alpha 1a Adrenergic Receptor (ADRA1A) (NM_000680) Human Untagged Clone

SKU SC108910

ADRA1A (untagged)-Human adrenergic, alpha-1A-, receptor (ADRA1A), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: alpha 1a Adrenergic Receptor
• Synonyms: ADRA1C; ADRA1L1; ALPHA1AAR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000680, RT-PCR generated; ATGGTGTTTCTCTCGGGAAATGCTTCCGACAGCTCCAACTGCACCCAACCGCCGGCACCG; GTGAACATTTCCAAGGCCATTCTGCTCGGGGTGATCTTGGGGGGCCTCATTCTTTTCGGG; GTGCTGGGTAACATCCTAGTGATCCTCTCCGTAGCCTGTCACCGACACCTGCACTCAGTC; ACGCACTACTACATCGTCAACCTGGCGGTGGCCGACCTCCTGCTCACCTCCACGGTGCTG; CCCTTCTCCGCCATCTTCGAGGTCCTAGGCTACTGGGCCTTCGGCAGGGTCTTCTGCAAC; ATCTGGGCGGCAGTGGATGTGCTGTGCTGCACCGCGTCCATCATGGGCCTCTGCATCATC; TCCATCGACCGCTACATCGGCGTGAGCTACCCGCTGCGCTACCCAACCATCGTCACCCAG; AGGAGGGGTCTCATGGCTCTGCTCTGCGTCTGGGCACTCTCCCTGGTCATATCCATTGGA; CCCCTGTTCGGCTGGAGGCAGCCGGCCCCCGAGGACGAGACCATCTGCCAGATCAACGAG; GAGCCGGGCTACGCGCTCTTCTCAGCGCTGGGCTCCTTCTACCTGCCTCTGGCCATCATC; CTGGTCATGTACTGCCGCGTCTACGTGGTGGCCAAGAGGGAGAGCCGGGGCCTCAAGTCT; GGCCTCAAGACCGACAAGTCGGACTCGGAGCAAGTGACGCTCCGCATCCATCGGAAAAAC; GCCCCGGCAGGAGGCAGCGGGATGGCCAGCGCCAAGACCAAGACGCACTTCTCAGTGAGG; CTCCTCAAGTTCTCCCGGGAGAAGAAAGCGGCCAAAACGCTGGGCATCGTGGTCGGCTGC; TTCGTCCTCTGCTGGCTGCCTTTTTTCTTAGTCATGCCCATTGGGTCTTTCTTCCCTGAT; TTCAAGCCCTCTGAAACAGTTTTTAAAATAGTATTTTGGCTCGGATATCTAAACAGCTGC; ATCAACCCCATCATATACCCATGCTCCAGCCAAGAGTTCAAAAAGGCCTTTCAGAATGTC; TTGAGAATCCAGTGTCTCCGCAGAAAGCAGTCTTCCAAACATGCCCTGGGCTACACCCTG; CACCCGCCCAGCCAGGCCGTGGAAGGGCAACACAAGGACATGGTGCGCATCCCCGTGGGA; TCAAGAGAGACCTTCTACAGGATCTCCAAGACGGATGGCGTTTGTGAATGGAAATTTTTC; TCTTCCATGCCCCGTGGATCTGCCAGGATTACAGTGTCCAAAGACCAATCCTCCTGTACC; ACAGCCCGGGTGAGAAGTAAAAGCTTTTTGCAGGTCTGCTGCTGTGTAGGGCCCTCAACC; CCCAGCCTTGACAAGAACCATCAAGTTCCAACCATTAAGGTCCACACCATCTCCCTCAGT; GAGAACGGGGAGGAAGTCTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000680 