DHFRL1 (DHFR2) (NM_176815) Human Untagged Clone

SKU SC108348

DHFRL1 (untagged)-Human dihydrofolate reductase-like 1 (DHFRL1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: DHFRL1
• Synonyms: DHFRL1; DHFRP4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108348 sequence for NM_176815 edited (data generated by NextGen Sequencing); ATGTTTCTTTTGCTAAACTGCATCGTCGCTGTGTCCCAAAACATGGGCATCGGCAAGAAC; GGGGACCTGCCCAGGCCGCCGCTCAGGAATGAATTCAGGTATTTCCAGAGAATGACCACA; ACTTCTTCAGTAGAGGGTAAACAGAATCTGGTGATTATGGGTAGGAAGACCTGGTTCTCC; ATTCCTGAGAAGAATCGACCTTTAAAGGATAGAATTAATTTAGTTCTCAGCAGAGAACTC; AAGGAACCTCCACAAGGAGCTCATTTTCTTGCCAGAAGTTTGGATGATGCCTTAAAACTT; ACTGAACGACCAGAATTAGCAAATAAAGTAGACATGATTTGGATAGTTGGTGGCAGTTCT; GTTTATAAGGAAGCCATGAATCACCTAGGCCATCTTAAACTATTTGTGACAAGGATCATG; CAGGACTTTGAAAGTGACACGTTTTTTTCAGAAATTGACTTGGAGAAATATAAACTTCTG; CCTGAATACCCAGGTGTTCTCTCTGATGTCCAGGAGGGGAAACACATCAAGTACAAATTT; GAAGTATGTGAGAAGGATGATTAA; ; Clone variation with respect to NM_176815.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_176815 unedited; NGGGGGTTCAGCAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCACA; CATCTGCGCGCCTGTGCGGCCCCTGCTTCTGCGGATGCTGAGGCACGTAAAAAAATTTGA; AGAAGGGAATTTCGCGGCATTCTTGGCCTGGCTTCCTGGCGTAGCCAGCAAGTTCGGAGG; TGTTAACCGCTGCTGTCATGTTTCTTTTGCTAAACTGCATCGTCGCTGTGTCCCAAAACA; TGGGCATCGGCAAGAACGGGGACCTGCCCAGGCCGCCGCTCAGGAATGAATTCAGGTATT; TCCAGAGAATGACCACAACTTCTTCAGTAGAGGGTAAACAGAATCTGGTGATTATGGGTA; GGAAGACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGATAGAATTAATTTAG; TTCTCAGCAGAGAACTCAAGGAACCTCCACAAGGAGCTCATTTTCTTGCCAGAAGTTTGG; ATGATGCCTTAAAACTTACTGAACGACCAGAATTAGCAAATAAAGTAGACATGATTTGGA; TAGTTGGTGGCAGTTCTGTTTATAAGGAAGCCATGAATCACCTAGGCCATCTTAAACTAT; TTGTGACAAGGATCATGCAGGACTTTGAAAGTGACACGTTTTTTTCAGAAATTGACTTGG; AGAAATATAAACTTCTGCCTGAATACCCAGGTGTTCTCTCTGATGTCCAGGAGGGGAAAC; ACATCAAGTACAAATTTGAAGTATGTGAGAAGGATGATTAATATGAAANGGTGTTTCTGG; TTTAAGNTGTTCCCCCTCCCTCTGAGAAAAGTATGCATTTTTACATTAGAAAAAGGACTT; TTGTTGACTTCAGATCTATGGNATAATTATTTCTAAGCACGTGTTTTTATTCCTCACTAA; TCTGGCCTTNTCCAGATCCAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_176815 unedited; NNGGGTCCTGNCATGNACCGCGGCACGCAATCTAAGATCGAGTTTTTTTTTTTTTTTTTT; ACTTTTGCTTTATCTTCAATGCACTTCTTTCCAAATGCCCCAAATCTGGCAATAACCTAT; TATTTTTAAGAGAAGGGATCTCACTATGGTGGCCAGGCTGCTCTGAACTCTTGGGCTCAA; GCCATCCTTTTGCTTAGCCTCCTGAGTAGCTGGGACTACAAGCATGGGCCATCCCACCCA; GCTAATCTATTAGTATTTTTACTGTTTCTTTTTAAAGCTAGCTCCATAGCTGGAATATTG; CCTGTGTTGGATACTATTTGCTGGATCCACGTCATTCTCTGGTTTGTGTACGCCTTCATT; TTGCTGGAGTATTTTCTCCGATAGCTTCCCAAGAAAGGGTACATGTAACCCCTGAGTCTT; TGCTTGTCTAAAAATGTATTATTTTACCTTCACCCTTGATTCTTTGCCTGAATATAGATT; TATCAGTTGAAAATAACTTTATAGAATTCTGAAGGCATGGTTCCGTTGTTTTCAGATTCT; TTTTTGAGACAGGGTCTCTTCTGTCACCCAGGCAGGAGTGCAGTGGCAAAATCACGACTC; ACTGCAGCCTTGAACTCATGGGCTCAATTGAATCCTCCATCTCAGCCCCGTGAGTAGACG; AGACTACAGGTGCGTGCCACAATGCCCAGCCAATTTTTGTATCTTCTGTAGAGACAGGGC; TCCACCATGTTGCCCAAGCTGGTCTCAAGTGATCCACCCACCTCATCCCCCCAAATGCTG; GGACCCACAGCGTGAGCCACCCCTGCTGGCCAGTTTTAAATTCCCTCCCTAACTCTTTCA; TTTCTCAAAGGGCTCTCAACCTCGTGGGGCCCAATAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_176815
• Insert Size: 3300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_176815.2, NP_789785.1
• RefSeq Size: 3772 bp
• RefSeq ORF: 564 bp
• Locus ID: 200895
• UniProt ID: Q86XF0
• Cytogenetics: 3q11.2
• Protein Families: Druggable Genome
• Summary: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) differs in its 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.