Aldolase (ALDOA) (NM_000034) Human Untagged Clone

CAT#: SC108418

ALDOA (untagged)-Human aldolase A, fructose-bisphosphate (ALDOA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Aldolase
• Synonyms: ALDA; GSD12; HEL-S-87p
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108418 sequence for NM_000034 edited (data generated by NextGen Sequencing)
  ATGCCCTACCAATATCCAGCACTGACCCCGGAGCAGAAGAAGGAGCTGTCTGACATCGCT
  CACCGCATCGTGGCACCTGGCAAGGGCATCCTGGCTGCAGATGAGTCCACTGGGAGCATT
  GCCAAGCGGCTGCAGTCCATTGGCACCGAGAACACCGAGGAGAACCGGCGCTTCTACCGC
  CAGCTGCTGCTGACAGCTGACGACCGCGTGAACCCCTGCATTGGGGGTGTCATCCTCTTC
  CATGAGACACTCTACCAGAAGGCGGATGATGGGCGTCCCTTCCCCCAAGTTATCAAATCC
  AAGGGCGGTGTTGTGGGCATCAAGGTAGACAAGGGCGTGGTCCCCCTGGCAGGGACAAAT
  GGCGAGACTACCACCCAAGGGTTGGATGGGCTGTCTGAGCGCTGTGCCCAGTACAAGAAG
  GACGGAGCTGACTTCGCCAAGTGGCGTTGTGTGCTGAAGATTGGGGAACACACCCCCTCA
  GCCCTCGCCATCATGGAAAATGCCAATGTTCTGGCCCGTTATGCCAGTATCTGCCAGCAG
  AATGGCATTGTGCCCATCGTGGAGCCTGAGATCCTCCCTGATGGGGACCATGACTTGAAG
  CGCTGCCAGTATGTGACCGAGAAGGTGCTGGCTGCTGTCTACAAGGCTCTGAGTGACCAC
  CACATCTACCTGGAAGGCACCTTGCTGAAGCCCAACATGGTCACCCCAGGCCATGCTTGC
  ACTCAGAAGTTTTCTCATGAGGAGATTGCCATGGCGACCGTCACAGCGCTGCGCCGCACA
  GTGCCCCCCGCTGTCACTGGGATCACCTTCCTGTCTGGAGGCCAGAGTGAGGAGGAGGCG
  TCCATCAACCTCAATGCCATTAACAAGTGCCCCCTGCTGAAGCCCTGGGCCCTGACCTTC
  TCCTACGGCCGAGCCCTGCAGGCCTCTGCCCTGAAGGCCTGGGGCGGGAAGAAGGAGAAC
  CTGAAGGCTGCGCAGGAGGAGTATGTCAAGCGAGCCCTGGCCAACAGCCTTGCCTGTCAA
  GGAAAGTACACTCCGAGCGGTCAGGCTGGGGCTGCTGCCAGCGAGTCCCTCTTCGTCTCT
  AACCACGCCTATTAA
  
