GJA5 (NM_005266) Human Untagged Clone

SKU SC108214

GJA5 (untagged)-Human gap junction protein, alpha 5, 40kDa (GJA5), transcript variant A

Product Data

• Type: Human Untagged Clone
• Target Symbol: GJA5
• Synonyms: ATFB11; CX40
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005266, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGGCGATTGGAGCTTCCTGGGAAATTTCCTGGAGGAAGTACACAAGCACTCGACCGTGGTAGGCAAGG; TCTGGCTCACTGTCCTCTTCATATTCCGTATGCTCGTGCTGGGCACAGCTGCTGAGTCTTCCTGGGGGGA; TGAGCAGGCTGATTTCCGGTGTGATACGATTCAGCCTGGCTGCCAGAATGTCTGCTACGACCAGGCTTTC; CCCATCTCCCACATTCGCTACTGGGTGCTGCAGATCATCTTCGTCTCCACGCCCTCTCTGGTGTACATGG; GCCACGCCATGCACACTGTGCGCATGCAGGAGAAGCGCAAGCTACGGGAGGCCGAGAGGGCCAAAGAGGT; CCGGGGCTCTGGCTCTTACGAGTACCCGGTGGCAGAGAAGGCAGAACTGTCCTGCTGGGAGGAAGGGAAT; GGAAGGATTGCCCTCCAGGGCACTCTGCTCAACACCTATGTGTGCAGCATCCTGATCCGCACCACCATGG; AGGTGGGCTTCATTGTGGGCCAGTACTTCATCTACGGAATCTTCCTGACCACCCTGCATGTCTGCCGCAG; GAGTCCCTGTCCCCACCCGGTCAACTGTTACGTATCCCGGCCCACAGAGAAGAATGTCTTCATTGTCTTT; ATGCTGGCTGTGGCTGCACTGTCCCTCCTCCTTAGCCTGGCTGAACTCTACCACCTGGGCTGGAAGAAGA; TCAGACAGCGATTTGTCAAACCGCGGCAGCACATGGCTAAGTGCCAGCTTTCTGGCCCCTCTGTGGGCAT; AGTCCAGAGCTGCACACCACCCCCCGACTTTAATCAGTGCCTGGAGAATGGCCCTGGGGGAAAATTCTTC; AATCCCTTCAGCAATAATATGGCCTCCCAACAAAACACAGACAACCTGGTCACCGAGCAAGTACGAGGTC; AGGAGCAGACTCCTGGGGAAGGTTTCATCCAGGTTCGTTATGGCCAGAAGCCTGAGGTGCCCAATGGAGT; CTCACCAGGTCACCGCCTTCCCCATGGCTATCATAGTGACAAGCGACGTCTTAGTAAGGCCAGCAGCAAG; GCAAGGTCAGATGACCTATCAGTGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005266 unedited; GTCACATTTTGTATACGACTCACTTAGGCGGCCGCGATTCGCCGAGGAACAACTGACAGG; CTCAGAGCAAAAGCGTGGGCAGTTGGAGAAGAAGCAGCCAGAGTGTGAAGAAGCCCACGG; AAGGAAAGTCCAGGGAGGAGGAAAAGAAGCAGAAGTTTTGGCATCTGTTCCCTGGCTGTG; CCAAGATGGGCGATTGGAGCTTCCTGGGAAATTTCCTGGAGGAAGTACACAAGCACTCGA; CCGTGGTAGGCAAGGTCTGGCTCACTGTCCTCTTCATATTCCGTATGCTCGTGCTGGGCA; CAGCTGCTGAGTCTTCCTGGGGGGATGAGCAGGCTGATTTCCGGTGTGATACGATTCAGC; CTGGCTGCCAGAATGTCTGCTACGACCAGGCTTTCCCCATCTCCCACATTCGCTACTGGG; TGCTGCAGATCATCTTCGTCTCCACGCCCTCTCTGGTGTACATGGGCCACGCCATGCACA; CTGTGCGCATGCAGGAGAAGCGCAAGCTACGGGAGGCCGAGAGGGCCAAAGAGGTCCGGG; GCTCTGGCTCTTACGAGTACCCGGTGGCAGAGAAGGCAGAACTGTCCTGCTGGGAGGAAG; GGAATGGAAGGATTGCCCTCCAGGGCACTCTGCTCAACACCTATGTGTGCAGCATCCTGA; TCCGCACCACCATGGAGGTGGGCTTCATTGTGGGCCAGTACTTCATCTACGGAATCTTCC; TTGACACCCTGCATGTCTGCCGCAGGAGTCCCTGTCCCCACCCGGCAACTGGTACGTATC; CCGGCCCCAGAAAAAAAGTCTCCTTGTCTTTATGCGCTTGGGCTGCATTGTCCCTCTTCT; TAACTGGCTGACTCTACACCTTGGCTGGAAAAGACAGACGCCATTGTCCAACCCCGAGTA; CTTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005266 unedited; AGCGATAGCATTTATTAAGCCAAATTACACATTTTACTATATTTTTCCTTTCTAAAACCC; TCACCATCAAAGATTGATCCCACAGAATTTCTAATTCAAGAACACTCATTTCATAAAACA; TTTCAGAGATAGAAGGAGAGAAAATATTAATGCTAGGCAATAGATTAAAAGGTGTCCCCT; CCAGGGACCCGGGATGATCAGACATTCCTTCCTAAATGGCATCCTAGTCACTTTCCTCCA; CCTCTCCACTCTCATTTATCTTTCCACCCAACAATAGCATCCAAGTGTCAGCAGAGGGCC; CAGAGATGGGCAGGTGAGTCAGAGGTTGAACCTCCTTCTGAGCCTTTACCCTCCTCTGTC; CCAACCTCTTGTGGCTATCTGTTTAGGTTAATAACTGCCAGGCAGCCGGAGAGGAGAGCA; GGAAGACACAAGTTTCTCTGTGGTTTCCTTTCCTTATTTCAGGACTCCCCAAGCCTTTAC; CCATCCCATCAGCACCCACACACACACACACGTGCATTTTAATAATGTAATAATCTCTCT; ATAAGTCTCAGTTTCCAATTCCAAGCATCGTTTACCTTCATCCAAAACAACAGCAACCCA; TTATGGATCCAGTGAATACAGAGACCAGGCCAATCCCTGGGGAAGGCAAGGCTTTTCTAC; AGCCCCCACTTGGCAGTCTCATTAGGGGCACTAGGGAGACATAGGAATCTCTGCGCTCTT; TAAATAAGGCCCTGCCAAGAGAGCTGGAGATTCCCCTACTATNTCCTTGCCCCNTGCTAA; GCCTNCAGGAGAGAGAGATCTGGNGGCCTNCATAGCTGTCATCACACCTNCTCTTGGCAC; TAAGNTGGCAGTCAGCANAGGGAAGTAATAGGAGGGGAAAAATAGTTTCGNGGTTTCTCT; CATTNTATTTCATCTCCAGCAGATTTTTTTTTCACAGGGGATTGAGAGTCAAGCTTTCTT; GTTTACAAGCCTGGGAANTCACTGNCTCAGGCCTGAGACTGGCN;
• Restriction Sites: NotI-NotI
• ACCN: NM_005266
• Insert Size: 3740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005266.4, NP_005257.2
• RefSeq Size: 3201 bp
• RefSeq ORF: 3201 bp
• Locus ID: 2702
• UniProt ID: P36382
• Cytogenetics: 1q21.2
• Domains: CNX
• Protein Families: Transmembrane
• Summary: This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (A) represents the longer transcript. Variants A and B encode the same protein.