VPS54 (NM_016516) Human Untagged Clone

SKU SC108738

VPS54 (untagged)-Human vacuolar protein sorting 54 homolog (S. cerevisiae) (VPS54), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: VPS54
• Synonyms: HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L; WR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108738 sequence for NM_016516 edited (data generated by NextGen Sequencing); ATGGCTTCAAGCCACAGTTCTTCACCAGTGCCTCAAGGAAGCAGCAGTGATGTTTTCTTT; AAAATAGAGGTAGATCCGTCAAAACACATTCGACCTGTGCCATCACTGCCAGATGTGTGT; CCCAAGGAACCCACAGGTGATTCACATAGTTTATATGTTGCCCCATCTCTAGTTACAGAT; CAACATAGATGGACTGTATATCATTCCAAAGTAAATCTCCCAGCAGCATTAAACGATCCT; AGATTAGCAAAAAGAGAATCTGACTTCTTCACAAAAACATGGGGATTGGACTTTGTGGAC; ACTGAAGTCATACCTTCATTCTACCTCCCACAGATCAGCAAGGAACATTTTACAGTATAT; CAACAGGAAATCTCTCAGAGAGAGAAGATTCATGAGAGATGCAAGAATATTTGTCCTCCT; AAAGATACCTTCGAAAGGACTCTTTTACATACTCATGATAAATCCAGGACAGATCTGGAG; CAAGTACCTAAGATTTTTATGAAACCAGATTTTGCCTTGGATGATTCCTTAACTTTTAAT; TCAGTTTTACCATGGTCTCATTTTAATACTGCTGGTGGAAAAGGAAATCGTGATGCAGCT; TCCTCAAAGTTGCTTCAAGAAAAGCTGAGCCATTATCTGGATATTGTGGAAGTAAACATT; GCTCACCAGATCTCTCTACGTTCAGAAGCATTTTTTCATGCAATGACCTCTCAACACGAG; TTGCAGGACTACCTCAGGAAAACTTCCCAGGCTGTAAAAATGCTTCGAGATAAAATTGCA; CAGATTGATAAAGTAATGTGTGAAGGATCACTCCACATTTTAAGACTGGCACTTACCAGA; AATAATTGTGTTAAAGTATACAATAAGCTGAAGTTAATGGCCACTGTACACCAGACTCAG; CCTACAGTACAGGTGTTATTATCTACTTCTGAATTTGTTGGAGCATTGGACTTAATAGCA; ACAACACAAGAGGTTCTACAGCAGGAACTTCAGGGCATTCACAGTTTCCGGCATTTGGGA; TCACAGCTTTGTGAATTAGAAAAACTGATAGATAAAATGATGATTGCAGAATTTTCTACT; TATTCTCACAGTGACTTAAATAGACCACTGGAAGATGACTGTCAAGTTTTAGAAGAGGAA; AGACTAATATCTCTTGTATTTGGACTTTTAAAACAAAGAAAGCTTAATTTTTTAGAAATC; TATGGTGAAAAAATGGTTATTACAGCAAAGAATATCATTAAACAGTGTGTGATTAATAAA; GTTTCACAAACAGAAGAAATAGACACAGATGTTGTTGTGAAGCTTGCAGATCAGATGAGA; ATGTTGAATTTTCCCCAGTGGTTTGATCTGCTCAAGGATATTTTCTCTAAGTTTACAATT; TTCCTACAGAGAGTGAAGGCAACATTAAATATCATTCACAGTGTTGTTCTCTCAGTTCTT; GACAAAAACCAAAGGACTAGAGAATTGGAAGAGATTTCACAACAGAAGAATGCTGCAAAA; GATAATTCACTGGACACAGAGGTGGCTTATTTAATCCATGAAGGCATGTTTATAAGTGAT; GCATTCGGTGAGGGTGAGCTAACACCTATAGCAGTTGACACTACCTCTCAAAGAAATGCA; TCTCCAAATAGTGAGCCCTGCAGCAGTGATTCTGTATCCGAGCCAGAATGTACTACTGAT; TCTTCATCCAGCAAAGAGCACACATCATCATCTGCTATTCCAGGAGGTGTGGATATTATG; GTCAGTGAAGATATGAAATTAACTGACTCAGAGCTAGGAAAGCTGGCAAATAATATCCAG; GAATTATTATATAGTGCCTCAGATATATGCCATGATCGAGCTGTCAAATTTCTCATGTCA; AGAGCAAAGGATGGTTTTCTTGAGAAGCTAAATTCCATGGAATTCATAACACTTTCTAGA; TTAATGGAAACATTCATTTTAGACACCGAACAGATCTGTGGAAGAAAAAGCACGTCATTA; CTTGGAGCACTTCAGAGCCAAGCTATTAAGTTTGTAAATAGGTTTCATGAAGAGAGAAAA; ACCAAGCTCAGCCTCCTCTTAGACAATGAGCGCTGGAAGCAAGCAGATGTTCCTGCAGAA; TTTCAGGATCTTGTTGATTCTCTGTCAGATGGGAAGATTGCTTTACCTGAAAAAAAATCA; GGAGCCACAGAAGAAAGGAAACCAGCTGAAGTTCTTATTGTCGAGGGACAACAGTATGCA; GTTGTTGGAACCGTATTGCTGTTAATAAGAATTATCCTTGAATATTGCCAGTGTGTGGAT; AACATCCCATCTGTTACTACTGACATGCTTACTCGTCTGTCAGATTTATTGAAGTACTTC; AATTCAAGAAGTTGCCAGTTAGTTCTTGGAGCTGGTGCACTGCAAGTTGTTGGACTAAAA; ACGATAACTACAAAAAATTTGGCTCTTTCTTCACGATGTTTGCAGTTAATTGTGCACTAC; ATTCCTGTGATCCGGGCTCATTTTGAAGCTCGACTACCACCTAAGCAATATAGCATGCTT; AGGCATTTTGATCATATCACTAAGGACTACCATGATCACATAGCTGAAATATCAGCTAAG; CTTGTAGCGATAATGGATAGCTTATTTGACAAGCTGTTATCTAAGTATGAAGTGAAGGCT; CCTGTTCCTTCTGCCTGTTTCAGGAATATTTGTAAGCAAATGACAAAAATGCACGAAGCT; ATATTTGATCTCCTTCCAGAAGAACAAACACAGATGTTATTTTTAAGAATTAATGCAAGT; TATAAACTCCACTTGAAAAAGCAGTTATCTCACTTAAATGTGATAAATGATGGAGGACCT; CAAAATGGGTTGGTCACAGCAGATGTAGCTTTTTACACTGGAAATCTTCAAGCCTTAAAA; GGCCTTAAAGATTTGGACCTAAATATGGCCGAAATTTGGGAGCAGAAGAGGTGA; ; Clone variation with respect to NM_016516.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016516 unedited; NGGTTTTCAGGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGGCCACC; TCTCACCCCTGAGTGAAGCTGGGCTCGAGAGGGCGGCCCTGTGCTCCCCGGGCCGACTGG; CCAGCGGGCGCGGGGCGGGGGCGGGAACCCGGGCTCGGGCCCGGCCGGGCGCCGGGCGGC; GGCGGCCGTGGAGCAGCAGCCTCGGTGCGACGTGGAGGGCTGGAGGCGGCGGCGATGCAC; TAGGCCTCGCTCAGGGCGGCTGCCCCGGGACCCGCAGTTGAGTGGTGATTTTATGCAATG; GCTTCAAGCCACAGTTCTTCACCAGTGCCTCAAGGAAGCAGCAGTGATGTTTTCTTTAAA; ATAGAGGTAGATCCGTCAAAACACATTCGACCTGTGCCATCACTGCCAGATGTGTGTCCC; AAGGAACCCACAGGTGATTCACATAGTTTATATGTTGCCCCATCTCTAGTTACAGATCAA; CATAGATGGACTGTATATCATTCCAAAGTAAATCTCCCAGCAGCATTAAACGATCCTAGA; TTAGCAAAAAGAGAATCTGACTTCTTCACAAAAACATGGGGATTGGACTTTGTGGACACT; GAAGTCATACCTTCATTCTACCTCCCACAGATCAGCAAGGAACATTTTACAGTATATCAA; CAGGAAATCTCTCAGAGAGAGAAGATTCATGAGAGATGCAAGAATATTTGTCCTCCTAAA; GATACCTTCGAAGGACTCTTTTCATACTCATGATAAATCCAGGACAGATCTGGAGCCAGT; ACCTAAGATTTTATGAAACCAGATTTGCCTCGGATGATCTCTTAACTTTAATNCAGTTTT; CCATGGNCTCATTTTATACTGCTGGNGGNAAAGGAATTCGGAGCAGCTCCTTAAGTTGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016516 unedited; CTTTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTAATTTT; TTACAGCTTTATTTTAGACAGATAGTTTAAGAACCAAAGACATACCTCTGTAATGATAAA; GGAAAGAAAACAAGCTTTCCTTTTAAGAAACCAAAGAGCACAAAATAAGACTGTTTCATT; ATACATAATCACCACAGGATATTAGGCACTCTGACAGGGTTAGGCAAGATTCTTGGTGTG; AGGTGAAGCACAGGCACTTTATTTGTACAGTGCTGCTGATTCTAATTTTGAAGGTAGGTA; TTATAAAAGTCTTTACTTGTCACCTTATTTCTGGCCCCAACACAGCAGCCTATAGTTTTA; AAAGTTCTGTTTCTCCCTGGTCTTTGTTCGTATACACATCGAAAGTAACTTAAAAACAAG; GATCCAAGGGGGCCATACTTCATATGTTATCTAAATGTTAATATGAGAACTCAAAAGTAG; GCAGATTATATGAATACATATTCTTACCTCTGCTACAAATAAAAACACCCCAAACCCTTC; ATCATACTTTTATAAAAATACAGATATTAAACTGTAGCCTTTTATATACCATTTCTGAAA; AGTATTTAGTTGCATAAATAGATGCCAGGATCTTTTTTTTTAAGTATTAATTACTTAAAA; AAAGGCTTAAGTCTTTCACGTATTTAGAGAGCTCTGGAAGGCTTTACCCAGTTGACATAC; CGTGGTAGTAAAAATATTAAATATGGCACACACAAGCCCTGCAACTTGACACTGGCTCAC; TGAAACAAACATTGAATTCCTAAATCCCAACCATCCAAAGATGTTTATTTTTTATAGAAA; GATCTGTAAACAAAATATAAATTTCTCAACCGCTTTTACTTTCATAGAAAAAACTTCTAC; AGAAGGTGACTAACACTTTAAATGTACCATCATTAATAGGGCGGGACACCTTTGAATTTC; TTGCCCTGAAAAATGCAAACATTTTAATTTAAATT;
• Restriction Sites: NotI-NotI
• ACCN: NM_016516
• Insert Size: 4640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016516.2, NP_057600.2
• RefSeq Size: 3994 bp
• RefSeq ORF: 2934 bp
• Locus ID: 51542
• UniProt ID: Q9P1Q0
• Cytogenetics: 2p15-p14
• Summary: This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer isoform (1).