SMC6L1 (SMC6) (NM_024624) Human Untagged Clone

SKU SC108334

SMC6 (untagged)-Human structural maintenance of chromosomes 6 (SMC6), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: SMC6L1
• Synonyms: hSMC6; SMC-6; SMC6L1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024624, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCAAAAGAAAGGAAGAAAATTTTTCCTCTCCTAAAAATGCCAAAAGGCCAAGACAAGAAGAATTGG; AGGATTTTGATAAAGATGGTGACGAAGACGAATGTAAAGGTACTACTTTGACTGCAGCAGAAGTTGGAAT; AATTGAGAGTATTCACCTAAAAAACTTCATGTGTCATTCAATGCTTGGACCTTTTAAGTTTGGTTCTAAT; GTCAACTTTGTTGTTGGCAACAATGGAAGTGGGAAGAGTGCAGTACTCACAGCTCTCATAGTCGGTCTTG; GTGGAAGAGCAGTTGCTACTAATAGAGGATCCTCTTTAAAAGGTTTTGTGAAAGATGGACAGAACTCTGC; AGATATCTCAATAACATTGAGGAACAGAGGAGATGATGCCTTTAAAGCCAGTGTGTATGGTAACTCTATA; CTTATACAGCAACACATCAGCATAGATGGAAGTCGATCTTATAAACTTAAAAGTGCAACAGGCTCCGTGG; TTTCCACGAGGAAAGAAGAGCTGATTGCAATTCTTGATCATTTTAACATCCAGGTGGATAATCCAGTTTC; TGTTTTAACACAAGAAATGAGCAAGCAGTTCTTACAGTCTAAAAATGAAGGAGACAAATACAAATTCTTC; ATGAAAGCAACGCAACTTGAACAGATGAAGGAAGATTATTCATACATTATGGAAACGAAAGAAAGAACAA; AGGAGCAGATACATCAAGGAGAAGAGCGGCTTACTGAACTAAAGCGCCAGTGTGTAGAGAAAGAGGAACG; TTTTCAAAGTATTGCTGGTTTAAGTACAATGAAGACTAATTTAGAGTCCTTGAAACATGAAATGGCTTGG; GCAGTGGTCAATGAAATTGAAAAACAATTGAATGCCATCAGAGATAATATCAAAATTGGAGAAGATCGTG; CTGCTAGACTTGACAGGAAAATGGAAGAACAGCAGGTCAGACTTAATGAGGCAGAACAAAAGTACAAGGA; TATTCAAGACAAACTAGAAAAGATTAGTGAAGAGACAAATGCACGAGCACCAGAATGTATGGCATTGAAA; GCAGATGTTGTTGCTAAGAAAAGGGCCTATAATGAAGCTGAGGTTTTATATAACCGATCCTTAAACGAAT; ATAAAGCATTAAAGAAAGATGATGAGCAGCTTTGTAAACGAATTGAAGAGCTGAAAAAAAGTACTGACCA; ATCTTTGGAACCTGAACGGTTGGAAAGACAAAAAAAAATATCTTGGTTAAAAGAGAGAGTAAAGGCCTTT; CAAAATCAAGAAAATTCAGTCAATCAAGAGATCGAACAGTTTCAGCAAGCCATAGAAAAGGACAAAGAAG; AACATGGCAAAATTAAGAGAGAAGAATTAGATGTGAAGCATGCACTGAGCTACAATCAGAGGCAACTGAA; AGAATTGAAAGATAGTAAAACTGATCGACTCAAAAGATTTGGCCCTAATGTTCCAGCTCTTCTTGAAGCC; ATAGATGATGCTTATAGACAAGGACATTTTACCTATAAACCTGTAGGCCCTTTAGGAGCTTGCATTCATC; TTCGGGACCCAGAACTTGCTTTGGCTATTGAATCTTGCTTAAAAGGGCTTCTGCAGGCCTATTGTTGCCA; TAATCATGCTGATGAAAGGGTCCTTCAGGCACTCATGAAAAGGTTTTATTTACCAGGGACCTCACGGCCA; CCGATAATAGTTTCTGAGTTTCGGAATGAGATATATGATGTAAGACACAGAGCTGCTTATCATCCAGACT; TTCCAACAGTTCTGACAGCTTTAGAAATAGATAATGCGGTTGTGGCAAATAGCCTAATTGACATGAGAGG; CATAGAGACAGTGCTACTAATCAAAAATAATTCTGTAGCTCGTGCAGTAATGCAGTCCCAAAAGCCACCC; AAAAATTGTAGAGAAGCTTTTACTGCTGATGGTGATCAAGTTTTTGCAGGACGTTATTATTCATCTGAAA; ATACAAGACCTAAGTTCCTAAGCAGAGATGTGGATTCTGAAATAAGTGACTTGGAGAATGAGGTTGAAAA; TAAGACGGCCCAGATATTAAATCTTCAGCAACATTTATCTGCCCTTGAAAAAGATATTAAACACAATGAG; GAACTTCTTAAAAGGTGCCAACTACATTATAAAGAACTAAAGATGAAAATAAGAAAAAATATTTCTGAAA; TTCGGGAACTTGAGAACATAGAAGAACACCAGTCTGTAGATATTGCAACTTTGGAAGATGAAGCTCAGGA; AAATAAAAGCAAAATGAAAATGGTTGAGGAACATATGGAGCAACAAAAAGAAAATATGGAGCATCTTAAA; AGTCTGAAAATAGAAGCAGAAAATAAGTATGATGCAATTAAATTCAAAATTAATCAACTATCGGAGCTAG; CAGACCCACTTAAGGATGAATTAAACCTTGCTGATTCTGAAGTGGATAACCAAAAACGAGGGAAACGACA; TTATGAAGAAAAACAAAAAGAACACTTGGATACCTTAAATAAAAAGAAACGAGAACTGGATATGAAAGAG; AAAGAACTAGAGGAGAAAATGTCACAAGCAAGACAAATCTGCCCAGAGCGTATAGAAGTAGAAAAATCTG; CATCAATTCTGGACAAAGAAATTAATCGATTAAGGCAGAAGATACAGGCAGAACATGCTAGTCATGGAGA; TCGAGAGGAAATAATGAGGCAGTACCAAGAAGCAAGAGAGACCTATCTTGATCTGGATAGTAAAGTGAGG; ACTTTAAAAAAGTTTATTAAATTACTGGGAGAAATCATGGAGCACAGATTCAAGACATATCAACAATTTA; GAAGGTGTTTGACTTTACGATGCAAATTATACTTTGACAACTTACTATCTCAGCGGGCCTATTGTGGAAA; AATGAATTTTGACCACAAGAATGAAACTCTAAGTATATCAGTTCAGCCTGGAGAAGGAAATAAAGCTGCT; TTCAATGACATGAGAGCCTTGTCTGGAGGTGAACGTTCTTTCTCCACAGTGTGTTTTATTCTTTCCCTGT; GGTCCATCGCAGAATCTCCTTTCAGATGCCTGGATGAATTTGATGTCTACATGGATATGGTTAATAGGAG; AATTGCCATGGACTTGATACTGAAGATGGCAGATTCCCAGCGTTTTAGACAGTTTATCTTGCTCACACCT; CAAAGCATGAGTTCACTTCCATCCAGTAAACTGATAAGAATTCTCCGAATGTCTGATCCTGAAAGAGGAC; AAACTACATTGCCTTTCAGACCTGTGACTCAAGAAGAAGATGATGACCAAAGGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024624 unedited; TAGAATTTTGGTAATACGACTCACTATAGGGCGGCCGCGATTCGGCACCAGCTGCGGCCG; GCACGCGGTTCCCAGGGGCCAGCGGCGGTCAGCCGAGGTCGAGACGCCCGCAGGGTGGCC; TTAGCGGCCGGTCGTACCACGGCAGCCCCGCCGATCAGGTTCCTTTGGGAGACTTCGACT; TGTTGGCGACCTGATGGCCAAAAGAAAGGAAGAAAATTTTTCCTCTCCTAAAAATGCCAA; AAGGCCAAGACAAGAAGAATTGGAGGATTTTGATAAAGATGGTGACGAAGACGAATGTAA; AGGTACTACTTTGACTGCAGCAGAAGTTGGAATAATTGAGAGTATTCACCTAAAAAACTT; CATGTGTCATTCAATGCTTGGACCTTTTAAGTTTGGTTCTAATGTCAACTTTGTTGTTGG; CAACAATGGAAGTGGGAAGAGTGCAGTACTCACAGCTCTCATAGTCGGTCTTGGTGGAAG; AGCAGTTGCTACTAATAGAGGATCCTCTTTAAAAGGTTTTGTGAAAGATGGACAGAACTC; TGCAGATATCTCAATAACATTGAGGAACAGAGGAGATGATGCCTTTAAAGCCAGTGTGTA; TGGTAACTCTATACTTATACAGCAACACATCAGCATAGATGGAAGTCGATCTTATAAACT; TAAAAGTGCAACAGGCTCCGTGGTTTCCACGAGGAAAGAAGAGCTGNATTGCATTCTTGA; TCATTTTAACATCCAGGTGGATAATCCAGTTTCTGTTTTAACACANAGAAATGAGCAGCA; GTTCTTACAGTCTANAAATGAAGGGAGACAATACANATTCTTCATGAANAGCACGCAACT; TGNACAGATGAAGGNNAGATATTCATACATTATGGNNAACGAAGAAGACCAAGAGCAGAT; CCTCAAGGGAAGAGCGGCTACTGACTAAGCGCATGTGTANAGAAAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024624 unedited; CCGCGGCCGCAATTTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATACTCTACTTCATTTT; TAATTTGGAAAAGCACATGTTAAAAATAATTTTAATACAAAGGCATTATAAGTGTAAATT; TAAAAATCAGACTTTGTATGATCAGTGGGAAATGTTTAAAATATTCAGCACACACTTTCC; TCCTCCTGGAAATTCTGATTAGAAAGCCCAAGTTTTTGAACTTCTCATTTGGCAAACCTC; AATTTAAGACTAAAGTATCTTAAACTTCTTCCTCCACCAAAAACACAAACAAAGTCCAGT; TTTCCTCAATAACTAGAGAACAGTCCCTGCTCTTAATATACTGCCCTTTTTCTGAAAATA; GTTCAATTTCATATTTATTCTAATATTTATTGCTACTAATGTAAGGAATGCTTCTTGATA; TCCAATACATTCTTAAAAAGTTTAGCATGAATCCTAGTATCAAACAAATAATACAGGGAG; CAATAAAATTCATGTTTTCTAAAATAAAAAGTTCATAAATATCATTTTTTACAATAACTA; TATTTGGCCATTCTAAGCAGCACACTTTTTAACACCCCTCATTTAGGATGAGTCTTAGAA; TTGATGTCAGCTCACCATCAACTGCCTGGCCTCAGGCTCCTGGAGCCATGACCTACTCTC; CTGCTACATGAGGATCCCCCCCTCCCTCCCAGTTTTCCCAACCCTTCTACCAACCTGATA; TCCCCCAGCCCTCTAGCCTGTGACTCCTCACTGACGACAATCCTGAACAGCCCTTGGCCT; GCACAACTGAGGCCTTTCCAATCCGCCACCCTTGATGGAGTCAAAGGGAAAAACTGGGTC; TTATTCCGTCCCGAACCTGGTTTTACCTCTTTGCTGGGGGACCGTCAAAGGCGGCCCAAC; AGCCTGGGACTAATTGGTCAANCTTCGTGTCCCTCCACGGGCCGTATTCCCTGCGCCACC; ACCTTGGAATGACAACCCCCTCCCTCAACCTCTCGCGTACTTTACAGCCGATCTTGGAAC; CAAAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_024624
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024624.3, NP_078900.1
• RefSeq Size: 5151 bp
• RefSeq ORF: 3276 bp
• Locus ID: 79677
• UniProt ID: Q96SB8
• Cytogenetics: 2p24.2
• Domains: SMC_C
• Summary: Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed:26983541).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein.