Aldolase (ALDOA) (NM_000034) Human Untagged Clone

SKU SC108418

ALDOA (untagged)-Human aldolase A, fructose-bisphosphate (ALDOA), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Aldolase
• Synonyms: ALDA; GSD12; HEL-S-87p
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108418 sequence for NM_000034 edited (data generated by NextGen Sequencing); ATGCCCTACCAATATCCAGCACTGACCCCGGAGCAGAAGAAGGAGCTGTCTGACATCGCT; CACCGCATCGTGGCACCTGGCAAGGGCATCCTGGCTGCAGATGAGTCCACTGGGAGCATT; GCCAAGCGGCTGCAGTCCATTGGCACCGAGAACACCGAGGAGAACCGGCGCTTCTACCGC; CAGCTGCTGCTGACAGCTGACGACCGCGTGAACCCCTGCATTGGGGGTGTCATCCTCTTC; CATGAGACACTCTACCAGAAGGCGGATGATGGGCGTCCCTTCCCCCAAGTTATCAAATCC; AAGGGCGGTGTTGTGGGCATCAAGGTAGACAAGGGCGTGGTCCCCCTGGCAGGGACAAAT; GGCGAGACTACCACCCAAGGGTTGGATGGGCTGTCTGAGCGCTGTGCCCAGTACAAGAAG; GACGGAGCTGACTTCGCCAAGTGGCGTTGTGTGCTGAAGATTGGGGAACACACCCCCTCA; GCCCTCGCCATCATGGAAAATGCCAATGTTCTGGCCCGTTATGCCAGTATCTGCCAGCAG; AATGGCATTGTGCCCATCGTGGAGCCTGAGATCCTCCCTGATGGGGACCATGACTTGAAG; CGCTGCCAGTATGTGACCGAGAAGGTGCTGGCTGCTGTCTACAAGGCTCTGAGTGACCAC; CACATCTACCTGGAAGGCACCTTGCTGAAGCCCAACATGGTCACCCCAGGCCATGCTTGC; ACTCAGAAGTTTTCTCATGAGGAGATTGCCATGGCGACCGTCACAGCGCTGCGCCGCACA; GTGCCCCCCGCTGTCACTGGGATCACCTTCCTGTCTGGAGGCCAGAGTGAGGAGGAGGCG; TCCATCAACCTCAATGCCATTAACAAGTGCCCCCTGCTGAAGCCCTGGGCCCTGACCTTC; TCCTACGGCCGAGCCCTGCAGGCCTCTGCCCTGAAGGCCTGGGGCGGGAAGAAGGAGAAC; CTGAAGGCTGCGCAGGAGGAGTATGTCAAGCGAGCCCTGGCCAACAGCCTTGCCTGTCAA; GGAAAGTACACTCCGAGCGGTCAGGCTGGGGCTGCTGCCAGCGAGTCCCTCTTCGTCTCT; AACCACGCCTATTAA; ; Clone variation with respect to NM_000034.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000034 unedited; NTTGTCAGAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGATTTATT; TCTCTTGCAACCAAGGCCTCCGTCTGGATTTCCAAGGAAGAATTTCCTCTGAAGCACCGG; AACTTGCTACTACCAGCACCATGCCCTACCAATATCCAGCACTGACCCCGGAGCAGAAGA; AGGAGCTGTCTGACATCGCTCACCGCATCGTGGCACCTGGCAAGGGCATCCTGGCTGCAG; ATGAGTCCACTGGGAGCATTGCCAAGCGGCTGCAGTCCATTGGCACCGAGAACACCGAGG; AGAACCGGCGCTTCTACCGCCAGCTGCTGCTGACAGCTGACGACCGCGTGAACCCCTGCA; TTGGGGGTGTCATCCTCTTCCATGAGACACTCTACCAGAAGGCGGATGATGGGCGTCCCT; TCCCCCAAGTTATCAAATCCAAGGGCGGTGTTGTGGGCATCAAGGTAGACAAGGGCGTGG; TCCCCCTGGCAGGGACAAATGGCGAGACTACCACCCAAGGGTTGGATGGGCTGTCTGAGC; GCTGTGCCCAGTACAAGAAGGACGGAGCTGACTTCGCCAAGTGGCGTTGTGTGCTGAAGA; TTGGGGAACACACCCCCTCAGCCCTCGCCATCATGGAAAATGCCAATGTTCTGGCCCGTT; ATGCCAGTATCTGCCAGCAGAATGGCATTGTGCCCATCGTGGAGCCTGAGATCCTCCCTG; ATGGGGACCATGACTTGAAGCGCTGCCAGTATGTGACCGAGAAGGTGCTGGCTGCTGTCT; ACAAGGCTCTGAGTGACCACCACATCTACCTGGAAGGCACCTTGCTGAAGCCCACATGGG; TCACCCAGCCATGCTGCCTTTCANAAGTTTCTCATGAGAGATGCCATGGCGACCGTCAAG; CGCTGCGCCGACAGTGCCCCGCTGTACTGGGACACCTTCTGTCTGAGCAGAGGN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000034 unedited; TTTGAATACTGTTTATTTGGCTGTGTGCCGGAAAGGTGATGGACTTAGCATTCACATACG; ACACCACACACCACTGTCACGAGGGAAGAAAGAGCGCGGGCAAGCCAGCCTGGAGCCCCG; AGGAGGCGGCCTCCTCTTCAAGAGTGGGAGGGGGCAGGGCCTGGAGTGTTGGGGGCAGCC; TGGGAACACCTCCGCTTAATAGGCGTGGTTAGAGACGAAGAGGGACTCGCTGGCAGCAGC; CCCAGCCTGACCGCTCGGAGTGTACTTTCCTTGACAGGCAAGGCTGTTGGCCAGGGCTTC; GCTTGACATACTCCTCCTGCGCAGCCTTCAGGTTCTCCTTCTTCCCGCCCCAGGCCTTCA; GGGCAGAGGCCTGCAGGGCTCGGCCGTAGGAGAAGGTCAGGGCCCAGGGCTTCAGCAGGG; GGCACTTGTTAATGGCATTGAGGTTGATGGACGCCTCCTCCTCACTCTGGCCTCCAGACA; GGAAGGTGATCCCAGTGACAGCGGGGGGCACTGTGCGGCGCATCGCTGTGACGGTCGCCA; TGGCAATCTCCTCATGAGAAAACTTCTGAGTGCAAGCATGGCCTGGGGTGACCATGTTGG; GCTTCAGCAAGGTGCCTTCCAGGTAGATGTGGTGGTCACTCAGAGCCTTGTAGACAGCAG; CCAGCACCTTCTCGGTCACATACTGGCAGCGCTTCAAGTCATGGTCCCCATCAGGGAGGA; TCTCATGGCTCACGATGGGCACAATGCCATTCTGCTGGCAGATACTGGCATAACGGGCCA; GAACATTGGCATTTTCCATGATGGCGATGGCTGAGGGGGTGTGTTCCCCCAATCTCAGCA; CACAACGCCACTTGGCGAAAGTCAGCTCGTCCTTTCTGTACTGGGCACAGCGCTCAGACA; GNCCATCCNACCCTTGGGTGGTAGTCTCGCCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_000034
• Insert Size: 1530 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000034.2, NP_000025.1
• RefSeq Size: 2353 bp
• RefSeq ORF: 1095 bp
• Locus ID: 226
• UniProt ID: P04075
• Cytogenetics: 16p11.2
• Domains: glycolytic_enzy
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway
• Summary: This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Sep 2017]; Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 2, 3, 4, 7, 8, 9, and 10 encode the same isoform.