ATP dependent metalloprotease YME1L1 (YME1L1) (NM_139313) Human Untagged Clone

SKU SC108204

YME1L1 (untagged)-Human YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ATP dependent metalloprotease YME1L1
• Synonyms: FTSH, MEG4, PAMP, YME1L
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_139313, the custom clone sequence may differ by one or more nucleotides; ; ; ATGTTTTCCTTGTCGAGCACGGTGCAACCCCAGGTTACAGTTCCTCTGAGTCATCTCATCAATGCCTTCC; ATACACCAAAAAACACTTCTGTTTCTCTCAGTGGAGTGTCAGTTTCTCAAAACCAGCATCGAGATGTAGT; TCCTGAGCATGAGGCTCCCAGCAGTGAGTGTATGTTCAGTGACTTCCTGACGAAGCTTAACATTGTTTCA; ATTGGCAAAGGAAAAATATTCGAAGGGTACAGATCCATGTTCATGGAGCCAGCAAAAAGGATGAAGAAGA; GCTTGGACACAACCGATAACTGGCACATCCGTCCAGAACCCTTCTCCCTCTCAATCCCTCCTTCACTTAA; CTTAAGGGACCTTGGATTATCTGAACTAAAAATTGGACAGATTGATCAGCTGGTAGAAAATCTACTTCCT; GGATTTTGTAAAGGCAAAAACATTTCTTCCCATTGGCATACATCCCATGTCTCTGCACAATCCTTCTTTG; AAAATAAATATGGTAACTTAGATATATTTAGTACATTACGTTCCTCTTGCTTGTATCGACATCATTCAAG; AGCTCTTCAAAGCATTTGTTCAGATCTTCAGTACTGGCCAGTTTTCATACAGTCTCGGGGTTTTAAAACT; TTGAAATCAAGGACACGACGTCTCCAGTCTACCTCCGAGAGATTAGCTGAAACACAGAATATAGCGCCAT; CATTCGTGAAGGGGTTTCTTTTGCGGGACAGAGGATCAGATGTTGAGAGTTTGGACAAACTCATGAAAAC; CAAAAATATACCTGAAGCTCACCAAGATGCATTTAAAACTGGTTTTGCGGAAGGTTTTCTGAAAGCTCAA; GCACTCACACAAAAAACCAATGATTCCCTAAGGCGAACCCGTCTGATTCTCTTCGTTCTGCTGCTATTCG; GCATTTATGGACTTCTAAAAAACCCATTTTTATCTGTCCGCTTCCGGACAACAACAGGGCTTGATTCTGC; AGTAGATCCTGTCCAGATGAAAAATGTCACCTTTGAACATGTTAAAGGGGTGGAGGAAGCTAAACAAGAA; TTACAGGAAGTTGTTGAATTCTTGAAAAATCCACAAAAATTTACTATTCTTGGAGGTAAACTTCCAAAAG; GAATTCTTTTAGTTGGACCCCCAGGGACTGGAAAGACACTTCTTGCCCGAGCTGTGGCGGGAGAAGCTGA; TGTTCCTTTTTATTATGCTTCTGGATCCGAATTTGATGAGATGTTTGTGGGTGTGGGAGCCAGCCGTATC; AGAAATCTTTTTAGGGAAGCAAAGGCGAATGCTCCTTGTGTTATATTTATTGATGAATTAGATTCTGTTG; GTGGGAAGAGAATTGAATCTCCAATGCATCCATATTCAAGGCAGACCATAAATCAACTTCTTGCTGAAAT; GGATGGTTTTAAACCCAATGAAGGAGTTATCATAATAGGAGCCACAAACTTCCCAGAGGCATTAGATAAT; GCCTTAATACGTCCTGGTCGTTTTGACATGCAAGTTACAGTTCCAAGGCCAGATGTAAAAGGTCGAACAG; AAATTTTGAAATGGTATCTCAATAAAATAAAGTTTGATCAATCCGTTGATCCAGAAATTATAGCTCGAGG; TACTGTTGGCTTTTCCGGAGCAGAGTTGGAGAATCTTGTGAACCAGGCTGCATTAAAAGCAGCTGTTGAT; GGAAAAGAAATGGTTACCATGAAGGAGCTGGAGTTTTCCAAAGACAAAATTCTAATGGGGCCTGAAAGAA; GAAGTGTGGAAATTGATAACAAAAACAAAACCATCACAGCATATCATGAATCTGGTCATGCCATTATTGC; ATATTACACAAAAGATGCAATGCCTATCAACAAAGCTACAATCATGCCACGGGGGCCAACACTTGGACAT; GTGTCCCTGTTACCTGAGAATGACAGATGGAATGAAACTAGAGCCCAGCTGCTTGCACAAATGGATGTTA; GTATGGGAGGAAGAGTGGCAGAGGAGCTTATATTTGGAACCGACCATATTACAACAGGTGCTTCCAGTGA; TTTTGATAATGCCACTAAAATAGCAAAGCGGATGGTTACCAAATTTGGAATGAGTGAAAAGCTTGGAGTT; ATGACCTACAGTGATACAGGGAAACTAAGTCCAGAAACCCAATCTGCCATCGAACAAGAAATAAGAATCC; TTCTAAGGGACTCATATGAACGAGCAAAACATATCTTGAAAACTCATTCTTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139313 