EDG2 (LPAR1) (NM_057159) Human Untagged Clone

SKU SC109187

LPAR1 (untagged)-Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: EDG2
• Synonyms: edg-2; EDG2; Gpcr26; LPA1; Mrec1.3; rec.1.3; vzg-1; VZG1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109187 sequence for NM_057159 edited (data generated by NextGen Sequencing); ATGGCTGCCATCTCTACTTCCATCCCTGTAATTTCACAGCCCCAGTTCACAGCCATGAAT; GAACCACAGTGCTTCTACAACGAGTCCATTGCCTTCTTTTATAACCGAAGTGGAAAGCAT; CTTGCCACAGAATGGAACACAGTCAGCAAGCTGGTGATGGGACTTGGAATCACTGTTTGT; ATCTTCATCATGTTGGCCAACCTATTGGTCATGGTGGCAATCTATGTCAACCGCCGCTTC; CATTTTCCTATTTATTACCTAATGGCTAATCTGGCTGCTGCAGACTTCTTTGCTGGGTTG; GCCTACTTCTATCTCATGTTCAACACAGGACCCAATACTCGGAGACTGACTGTTAGCACA; TGGCTCCTTCGTCAGGGCCTCATTGACACCAGCCTGACGGCATCTGTGGCCAACTTACTG; GCTATTGCAATCGAGAGGCACATTACGGTTTTCCGCATGCAGCTCCACACACGGATGAGC; AACCGGCGGGTAGTGGTGGTCATTGTGGTCATCTGGACTATGGCCATCGTTATGGGTGCT; ATACCCAGTGTGGGCTGGAACTGTATCTGTGATATTGAAAATTGTTCCAACATGGCACCC; CTCTACAGTGACTCTTACTTAGTCTTCTGGGCCATTTTCAACTTGGTGACCTTTGTGGTA; ATGGTGGTTCTCTATGCTCACATCTTTGGCTATGTTCGCCAGAGGACTATGAGAATGTCT; CGGCATAGTTCTGGACCCCGGCGGAATCGGGATACCATGATGAGTCTTCTGAAGACTGTG; GTCATTGTGCTTGGGGCCTTTATCATCTGCTGGACTCCTGGATTGGTTTTGTTACTTCTA; GACGTGTGCTGTCCACAGTGCGACGTGCTGGCCTATGAGAAATTCTTCCTTCTCCTTGCT; GAATTCAACTCTGCCATGAACCCCATCATTTACTCCTACCGCGACAAAGAAATGAGCGCC; ACCTTTAGGCAGATCCTCTGCTGCCAGCGCAGTGAGAACCCCACCGGCCCCACAGAAGGC; TCAGACCGCTCGGCTTCCTCCCTCAACCACACCATCTTGGCTGGAGTTCACAGCAATGAC; CACTCTGTGGTTTAG; ; Clone variation with respect to NM_057159.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_057159 unedited; GCGTTAGGATTTTGTAATACGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGGCACA; CTGTCCCGCCGCGGACGGGCTTTGTGGTTGGGGGCGCGCGTGCGAGTGCCAGTGAGAGTG; TGGGTGCGCGCTGTGGGCCGCGGCGCGGGTGGGTGGCCGTGCGTTCTTGCGAGCCGGCCT; GCAGGAGGCGAGGCTCCCCTGGCCTCCCGCACCCAGCGGCGGACCGAGCCCCTGGAGGGA; AGTTGCCGCAGCCGCCCGGGCCGCCGGCCCTCCTGTCCCGCGCCAGGGAACAGCTCCTGC; CCAGGTCTGTGGGCACTCAGCATGGATTTCAGTCTCCCTGTGAGTGATGGGAAAGAACTA; ACAGAGGCTGCAAGGTTTTACTGAATCTATTCCATTAGTGACCTACTGGAACGTTCAAAG; AATAAAAATCTCACTTGCTCAGTGTTTTTGATACACAGTGACATTCAGTCTGAAGTGTCA; TCCTTGAAGGATAGAATAAATCAGCTGTCGTGTTCTCAGTGAAAAGTGGAAAGAAGTTTT; AAAGGATCTGCANAATAGAATTATGAGACAGAGGAAAGAAAGAAACAGACAATCTGATCT; CTATCTTCATCGGCCAGGTGGACGTCTGATTTATGAAGCTCCCCATCCACCTATCTGAGT; ACCTGACTTCTCAGGACTGACACCTACAGCATCAGGTACACAGCTTCTCCTAGCATGACT; TCGATCTGATCACGCAACAAGAAAATTTGTCTTCCCGTATTTTTGGGGCGTGTTCACCAC; CTACAACCACCGGAGCTGTCATGGGTTGCCATTTCTACTTCCATCCCTGTGATTTTCACA; GCCCCCGGTTCACAGCCATGAATGAAACCACATGCTTTTAAAACGAGTCCATTGCGCTCT; TTTTATAACCCAGTGGGAAGTATT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_057159 unedited; NNNTTTACACTTTGGAACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTT; GATTGAAAATGATTTATTAAAGTCCAATTAGTATGCTTTTCATTTCAAATAATCCATATA; GCCTCCAGAAAAATATGCACATGTGTAAAAGTCCACGTTCATTTCTTTCACTTCCAATAT; AAAGTATTCTGTATTTTGTATAAAGTACGTGCAAACACCTTTCTGCTAATCGGGTCCCCA; CATTCTTTTCACTACAGGTACTTTACAAGTCTGCCCTCTGCTCAAACACTAACCGTGCAC; TGACATCCTCCTTCCTAGACAGCCATTCATCTCCCGGACTTCTTTCTCTCAGACATCCTC; CTGACCTCCCCTGACCTGCTTCACCACTGTGTTACCTCACTGGTTACTTGTTACAGCAAA; CTGATGCAACTACTAGTCTACCTGGACAACATATTAAACAGGTATCACCTAATAGGGTGG; CAGCCTATCGGGGTGATTCCTGGCGAATACACAGTAACCAACCACATACTGACACACTCA; ACCCATTTGCTACAGATGGACCCACACTAATTGATATGACAATCCTTTATTCACTCGGCA; CATTTGGTTTCTTTGCATTTTCTTCCATTTTACATTGCAGGTGTGGCTACCAAGAGCTGG; ATAACGAGTCCCTCAAACAAAGTTTGGAATTGCGAGATAAATTGGGGTACCTTGATTCTT; GAGACAGTTGAATTCCTCTAGTTGATCTTGGTGTATTAAAAAGTCCCTCTCAACTGAAGT; GAACACTGCATTTCTTTTGGAAAAAAGTCAATTGACTGGCTTTTCCTTACAAATGCCCCC; CTTGCCGTACAAAGCCGGGAATCCCTTTTATGGAAGGGGTAATTCCAAGAAGACT;
• Restriction Sites: NotI-NotI
• ACCN: NM_057159
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_057159.2, NP_476500.1
• RefSeq Size: 3182 bp
• RefSeq ORF: 1095 bp
• Locus ID: 1902
• UniProt ID: Q92633
• Cytogenetics: 9q31.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Gap junction, Neuroactive ligand-receptor interaction
• Summary: The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Many transcript variants encoding a few different isoforms have been identified for this gene. [provided by RefSeq, Oct 2020]; Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-26 encode the same protein.