SMPDL3B (NM_014474) Human Untagged Clone

SKU SC108594

SMPDL3B (untagged)-Human sphingomyelin phosphodiesterase, acid-like 3B (SMPDL3B), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SMPDL3B
• Synonyms: ASML3B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108594 sequence for NM_014474 edited (data generated by NextGen Sequencing); ATGAGGCTGCTCGCCTGGCTGATTTTCCTGGCTAACTGGGGAGGTGCCAGGGCTGAACCA; GGTAAGTTCTGGCACATCGCTGACCTGCACCTTGACCCTGACTACAAGGTATCCAAAGAC; CCCTTCCAGGTGTGCCCATCAGCTGGATCCCAGCCAGTGCCCGACGCAGGCCCCTGGGGT; GACTACCTCTGTGATTCTCCCTGGGCCCTCATCAACTCCTCCATCTATGCCATGAAGGAG; ATTGAGCCAGAGCCAGACTTCATTCTCTGGACTGGTGATGACACGCCTCATGTGCCCGAT; GAGAAACTGGGAGAGGCAGCTGTACTGGAAATTGTGGAACGCCTGACCAAGCTCATCAGA; GAGGTCTTTCCAGATACTAAAGTCTATGCTGCTTTGGGAAATCATGATTTTCACCCCAAA; AACCAGTTCCCAGCTGGAAGTAACAACATCTACAATCAGATAGCAGAACTATGGAAACCC; TGGCTTAGTAATGAGTCCATCGCTCTCTTCAAAAAAGGTGCCTTCTACTGTGAGAAGCTG; CCGGGTCCCAGCGGGGCTGGGCGAATTGTGGTCCTCAACACCAATCTGTACTATACCAGC; AATGCGCTGACAGCAGACATGGCGGACCCTGGCCAGCAGTTCCAGTGGCTGGAAGATGTG; CTGACCGATGCATCCAAAGCTGGGGACATGGTGTACATTGTCGGCCACGTGCCCCCGGGG; TTCTTTGAGAAGACGCAAAACAAGGCATGGTTCCGGGAGGGCTTCAATGAAAAATACCTG; AAGGTGGTCCGGAAGCATCATCGCGTCATAGCAGGGCAGTTCTTCGGGCACCACCACACC; GACAGCTTTCGGATGCTCTATGATGATGCAGGTGTCCCCATAAGCGCCATGTTCATCACA; CCTGGAGTCACCCCATGGAAAACCACATTACCTGGAGTGGTCAATGGGGCCAACAATCCA; GCCATCCGGGTGTTCGAATATGACCGAGCCACACTGAGCCTGAAGGACATGGTGACCTAC; TTCATGAACCTGAGCCAGGCGAATGCTCAGGGGACGCCGCGCTGGGAGCTCGAGTACCAG; CTGACCGAGGCCTATGGGGTGCCGGACGCCAGCGCCCACTCCATGCACACAGTGCTGGAC; CGCATCGCTGGCGACCAGAGCACACTGCAGCGCTACTACGTCTATAACTCAGTCAGCTAC; TCTGCTGGGGTCTGCGACGAGGCCTGCAGCATGCAGCACGTGTGTGCCATGCGCCAGGTG; GACATTGACGCTTACACCACCTGTCTGTATGCCTCTGGCACCACGCCCGTGCCCCAGCTC; CCGCTGCTGCTGATGGCCCTGCTGGGCCTGTGCACGCTCGTGCTGTGA; ; Clone variation with respect to NM_014474.2; 63 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014474 unedited; GATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGACGCCTTGGAG; GACTTGGAACACCTGTAACAGGACAAGGAGTCCTGCTCCGGCACGTGGCCACAGAAAACT; ACTTAGGAAGCCTGTGGTGAGAACAACAACAGTGCCTGGAGAATCCCACGGCTCTGGGGA; AGTGAGCCCCGAGGATGAGGCTGCTCGCCTGGCTGATTTTCCTGGCTAACTGGGGAGGTG; CCAGGGCTGAACCAGGTAAGTTCTGGCACATCGCTGACCTGCACCTTGACCCTGACTACA; AGGTATCCAAAGACCCCTTCCAGGTGTGCCCATCAGCTGGATCCCAGCCAGTGCCCGACG