spindlin 1 (SPIN1) (NM_006717) Human Untagged Clone

SKU SC108548

SPIN1 (untagged)-Human spindlin 1 (SPIN1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: spindlin 1
• Synonyms: SPIN; TDRD24
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108548 sequence for NM_006717 edited (data generated by NextGen Sequencing); ATGAAGACCCCATTCGGAAAGACACCTGGCCAGCGGTCCAGAGCTGATGCAGGCCATGCT; GGAGTATCTGCCAACATGATGAAGAAGAGGACATCCCACAAAAAACATCGGAGCAGTGTG; GGTCCGAGCAAACCTGTTTCCCAGCCCCGGCGGAACATCGTAGGCTGCAGGATTCAGCAT; GGGTGGAAAGAGGGGAATGGCCCTGTTACCCAGTGGAAAGGAACCGTTCTGGACCAGGTG; CCTGTAAATCCTTCTTTGTATCTTATAAAATACGATGGATTTGACTGTGTTTATGGACTA; GAACTTAATAAAGATGAAAGAGTTTCTGCGCTTGAAGTCCTCCCTGATAGAGTTGCGACA; TCTCGAATCAGCGATGCACACTTGGCAGACACAATGATTGGCAAAGCAGTGGAACATATG; TTTGAGACAGAGGATGGTTCTAAAGATGAGTGGAGGGGAATGGTCTTAGCACGTGCACCT; GTCATGAACACATGGTTTTACATTACCTATGAGAAAGACCCTGTCTTGTACATGTACCAA; CTCTTAGATGATTACAAAGAAGGCGACCTTCGCATTATGCCTGATTCCAATGATTCACCT; CCAGCAGAAAGGGAACCAGGAGAAGTTGTGGACAGCCTGGTAGGCAAACAAGTGGAATAT; GCCAAAGAAGATGGCTCGAAAAGGACTGGCATGGTCATTCATCAAGTAGAAGCCAAGCCC; TCCGTCTATTTCATCAAGTTTGATGATGATTTCCATATTTATGTCTACGATTTGGTGAAA; ACATCCTAG; ; Clone variation with respect to NM_006717.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006717 unedited; GAGCCTCGTGCCGAATTCGGCACGAGGATTTTTGCTGAACTCGTAAAAGAGACACTTGGA; TGGTGGATTAACCAGAACACTAACATTCTGTGAAAAGTTTCAAATTGGAGAATATTGAAT; TTTCACCCTAGTCCAGCAGCTCCGCTGCTCACTTAAATACAGATGAATGAAGACCCCATT; CGGAAAGACACCTGGCCAGCGGTCCAGAGCTGATGCAGGCCATGCTGGAGTATCTGCCAA; CATGATGAAGAAGAGGACATCCCACAAAAAACATCGGAGCAGTGTGGGTCCGAGCAAACC; TGTTTCCCAGCCCCGGCGGAACATCGTAGGCTGCAGGATTCAGCATGGGTGGAAAGAGGG; GAATGGCCCTGTTACCCAGTGGAAAGGAACCGTTCTGGACCAGGTGCCTGTAAATCCTTC; TTTGTATCTTATAAAATACGATGGATTTGACTGTGTTTATGGACTAGAACTTAATAAAGA; TGAAAGAGTTTCTGCGCTTGAAGTCCTCCCTGATAGAGTTGCGACATCTCGAATCAGCGA; TGCACACTTGGCAGACACAATGATTGGCAAAGCAGTGGAACATATGTTTGAGACAGANGA; TGGTTCTAAAGATGAGTGGAGGGGAATGGTCTTANCACGTGCACCTGTCATGAACACATG; GTTTTACATTACCTATGANAAAGACCCTGTCTTGTACATGTACCCAACTCTTANATGATT; ACAAAGAAGGCGANCTTCGCATTATGCCTGATTCCATGGATTCACCTNCAGCAGAAAGGG; AACCAGGAAAAGTTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_006717
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006717.1, NP_006708.1
• RefSeq Size: 1506 bp
• RefSeq ORF: 744 bp
• Locus ID: 10927
• UniProt ID: Q9Y657
• Cytogenetics: 9q22.1
• Domains: Spin-Ssty
• Summary: Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway (PubMed:24589551). Overexpression induces metaphase arrest and chromosomal instability. Localizes to active rDNA loci and promotes the expression of rRNA genes (PubMed:21960006). May play a role in cell-cycle regulation during the transition from gamete to embryo. Involved in oocyte meiotic resumption, a process that takes place before ovulation to resume meiosis of oocytes blocked in prophase I: may act by regulating maternal transcripts to control meiotic resumption.[UniProtKB/Swiss-Prot Function]