TGF beta Receptor I (TGFBR1) (NM_004612) Human Untagged Clone

SKU SC108520

TGFBR1 (untagged)-Human transforming growth factor, beta receptor 1 (TGFBR1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TGF beta Receptor I
• Synonyms: AAT5; ACVRLK4; ALK-5; ALK5; ESS1; LDS1; LDS1A; LDS2A; MSSE; SKR4; tbetaR-I; TBR-i; TBRI; TGFR-1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004612 edited; ATGGAGGCGGCGGTCGCTGCTCCGCGTCCCCGGCTGCTCCTCCTCGTGCTGGCGGCGGCG; GCGGCGGCGGCGGCGGCGCTGCTCCCGGGGGCGACGGCGTTACAGTGTTTCTGCCACCTC; TGTACAAAAGACAATTTTACTTGTGTGACAGATGGGCTCTGCTTTGTCTCTGTCACAGAG; ACCACAGACAAAGTTATACACAACAGCATGTGTATAGCTGAAATTGACTTAATTCCTCGA; GATAGGCCGTTTGTATGTGCACCCTCTTCAAAAACTGGGTCTGTGACTACAACATATTGC; TGCAATCAGGACCATTGCAATAAAATAGAACTTCCAACTACTGTAAAGTCATCACCTGGC; CTTGGTCCTGTGGAACTGGCAGCTGTCATTGCTGGACCAGTGTGCTTCGTCTGCATCTCA; CTCATGTTGATGGTCTATATCTGCCACAACCGCACTGTCATTCACCATCGAGTGCCAAAT; GAAGAGGACCCTTCATTAGATCGCCCTTTTATTTCAGAGGGTACTACGTTGAAAGACTTA; ATTTATGATATGACAACGTCAGGTTCTGGCTCAGGTTTACCATTGCTTGTTCAGAGAACA; ATTGCGAGAACTATTGTGTTACAAGAAAGCATTGGCAAAGGTCGATTTGGAGAAGTTTGG; AGAGGAAAGTGGCGGGGAGAAGAAGTTGCTGTTAAGATATTCTCCTCTAGAGAAGAACGT; TCGTGGTTCCGTGAGGCAGAGATTTATCAAACTGTAATGTTACGTCATGAAAACATCCTG; GGATTTATAGCAGCAGACAATAAAGACAATGGTACTTGGACTCAGCTCTGGTTGGTGTCA; GATTATCATGAGCATGGATCCCTTTTTGATTACTTAAACAGATACACAGTTACTGTGGAA; GGAATGATAAAACTTGCTCTGTCCACGGCGAGCGGTCTTGCCCATCTTCACATGGAGATT; GTTGGTACCCAAGGAAAGCCAGCCATTGCTCATAGAGATTTGAAATCAAAGAATATCTTG; GTAAAGAAGAATGGAACTTGCTGTATTGCAGACTTAGGACTGGCAGTAAGACATGATTCA; GCCACAGATACCATTGATATTGCTCCAAACCACAGAGTGGGAACAAAAAGGTACATGGCC; CCTGAAGTTCTCGATGATTCCATAAATATGAAACATTTTGAATCCTTCAAACGTGCTGAC; ATCTATGCAATGGGCTTAGTATTCTGGGAAATTGCTCGACGATGTTCCATTGGTGGAATT; CATGAAGATTACCAACTGCCTTATTATGATCTTGTACCTTCTGACCCATCAGTTGAAGAA; ATGAGAAAAGTTGTTTGTGAACAGAAGTTAAGGCCAAATATCCCAAACAGATGGCAGAGC; TGTGAAGCCTTGAGAGTAATGGCTAAAATTATGAGAGAATGTTGGTATGCCAATGGAGCA; GCTAGGCTTACAGCATTGCGGATTAAGAAAACATTATCGCAACTCAGTCAACAGGAAGGC; ATCAAAATGTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004612 unedited; CAGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGNAATTCGCACCAGGCGAGGTTT; GCTGGGGTGAGGCAGCGGCGCGGCCGGGCCGGGCCGGGCCACAGGCGGTGGCGGCGGGAC; CATGGAGGCGGCGGTCGCTGCTCCGCGTCCCCGGCTGCTCCTCCTCGTGCTGGCGGCGGC; GGCGGCGGCGGCGGCGGCGCTGCTCCCGGGGGCGACGGCGTTACAGTGTTTCTGCCACCT; CTGTACAAAAGACAATTTTACTTGTGTGACAGATGGGCTCTGCTTTGTCTCTGTCACAGA; GACCACAGACAAAGTTATACACAACAGCATGTGTATAGCTGAAATTGACTTAATTCCTCG; AGATAGGCCGTTTGTATGTGCACCCTCTTCAAAAACTGGGTCTGTGACTACAACATATTG; CTGCAATCAGGACCATTGCAATAAAATAGAACTTCCAACTACTGTAAAGTCATCACCTGG; CCTTGGTCCTGTGGAACTGGCAGCTGTCATTGCTGGACCAGTGTGCTTCGTCTGCATCTC; ACTCATGTTGATGGTCTATATCTGCCACAACCGCACTGTCATTCACCATCGAGTGCCAAA; TGAAGAGGACCCTTCATTAGATCGCCCTTTTATTTCAGAGGGTACTACGTTGAAAGACTT; AATTTATGATATGACAACGTCAGGTTCTGGCACAGGTTTACCATTGCTTGTTCAGAGAAC; AATTGCGAGAACTATTGTGTTACAAGAAAGCATTGGCAAAGGTCGATTTGGAGAAGTTTG; GAGAGGAAAGTGGCGGGNGAGAAGAAGTTGCTGTTAAGATATTCTCCTCTAGAGAAGACG; TTCGTGGTTCCGTGAGGCAGAGATTNNATCAAACTGTATGTTACGTCATGAAAACATCCT; GNGATTATAGCAGCAGACATAAAGACATGGTACTGGNACTCGCTCTGGNTGTGGCAGATA; TCAGAGCAGGATACCTTTTGGATACTAACAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004612 unedited; GCCCAATAGGGGTATTGAATTCCATATTACTGCTGCAAAAGAAAGCCTATCCTTCTGTTC; CCTCTCATGAGGTAGAACAATTGACCTCCCAAATTAAAACCCAGGAGCAGATCTGAAGAA; AAAAGGAGAGTTCAGGCAAAGCTGTAGAATTACATTTTGATGCCTTCCTGTTGACTGAGT; TGCGATAATGTTTTCTTAATCCGCAATGCTGTAAGCCTATCTGCTCCATTGGCATACCAA; CATTCTCTCATAATTTTAGCCATTACTCTCAAGGCTTCACAGCTCTGCCATCTGTTTGGG; ATATTTGGCCTTAACTTCTGTTCACAAACAACTTTTCTCATTTCTTCAACTGATGGGTCA; GAAGGTACAAGATCATAATAAGGCAGTTGGTAATCTTCATGAATTCCACCAATGGAACAT; CGTCGAGCAATTTCCCAGAATACTAAGCCCATTGCATAGATGTCAGCACGTTTGAAGGAT; TCAAAATGTTTCATATTTATGGAATCATCGAGAACTTCAGGGGCCATGTACCTTTTTGTT; CCCACTCTGTGGTTTGGAGCAATATCAATGGTATCTGTGGCTGAATCATGTCTTACTGCC; AGTCCTAAGTCTGCAATACAGCAAGTTCCATTCTTCTTTACCAAGATATTCTTTGATTTC; AAATCTCTATGAGCAATGGCTGGCTTTCCTTGGGTACCAACAATCTCCATGTGAAGATGG; GCAAGACCGCTCGCCGTGGACAGAGCAAGTTTTATCATTCCTTCCACAGTAACTGTGTAT; CTGTTTAAGTAATCAAAAGGGATCCATGCTCATGATAATCTGACACCACCAGAGCTGAGT; CCAATACCATTGTCTTTATTGTCTGCTGCTATAAATCCCAAGATGTTTTCATG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004612
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004612.2, NP_004603.1
• RefSeq Size: 6475 bp
• RefSeq ORF: 1512 bp
• Locus ID: 7046
• UniProt ID: P36897
• Cytogenetics: 9q22.33
• Domains: Activin_recp, GS, pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Chronic myeloid leukemia, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, MAPK signaling pathway, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway
• Summary: The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]; Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 5' coding region, compared to variant 3. The encoded isoform (1) is shorter than isoform 3.