BAD (NM_004322) Human Untagged Clone

SKU SC107955

BAD (untagged)-Human BCL2-associated agonist of cell death (BAD), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: BAD
• Synonyms: BBC2; BCL2L8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004322 edited; ATGTTCCAGATCCCAGAGTTTGAGCCGAGTGAGCAGGAAGACTCCAGCTCTGCAGAGAGG; GGCCTGGGCCCCAGCCCCGCAGGGGACGGGCCCTCAGGCTCCGGCAAGCATCATCGCCAG; GCCCCAGGCCTCCTGTGGGACGCCAGTCACCAGCAGGAGCAGCCAACCAGCAGCAGCCAT; CATGGAGGCGCTGGGGCTGTGGAGATCCGGAGTCGCCACAGCTCCTACCCCGCGGGGACG; GAGGACGACGAAGGGATGGGGGAGGAGCCCAGCCCCTTTCGGGGCCGCTCGCGCTCGGCG; CCCCCCAACCTCTGGGCAGCACAGCGCTATGGCCGCGAGCTCCGGAGGATGAGTGACGAG; TTTGTGGACTCCTTTAAGAAGGGACTTCCTCGCCCGAAGAGCGCGGGCACAGCAACGCAG; ATGCGGCAAAGCTCCAGCTGGACGCGAGTCTTCCAGTCCTGGTGGGATCGGAACTTGGGC; AGGGGAAGCTCCGCCCCCTCCCAGTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004322 unedited; NTTTCACGATTTGTATACGATTCATATAGGCGGCCGCGNAATTCGCACGAGGGGGAGGCG; GCAGGCCCGGGTCAGGGGCCTCGAGATCGGGCTTGGGCCCAGAGCATGTTCCAGATCCCA; GAGTTTGAGCCGAGTGAGCAGGAAGACTCCAGCTCTGCAGAGAGGGGCCTGGGCCCCAGC; CCCGCAGGGGACGGGCCCTCAGGCTCCGGCAAGCATCATCGCCAGGCCCCAGGCCTCCTG; TGGGACGCCAGTCACCAGCAGGAGCAGCCAACCAGCAGCAGCCATCATGGAGGCGCTGGG; GCTGTGGAGATCCGGAGTCGCCACAGCTCCTACCCCGCGGGGACGGAGGACGACGAAGGG; ATGGGGGAGGAGCCCAGCCCCTTTCGGGGCCGCTCGCGCTCGGCGCCCCCCAACCTCTGG; GCAGCACAGCGCTATGGCCGCGAGCTCCGGAGGATGAGTGACGAGTTTGTGGACTCCTTT; AAGAAGGGACTTCCTCGCCCGAAGAGCGCGGGCACAGCAACGCAGATGCGGCAAAGCTCC; AGCTGGACGCGAGTCTTCCAGTCCTGGTGGGATCGGAACTTGGGCAGGGGAAGCTCCGCC; CCCTCCCAGTGACCTTCGCTCCACATCCCGAAACTCCACCCGTTCCCACTGCCCTGGGCA; GCCATCTTGAATATGGGCGGAAGTACTTCCCTCAGGCCTATGCAAAAAGAGGATCCGTGC; TGTCTCCTTTGGAGGGAGGGCTGACCCAGATTCCCTTCCCGTGCGTGTGAAGCCCCCGGA; AGCTTGGTCCCATCCGAAGTTATGGGTTTTCCGCCCCCAGCCGCCGGTAGTGGCTCCGTG; GCCCCGCCCTCAGCTTCCGGCTTTCCCCCAGCGCCTGCCCTAATTGCGAGCCAGGTTTAC; CGTTGTGTACCGGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004322 unedited; GCCCTCGATAGTCGAGTTTTTTTTTTTTTTTTTTTTGCACAAACGCGGTCTTTATTAACA; TTTGGTAGTGAGCACGGCCCCCAGGGCATCGCGGGGGCTCGGGTCCCGGTGACGCAACGG; TTAAACCTGGCTCGCGACTTAACGCAGGCGCCTGGGGGAAAGCCCGGAGCCTGAGGGCGG; GGCCACGGAGCCACTTCCGGCGGCTGTGGGCGGAAAACCCAAAACTTCCGATGGGACCAA; GCCTTCCGTGGCTTCACACGCACCGGAAGGGAATCTGGGTCAGCCCTCCCTCCAAAGGAG; ACAGCACGGATCCTCTTTTTGCATAGGCCTGAGGGAAGTACTTCCGCCCATATTCAAGAT; GGCTGCCCAGGGCAGTGGGAACGGGTGGAGTTTCGGGATGTGGAGCGAAGGTCACTGGGA; GGGGGCGGAGCTTCCCCTGCCCAAGTTCCGATCCCACCAGGACTGGAAGACTCGCGTCCA; GCTGGAGCTTTGCCGCATCTGCGTTGCTGTGCCCGCGCTCTTCGGGCGAGGAAGTNCCTT; CTTAAAGGAGTCCACAAACTCGTCACTCATCCTCCGGAGCTCGCGGCCATAGCGCTGTGC; TGCCCANAGTTGGGGGGCGCCNAGCGCGAGCGGCCCCNAAAGGGGCTGGGCTCCTCCCCA; TCCCTTTGTCGTCCTCCGTCCCCGCGGGGTATGAGCTGTGGCGACTTCCGATCTCACAGC; CCCAGCGCCTCATGATGGCTGCTGCTGGNTGGCTGCTCCTGCTGGTGACTGGCGTCCACA; CGAAGCCTGNGGCCCTGCGATGATTCTTGCCGAAACCTGAGGCCCCTGTCCCTGTCGGGC; TGGGGCCCAAGCCCCCTCTTGCAAGCTGAATCTTCTGCTACTCGCTCAACTTTGGGACTG; AACATGTCTGGGCCAACCCGATTTGAGGCCTGAACGGNCTGCCCCTCCCTTTGCAGAATC; GGGCCCCCTTAAGAGTGTTACAAATTGAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004322
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004322.2, NP_004313.1
• RefSeq Size: 1127 bp
• RefSeq ORF: 507 bp
• Locus ID: 572
• UniProt ID: Q92934
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Insulin signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, VEGF signaling pathway
• Summary: The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Dec 2019]; Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.