unedited; CCTTGGATTTGTAAACGACTTATATAGGCGGCCGCCACTGTGATGGGTATCTGCAGAATT; CGGCTTTGGGACCATGGTGTTTCTCTCGGGAAAGCTTCCGACAGCTCCAACTGCACCCAA; CCGCCGGCACCGGTGAACATTTCCAAGGCCATTCTGCTCGGGGTGATCTTGGGGGGCCTC; ATTCTTTTCGGGGTGCTGGGTAACATCCTATTGATCCTCTCCGTAGCCTGTCACCGACAC; CTGCACTCAGTCACGCACTACTACATCGTCAACCTGGCGGTGGCCGACCTCCTGCTCACC; TCCACGGTGCTGCCCTTCTCCGCCATCTTCGAGGTCCTAGGCTACTGGGCCTTCGGCAGG; GTCTTCTGCAACATCTGGGCGGCAGTGGATGTGCTGTGCTGCACCGCGTCCATCATGGGC; CTCTGCATCATCTCCATCGACCGCTACATCGGCGTGAGCTACCCGCTGCGCTACCCAACC; ATCGTCACCCAGAGGAGGGGTCTCATGGCTCTGCTCTGCGTCTGGGCACTCTCCCTGGTC; ATATCCATTGGACCCCTGTTCGGCTGGAGGCAGCCGGCCCCCGAGGACGAGACCATCTGC; CAGATCAACGAGGAGCCGGGCTACGCGCTCTTCTCAGCGCTGGGCTCCTTCTACCTGCCT; CTGGCCATCATCCTGGTCATGTACTGCCGCGTCTACGTGGTGGGCCAAGAGGAGAGCCGG; GGCCTCAAGTCTGGCCTCAGACCGACCAGNNTCGACTCGGAGCAGTGACGCTCCGCATCC; ATCGGAAAAACGCCCCGCAGGGAGCAGCGGGATGGCCAGCGCCNAGACCAGACGCACTTC; TCAGTGAGGCTCCCTCAGTTCTCCCGGGAGAAGAAAAGCGCCANACGCTGGCATCGTGGN; TCGCTGCTTCGTCCTCTGCTGGCTGGCTTTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000680 unedited; NCGACAANCTTGATATCGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCATGTGCTGG; AATTCGGCTTTCCTGTCCTAGACTTCCTCCCCGTTCTCACTGAGGGAGATGGTGTGGACC; TTAATGGTTGGAACTTGATGGTTCTTGTCAAGGCTGGGGGTTGAGGGCCCTACACAGCAG; CAGACCTGCAAAAAGCTTTTACTTCTCACCCGGGCTGTGGTACAGGAGGATTGGTCTTTG; GACACTGTAATCCTGGCAGATCCACGGGGCATGGAAGAGAAAAATTTCCATTCACAAACG; CCATCCGTCTTGGAGATCCTGTAGAAGGTCTCTCTTGATCCCACGGGGATGCGCACCATG; TCCTTGTGTTGCCCTTCCACGGCCTGGCTGGGCGGGTGCAGGGTGTAGCCCAGGGCATGT; TTGGAAGACTGCTTTCTGCGGAGACACTGGATTCTCAAGACATTCTGAAAGGCCTTTTTG; AACTCTTGGCTGGAGCATGGGTATATGATGGGGTTGATGCAGCTGTTTAGATATCCGAGC; CAAAATACTATTTTAAAAACTGTTTCAGAGGGCTTGATATCAGGGAAGAAAGACCCAATG; GGCATGACTAAGAAAAAAGGCAGCCAGCAGAGGACGAAGCAGCCGACCACGATGCCCAGC; GTTTTGGCCGCTTTCTTCTCCCGGGAGAACTTGANGAGCCTCACTGAGAAGTGCGTCTTG; GTCTTGGGCGCTGCCATCCNGCTGCCTNCTGCCGGGGCGTTTTTCCGATGGATGCGGAGC; GTCACTTGCTTCGAGTCCGACTTGTTCGTCTTGAGGCCAGACTTGAGGGCCCGGCTCTCC; CTCTTGGCACCACGTAAACGCGGCG;
• Restriction Sites: Please inquire
• ACCN: NM_000680
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000680.1, NP_000671.1
• RefSeq Size: 2290 bp
• RefSeq ORF: 1401 bp
• Locus ID: 148
• UniProt ID: P35348
• Cytogenetics: 8p21.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction
• Summary: Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) is alternatively spliced in the 3' end, compared to variant 3. It encodes isoform 1, which has a longer and distinct C-terminus compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.