCCTGCAGGAGGCGGCCCGCGCTCGGCCCGATGCCAACCTC; ACTGTGGCACACCCGGNGCCCTGCGAATCGGGGCCCCAGATCGTGTCACATCCATATGAC; ACTTGGAATGTGACAGGGCANGATGTGATCTTTGGCTGTGAAGTGTTTGCCTACCCCATG; GCCTCCATCGAGTGGAGGAAAGATGGCTTGGACATCCAGCTGCCAGGGGATGACCCCCAC; ATCTCTGTGCAGTTTAGGGGTGGACCCCANNAGTTTGAGGTGACTGGCTGGCTGCAAATN; CCAGCTGTGCGTCCCAGTGATGAAGGCACTTACCGCTGNCTTTGCCCGCATGCCCTGNGT; CAAGTGGAGCCCCTGCTAGCTGACAGTGCTCCN;
• Restriction Sites: NotI-NotI
• ACCN: NM_030929
• Insert Size: 1150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030929.3, NP_112191.2
• RefSeq Size: 1933 bp
• RefSeq ORF: 915 bp
• Locus ID: 81621
• UniProt ID: Q96I82
• Cytogenetics: 10q24.31
• Domains: ig, IG, IGc2, kazal
• Protein Families: Secreted Protein
• Summary: This gene encodes a secreted member of the insulin growth factor-binding protein (IGFBP) superfamily. The protein contains an insulin growth factor-binding domain in its N-terminal region, a Kazal-type serine protease inhibitor and follistatin-like domain in its central region, and an immunoglobulin-like domain in its C-terminal region. Studies of the mouse ortholog suggest that this protein may function in bone development and bone regeneration. This gene is hypomethylated and over-expressed in high-grade glioma compared to low-grade glioma, and thus the hypomethylated gene may be associated with cell proliferation and the shorter survival of patients with high-grade glioma. It is also one of numerous genes found to be deleted in a novel 5.54 Mb interstitial deletion, which is associated with multiple congenital anomalies. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]; Transcript Variant: This variant (1) encodes the longer isoform (a).