  Clone variation with respect to NM_000034.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000034 unedited
  NTTGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGATTTATT
  TCTCTTGCAACCAAGGCCTCCGTCTGGATTTCCAAGGAAGAATTTCCTCTGAAGCACCGG
  AACTTGCTACTACCAGCACCATGCCCTACCAATATCCAGCACTGACCCCGGAGCAGAAGA
  AGGAGCTGTCTGACATCGCTCACCGCATCGTGGCACCTGGCAAGGGCATCCTGGCTGCAG
  ATGAGTCCACTGGGAGCATTGCCAAGCGGCTGCAGTCCATTGGCACCGAGAACACCGAGG
  AGAACCGGCGCTTCTACCGCCAGCTGCTGCTGACAGCTGACGACCGCGTGAACCCCTGCA
  TTGGGGGTGTCATCCTCTTCCATGAGACACTCTACCAGAAGGCGGATGATGGGCGTCCCT
  TCCCCCAAGTTATCAAATCCAAGGGCGGTGTTGTGGGCATCAAGGTAGACAAGGGCGTGG
  TCCCCCTGGCAGGGACAAATGGCGAGACTACCACCCAAGGGTTGGATGGGCTGTCTGAGC
  GCTGTGCCCAGTACAAGAAGGACGGAGCTGACTTCGCCAAGTGGCGTTGTGTGCTGAAGA
  TTGGGGAACACACCCCCTCAGCCCTCGCCATCATGGAAAATGCCAATGTTCTGGCCCGTT
  ATGCCAGTATCTGCCAGCAGAATGGCATTGTGCCCATCGTGGAGCCTGAGATCCTCCCTG
  ATGGGGACCATGACTTGAAGCGCTGCCAGTATGTGACCGAGAAGGTGCTGGCTGCTGTCT
  ACAAGGCTCTGAGTGACCACCACATCTACCTGGAAGGCACCTTGCTGAAGCCCACATGGG
  TCACCCAGCCATGCTGCCTTTCANAAGTTTCTCATGAGAGATGCCATGGCGACCGTCAAG
  CGCTGCGCCGACAGTGCCCCGCTGTACTGGGACACCTTCTGTCTGAGCAGAGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000034 unedited
  TTTGAATACTGTTTATTTGGCTGTGTGCCGGAAAGGTGATGGACTTAGCATTCACATACG
  ACACCACACACCACTGTCACGAGGGAAGAAAGAGCGCGGGCAAGCCAGCCTGGAGCCCCG
  AGGAGGCGGCCTCCTCTTCAAGAGTGGGAGGGGGCAGGGCCTGGAGTGTTGGGGGCAGCC
  TGGGAACACCTCCGCTTAATAGGCGTGGTTAGAGACGAAGAGGGACTCGCTGGCAGCAGC
  CCCAGCCTGACCGCTCGGAGTGTACTTTCCTTGACAGGCAAGGCTGTTGGCCAGGGCTTC
  GCTTGACATACTCCTCCTGCGCAGCCTTCAGGTTCTCCTTCTTCCCGCCCCAGGCCTTCA
  GGGCAGAGGCCTGCAGGGCTCGGCCGTAGGAGAAGGTCAGGGCCCAGGGCTTCAGCAGGG
  GGCACTTGTTAATGGCATTGAGGTTGATGGACGCCTCCTCCTCACTCTGGCCTCCAGACA
  GGAAGGTGATCCCAGTGACAGCGGGGGGCACTGTGCGGCGCATCGCTGTGACGGTCGCCA
  TGGCAATCTCCTCATGAGAAAACTTCTGAGTGCAAGCATGGCCTGGGGTGACCATGTTGG
  GCTTCAGCAAGGTGCCTTCCAGGTAGATGTGGTGGTCACTCAGAGCCTTGTAGACAGCAG
  CCAGCACCTTCTCGGTCACATACTGGCAGCGCTTCAAGTCATGGTCCCCATCAGGGAGGA
  TCTCATGGCTCACGATGGGCACAATGCCATTCTGCTGGCAGATACTGGCATAACGGGCCA
  GAACATTGGCATTTTCCATGATGGCGATGGCTGAGGGGGTGTGTTCCCCCAATCTCAGCA
  CACAACGCCACTTGGCGAAAGTCAGCTCGTCCTTTCTGTACTGGGCACAGCGCTCAGACA
  GNCCATCCNACCCTTGGGTGGTAGTCTCGCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000034
• Insert Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000034.2, NP_000025.1
• RefSeq Size: 2353 bp
• RefSeq ORF: 1095 bp
• Locus ID: 226
• UniProt ID: P04075
• Cytogenetics: 16p11.2
• Domains: glycolytic_enzy
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway
• Gene Summary: This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Sep 2017]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 2, 3, 4, 7, 8, 9, and 10 encode the same isoform.