; CAGGCCCCTGGGGTGACTACCTCTGTGATTCTCCCTGGGCCCTCATCAACTCCTCCATCT; ATGCCATGAAGGAGATTGAGCCAGAGCCAGACTTCATTCTCTGGACTGGTGATGACACGC; CTCATGTGCCCGATGAGAAACTGGGAGAGGCAGCTGTACTGGAAATTGTGGAACGCCTGA; CCAAGCTCATCAGAGAGGTCTTTCCAGATACTAAAGTCTATGCTGCTTTGGGAAATCATG; ATTTTCACCCCAAAAACCAGTTCCCAGCTGGAAGTAACAACATCTACAATCAGATAGCAG; AACTATGGAAACCCTGGCTTAGTAATGAGTCCATCGCTCTCTTCAAAAAGGTGCCTTCTA; CTGTGAGAAGCTGCCGGGTCCCAGCGGNGCTGGGCGAATTGTGGTCCTCACACCAATCTG; TACTATANCCAGCATGCGCTGACAGCAGACATGGCGGACCCTGGCCAGCAGTTCCAGTGG; CTGGAAGATGTGCTGACCGATGCATCCAAGCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014474 unedited; CGCATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAACGCATGATGAGCTTTGTTTTGT; GAGTAAAACATAATACGCAAAAGAAGTATGCTTGTAGGGGGGTCTTGGGTCGTCATTTCT; TTTCTGTTTCTGGTTTGTTTTTGGAGAGAGTCTGGCTTTGTCGCCCAGGCTGGAGTGCAG; TGGCGCGATCACGGATGGCTGCAGCTCCTGGGGCTTCGCTTCCTGATTCGGTTGTCCTAT; TTCAGTTCACTCCTCAGGCGCGGAGGAAATGGTGGAAGGCCCAGCTCTGGGTGATCCTGG; GCGCTGCCCCCGTTACCCGTTACCAGGAAGAAGGTGAGCCTGGCAGGTCACAGCACGAGC; GTGCCACAGGCCCAGCAGGGCCATCAGCAGCAGCGGGAGCTGGGGCACGGTCGTGGTGCC; AGAGGCATACAGACAGGTGGTGTAAGCGTCAATGTCCACCTGGCGCATGGCACACACGTG; CTGCATGCTGCAGGCCTCGTCGCAGACCCCAGCAGAGTAGCTGACTGAGTTATAGACGTA; GTACCGCTGCAGTGTGCTCTGGTCGCCAGCGATGCGGCCCAGCACTGTGTGCATGGAGTG; GGCGCTGGCGTTCCGCACCCCATTAGCCTCGTCCAGCTGTCCCTCGAGCTCCCAGCGCGG; GCGTCCCCTGAGCATTCCCCTGGCTCAGGCTCATGAAGTAAGTCACCATGCCCTTCAGGC; TCAGTGTGGCTCGGTCATATTCGACACCCGGATGGTTGGATTGTGGCCCCATTGCCCCTC; CAGNTANTGTGGTTTCCTGGGGTGACTCCAGGGGGATGACATGCGCTTATGGGAACCTGC; TTATCTAAACATCCAAAGCTGTGGTGTGTGTGCCCAACACCGCCTTGTTGACCGAGATGC; TCCGACCCTTCAGCTTTTTNATTGACCCTCCGGACCTGCTTGTTTGCGTTTTTAAGAACC; CGTGTCCCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_014474
• Insert Size: 1960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014474.2, NP_055289.2
• RefSeq Size: 1890 bp
• RefSeq ORF: 1368 bp
• Locus ID: 27293
• UniProt ID: Q92485
• Cytogenetics: 1p35.3
• Domains: Metallophos
• Protein Families: Secreted Protein
• Summary: Lipid-modulating phosphodiesterase (PubMed:26095358). Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling (By similarity). Has in vitro phosphodiesterase activity, but the physiological substrate is unknown (PubMed:26095358). Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longest isoform (1).