CCR2 (NM_000648) Human Untagged Clone

CAT#: SC109082

CCR2 (untagged)-Human chemokine (C-C motif) receptor 2 (CCR2), transcript variant B

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCR2
• Synonyms: CC-CKR-2; CCR2A; CCR2B; CD192; CKR2; CKR2A; CKR2B; CMKBR2; MCP-1-R
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109082 sequence for NM_000648 edited (data generated by NextGen Sequencing)
  ATGCTGTCCACATCTCGTTCTCGGTTTATCAGAAATACCAACGAGAGCGGTGAAGAAGTC
  ACCACCTTTTTTGATTATGATTACGGTGCTCCCTGTCATAAATTTGACGTGAAGCAAATT
  GGGGCCCAACTCCTGCCTCCGCTCTACTCGCTGGTGTTCATCTTTGGTTTTGTGGGCAAC
  ATGCTGGTCATCCTCATCTTAATAAACTGCAAAAAGCTGAAGTGCTTGACTGACATTTAC
  CTGCTCAACCTGGCCATCTCTGATCTGCTTTTTCTTATTACTCTCCCATTGTGGGCTCAC
  TCTGCTGCAAATGAGTGGGTCTTTGGGAATGCAATGTGCAAATTATTCACAGGGCTGTAT
  CACATCGGTTATTTTGGCGGAATCTTCTTCATCATCCTCCTGACAATCGATAGATACCTG
  GCTATTGTCCATGCTGTGTTTGCTTTAAAAGCCAGGACGGTCACCTTTGGGGTGGTGACA
  AGTGTGATCACCTGGTTGGTGGCTGTGTTTGCTTCTGTCCCAGGAATCATCTTTACTAAA
  TGCCAGAAAGAAGATTCTGTTTATGTCTGTGGCCCTTATTTTCCACGAGGATGGAATAAT
  TTCCACACAATAATGAGGAACATTTTGGGGCTGGTCCTGCCGCTGCTCATCATGGTCATC
  TGCTACTCGGGAATCCTGAAAACCCTGCTTCGGTGTCGAAACGAGAAGAAGAGGCATAGG
  GCAGTGAGAGTCATCTTCACCATCATGATTGTTTACTTTCTCTTCTGGACTCCCTATAAT
  ATTGTCATTCTCCTGAACACCTTCCAGGAATTCTTCGGCCTGAGTAACTGTGAAAGCACC
  AGTCAACTGGACCAAGCCACGCAGGTGACAGAGACTCTTGGGATGACTCACTGCTGCATC
  AATCCCATCATCTATGCCTTCGTTGGGGAGAAGTTCAGAAGGTATCTCTCGGTGTTCTTC
  CGAAAGCACATCACCAAGCGCTTCTGCAAACAATGTCCAGTTTTCTACAGGGAGACAGTG
  GATGGAGTGACTTCAACAAACACGCCTTCCACTGGGGAGCAGGAAGTCTCGGCTGGTTTA
  TAA
  
