FGF12 (NM_004113) Human Untagged Clone

SKU SC108203

FGF12 (untagged)-Human fibroblast growth factor 12 (FGF12), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: FGF12
• Synonyms: DEE47; EIEE47; FGF12B; FHF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108203 sequence for NM_004113 edited (data generated by NextGen Sequencing); ATGGAGAGCAAAGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAGCAGGGA; TACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAACAGCGAC; TACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGAGTGAAG; GCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTCAGATGTTTTCACT; CCAGAATGCAAATTCAAGGAATCTGTGTTTGAAAACTACTATGTGATCTATTCTTCCACA; CTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGTTTCTGGGACTCAATAAAGAAGGTCAA; ATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTACCGAAACCT; ATTGAAGTGTGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAAACAAGGGCGT; TCAAGGAAAAGTTCTGGAACACCAACCATGAATGGAGGCAAAGTTGTGAATCAAGATTCA; ACATAG; ; Clone variation with respect to NM_004113.5;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004113 unedited; NCCGTTCAAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGTCACT; ACACCTGCAGCACCACGCTTCGGAGACAGCTCCTGCCTGCAGCTTCCAGACCCAGGAAGC; CTGAGGGGAAGGAAGGAAGTACGGGCGAAATCATCAGATTGGCTTCCCAGATTTGGGAAT; CTGAAGCGGGCCCACATCTTCCGGCCAACTTCCATTGAACTTCCCAGCACTCGAAAGGGA; CCGAAATGGAGAGCAAAGAACCCCAGCTCAAAGGGATTGTGACAAGGTTATTCAGCCAGC; ANGGATACTTCCTGCAGATGCACCCAGATGGTACCATTGATGGGACCAAGGACGAAAACA; GCGACTACACTCTCTTCAATCTAATTCCCGTGGGCCTGCGTGTAGTGGCCATCCAAGGAG; TGAAGGCTAGCCTCTATGTGGCCATGAATGGTGAAGGCTATCTCTACAGTTNCAGATGTT; TTCACTCCAGAATGCAAATTCAAGGAATCTGGTGTTTGAAAACTACNTATGTGATCTATT; CTTCCACACTGTACCGCCAGCAAGAATCAGGCCGAGCTTGGNTTTCTGGGACTCAATAAA; GAAGGTCAAATTATGAAGGGGAACAGAGTGAAGAAAACCAAGCCCTCATCACATTTTGTA; CCGAAACTATTTGAAGTGGGGTATGTACAGAGAACCATCGCTACATGAAATTGGAGAAAA; CCAAGGGCGTTAGGGGGAAAGGTTCTGGGACACCACCATGAATGAAGGCNNAAGTTGTGA; ATCAAGATTCACATAGCTGANGACTCTCCCTTCNTCCTCTCTCATCCCTTCCCCTTCCCT; TCNTTCCATTTACCCATTTNCTTNCCGTANATCACCCAGGGAGAGGAAAATAAATGACAC; GCAGGACTAGGGCTTAAAATCTGCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004113 unedited; GCTATGGACCGCGNGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGTTCTGGT; AGTGACAGGTGTATTTATAATCAAGTTGAATCAAGAGTGACAAGAAGAAATACAGCTAGA; GTTATATTTTTGCCCCAGGGGTATTCTTTTCCTAGAAGAGCAAGTCCATTTTTAGAAAAT; TTAAATGTCTTTATTTGTTACTTTCCAAATATTTTGGTTAAACAAATATCTCTTGCAAAT; GTATCTTCAAAATCTTTGCCTACATGCATACAATTTGTTCTTCCCAACTGCTTAGGGAAA; TTCCTTCATTTCATTTTCTGCTAACCTCTAGATAACAGAAATAGGATACCTGGTGAAGGA; TATGGTCCACTATTGAAGAATAATGATTGTGTAAAGTGAGGGAAACTATATTGACCTTGA; AATACGGCAACCAATGGCAAGGTTTGAGGTGAGGGCCTAGAGCTTCTGCAAAGTGTATAT; ATTTTAGGGAGTCATATTTGGAACAATACATGCTGGACAGTAGAGATATAGGAGAAGTTA; CATGCTATGTCTTCTCATTGATAATATCCATAAATCTGCATACATTTTTAACATTCTGTT; TTGGGGGTTTTTTTTCTTTAAATNTGGNATGTCTCTACACCACTCCTGATTTGTAGGACT; AAATAGATCTATTTATTCCAATGCAAATTGTGTAACATTTATTTCTTCCTTGATTTTTAA; AAATACTTTAGTATTCTTAACTAATGTATGTGCCTTCTCTTACACTGAGTNCTTTTTTTG; CTCTTTCAGCTGCTACACAATCCTGTCTGGTGGAGTCATAGCTGCTAATCCCATATATTT; CTCTACACACCTTGACATTNANGNATTACTGGAACCCTTCTGTGTCTAGGAACAAATGAT; TGCAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_004113
• Insert Size: 2940 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004113.3, NP_004104.3
• RefSeq Size: 2861 bp
• RefSeq ORF: 546 bp
• Locus ID: 2257
• UniProt ID: P61328
• Cytogenetics: 3q28-q29
• Domains: FGF
• Protein Families: Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton
• Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. [provided by RefSeq, Dec 2019]; Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (2) contains a distinct and shorter C-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.