unedited; GGGGGTTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGGGAG; GCCATTTTCCGTTTCTGGGAGGAGTGAGGGGCAACGGGTCGGAGAAAAAGGAAAAAAGAA; GGGCTCAGCGCCTCCCCGCCGGGCCGTGGACAGAGGGGCACAGTTTCGGCAGGCGGGTGA; GGTCGCTGAGGGCCCGCCGGAGATGTTTTCCTTGTCGAGCACGGTGCAACCCCAGGTTAC; AGTTCCTCTGAGTCATCTCATCAATGCCTTCCATACACCAAAAAACACTTCTGTTTCTCT; CAGTGGAGTGTCAGTTTCTCAAAACCAGCATCGAGATGTAGTTCCTGAGCATGAGGCTCC; CAGCAGTGAGTGTATGTTCAGTGACTTCCTGACGAAGCTTAACATTGTTTCAATTGGCAA; AGGAAAAATATTCGAAGGGTACAGATCCATGTTCATGGAGCCAGCAAAAAGGATGAAGAA; GAGCTTGGACACAACCGATAACTGGCACATCCGTCCAGAACCCTTCTCCCTCTCAATCCC; TCCTTCACTTAACTTAAGGGACCTTGGATTATCTGAACTAAAAATTGGACAGATTGATCA; GCTGGTAGAAAATCTACTTCCTGGATTTTGTAAAGGCAAAAACATTTCTTCCCATTGGCA; TACATCCCATGTCTCTGCACAATCCTTCTTTGAAAATAAATATGGTAACTTAGATATATT; TAGTACATTACGTTCCTCTTGCTTGTATCGACATCATTCAAGAGCTCTTCAAGCATTTGT; TCAGATCTTCAGTACTGGCCAGCATTAATTTGGAGATTCTGNACAATATGATTTAAAAGA; AAACGAAATGAGCCATATTTTTCATACAGNTCTCGGGGTTTTTAAAACTTTNGAATCANG; GACACGACGTCTTCAGTCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_139313 unedited; NAAATNCGAGTTTTTTTTTTTTTTTTTTTTTTGTTTTCAAACCTGATAGTTCTTTATTTG; GGAAAATATATTTGCCATGGGATGCTAATTTGCAATAGGTGTCATAATGAGAATAACCCA; AACTGGATAAATGTGACAAATGATTGACAAAGCATTTCACACCCTTCAATTACACCACAT; CAAGAATGAGGGGAAAGCGTTGTAAAAGTAGACTACTGGGATGCTACTTATATTCTTGCA; ATAAAACCAGCAAGCATCCATATCAAGAGAGTTATCATCTCACTTCCAACTTTTTCCCCT; CAAGAACAATTTGAATCTCTTTGGCATCCAAAGTCTCATAGGTCAATAAAGCTTCTGCGA; GATTCTTATGCTCCTTTGCATGAGTTTTCAAGATATGTTTTGCTCGTTCATATGAGTCCC; TTAAAAAGATTCTTATTTCTTGTTCGATGGCAGATTGGGTTTCTGGACTTAGTTTCCCTG; TATCACTGTAGGTCATAACTCCAAGCTTTTCACTCATTCCAAATTTGGTAACCATCCGCT; TTGCTATTTTAGTGGCATTATCAAAATCACTGGAAGCACCTGTTGTAATATGGTCGGTTC; CAAATATAAGCTCCTCTGCCACTCTTCCTCCCATACTAACATCCATTTGTGCAAGCAGCT; GGGCCTCTATTTCATTCCATCTGTCATTCTCAGGTAACAGGGACACATGTCCAAATGTTG; GCCCCCGTGGCATGATTGTACCTTTGTTGGAAGGCATTGCATCTTTTGTGTAATATGCAA; AAAGGGCTGACCAAATTTCTGATATGCTGGGATGCTTTTGCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_139313
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_139313.1, NP_647474.1
• RefSeq Size: 2584 bp
• RefSeq ORF: 2223 bp
• Locus ID: 10730
• Cytogenetics: 10p12.1
• Domains: AAA, Peptidase_M41
• Protein Families: Druggable Genome, Protease
• Summary: The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]; Transcript Variant: This variant (2) is missing a segment of the coding region at the 3' end compared to transcript variant 1. This results in an isoform (2) that is shorter at the C-terminus compared to isoform 1.