  Clone variation with respect to NM_000648.2
  190 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000648 unedited
  GGGGGGGACCNNNNNCCCTTTCCCNCCCGGTTCAGATTTGTNATACGACTCACTATAGGC
  GGCCGCGAATTCGCACGAGGCTGAACAGAGAAGTGGATTGAACAAGGACGCATTTCCCCA
  GTACATCCACAACATGCTGTCCACATCTCGTTCTCGGTTTATCAGAAATACCAACGAGAG
  CGGTGAAGAAGTCACCACCTTTTTTGATTATGATTACGGTGCTCCCTGTCATAAATTTGA
  CGTGAAGCAAATTGGGGCCCAACTCCTGCCTCCGCTCTACTCGCTGGTGTTCATCTTTGG
  TTTTGTGGGCAACATGCTGGTCATCCTCATCTTAATAAACTGCAAAAAGCTGAAGTGCTT
  GACTGACATTTACCTGCTCAACCTGGCCATCTCTGATCTGCTTTTTCTTATTACTCTCCC
  ATTGTGGGCTCACTCTGCTGCAAATGAGTGGGTCTTTGGGAATGCAATGTGCAAATTATT
  CACAGGGCTGTATCACATCGGTTATTTTGGCGGAATCTTCTTCATCATCCTCCTGACAAT
  CGATAGATACCTGGCTATTGTCCATGCTGTGTTTGCTTTAAAAGCCAGGACGGTCACCTT
  TGGGGTGGTGACAAGTGTGATCACCTGGTTGGTGGCTGTNGTTGCTTCTGTCCCCAGAAT
  CATCTTTACTAAATGCCAGANAGAAGATTCTGTTTATGTCTGTGGCCCTTTATTTCCACG
  AGGATGGAAATATTNCACANCATTATGAGGAACATTNNTGNGCTGGTCCCTGCGCTGCTC
  ATCATGGNCATCTGCTACTCGGNAATCCTGAAACCCTGCTTNCGTGTCGAAACGAGAAGA
  AGAGCATAGGGCAGTGAGAGTCATCTTCACATCATNGATGTTACTTTCTCTTCTGGACTC
  CCTATATATGNCATCTNNCTGACACTTNCAGNNATCTTCGNCTGAGTACTGTGAAGCACA
  GTCACTGGACCAGCCGCAGTGACAGAACTCTGGGAGACTACTGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000648 unedited
  CTTTGGCCCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCTTTTATCAA
  CATAGTTATAGCATTGATTTATTTTCCAAATCATAGTTGTTAAAAACAGTATTTACATTT
  CATCACCATCAACTTAACATTTTATGCATTCATTAGGATCATACAAATATATGAAGGTGA
  AAGTTCTGCTCTGTCCCCACTTCTTTCTGAAACCTTCTTTAGCCATGTGGCCTGAAAGTA
  GAGCCATAGTCCAGCTTGGGAGTCTAGTTCCCAAGTTGCCTGGTTCTAGAAGCCCAGTGT
  TTCTTGCTTGCATCAGATCAGAAACACACTGGGCAACATAAGAACATTCTTAACTTGGCT
  CGACTAAGAGGAAAGTCTTTGCTTACTTCAGGGAGCTTCTCCACCTTCCATTCTTTCCCA
  ATAGGGTTTAAAAGCTTTGATTAGAAGCCAACTTGATTTAGGAGTTCCCACTGGCAAAAT
  AGGGAACAATATGAGCATCAAGGACATCTGCGAAGCACTGAAACACTCGAATGTGATTAA
  ACGCATGACTTCATAATGATACAAAACAAAACACAAAAGGCTTTTGGCAGCAAGCCTGAG
  AGAATGGAGATCCAGCTGAGATGACAGCGATGGAGCGTATACTGTGATTCAAACGACATC
  ATTACTTAGATGCTGCATGCACATAGGCTAATGTGCCCACAGATCACACACTTCCAACTC
  CTGCCAATCAACAACTATTGGGGTTGACTCAGCAGTCTCCCAAACATACCACCTGCTCAA
  AATTCTTAGCTCAAGCTAGATTATCTTGTGACTAATACAGATTTCACGTTTGACTGATAG
  TACTCAACTAACTCTTACACATTACAAGTGCAGTTTCAGCTTAATTTATTTTACTATTAA
  AAAAGCTGACTCTATGCTGTATCAAAAGAGGACTNAAGTAGTTGGAAAACTGTTCCACCC
  ACTGGAGCCTCTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000648
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000648.1, NP_000639.1
• RefSeq Size: 1979 bp
• RefSeq ORF: 1083 bp
• Locus ID: 1231
• Cytogenetics: 3p21.31
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene. [provided by RefSeq]
  Transcript Variant: This variant (B) lacks the 3' exon but has an alternate 3' segment, as compared to transcript A. It encodes the predominant isoform (B) which localizes to the plasma membrane. Isoform B has a distinct C-terminus and is 14 aa shorter than isoform A.