PAK1 (NM_002576) Human Untagged Clone

SKU SC107950

PAK1 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: PAK1
• Synonyms: alpha-PAK; IDDMSSD; p65-PAK; PAKalpha
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002576 edited; TCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTA; TATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCG; GCCGCGAATTCGGCACGAGGCACCGCCGGGAGGAGGAGGAGGAGCCGAGAGGAGCTGAGC; GAGCGCGGAAGTAGCTGCTGCTGGTGGTGACAATGTCAAATAACGGCCTAGACATTCAAG; ACAAACCCCCAGCCCCTCCGATGAGAAATACCAGCACTATGATTGGAGCCGGCAGCAAAG; ATGCTGGAACCCTAAACCATGGTTCTAAACCTCTGCCTCCAAACCCAGAGGAGAAGAAAA; AGAAGGACCGATTTTACCGATCCATTTTACCTGGAGATAAAACAAATAAAAAGAAAGAGA; AAGAGCGGCCAGAGATTTCTCTCCCTTCAGATTTTGAACACACAATTCATGTCGGTTTTG; ATGCTGTCACAGGGGAGTTTACGGGAATGCCAGAGCAGTGGGCCCGCTTGCTTCAGACAT; CAAATATCACTAAGTCGGAGCAGAAGAAAAACCCGCAGGCTGTTCTGGATGTGTTGGAGT; TTTACAACTCGAAGAAGACATCCAACAGCCAGAAATACATGAGCTTTACAGATAAGTCAG; CTGAGGATTACAATTCTTCTAATGCCTTGAATGTGAAGGCTGTGTCTGAGACTCCTGCAG; TGCCACCAGTTTCAGAAGATGAGGATGATGATGATGATGATGCTACCCCACCACCAGTGA; TTGCTCCACGCCCAGAGCACACAAAATCTGTATACACACGGTCTGTGATTGAACCACTTC; CTGTCACTCCAACTCGGGACGTGGCTACATCTCCCATTTCACCTACTGAAAATAACACCA; CTCCACCAGATGCTTTGACCCGGAATACTGAGAAGCAGAAGAAGAAGCCTAAAATGTCTG; ATGAGGAGATCTTGGAGAAATTACGAAGCATAGTGAGTGTGGGCGATCCTAAGAAGAAAT; ATACACGGTTTGAGAAGATTGGACAAGGTGCTTCAGGCACCGTGTACACAGCAATGGATG; TGGCCACAGGACAGGAGGTGGCCATTAAGCAGATGAATCTTCAGCTAAAGAGCTGCTACA; GCATCAATTCCTGAAGATTGCCAAGCCCCTCTCCAGCCTCACTCCACTGATTGCTGCAGC; TAAGGAGGCAACAAAGAACAATCACTAAAACCACACTCACCCCAGCCTCATTGTGCCAAG; CCTTCTGTGAGATAAATGCACATTTCAGAAATTCCAACTCCTGATGCCCTCTTCTCCTTG; CCTTGCTTCTCCCATTTCCTGATCTAGCACTCCTCAAGACTTTGATCCTTGGAAACCGTG; TGTCCAGCATTGAAGAGAACTGCAACTGAATGACTAATCAGATGATGGCCATTTCTAAAT; AAGGAATTTCCTCCCAATTCATGGATATGAGGGTGGTTTATGATTAAGGGTTTATATAAA; TAAATGTTTCTAGTCTTCCGTGTGTCAAAATCCTCACCTCCTTCATAACCATCTCCCACA; ATTAATTCTTGACTATATAAATTTATGGTTTGATAATATTATCAATTTGTAATCAATTGA; GATTTCTTTAGTGCTTGCTTTTCTGTGACTCAACTGCCCAGACACCTCATTGTACTTGAA; AACTGGAACAGCTTGGGAATGCCATGGGGTTTGATAATCTGCCAGGGACATGAAGAGGCT; CAGCTTCCTGGACCATGACTTTGGCTCAGCTGATCCTGACATGGGAGAACAACCACATTT; TTCTTTGTGTGTGCTTCTAGCAGCTGTTCGGGAGGACCTTGACCCAATAGTGTTCCCATG; CTGTTTCTTGTGAAATGCTCTCGGCTATGTAGCAGCTTTTGATTCCCTGCATACCCTAGG; CTGCTGCCCCTATCCTGTCCCTTGTTTATAACATTGAGAGGTTTTCTAGGGCACATACTG; AGTGAGAGCAGTGTTGAGAAGTCGGGGAAAATGGTGACTACTTTTAGAGCAAGGCTGGGC; ATCAGCACCTGTCCAGCTCTACTTGTGTGATGTTTCAGGAACTCAGCCCCTTTTTCTGCC; TAGGATAAGGAGCTGAAAGATTAACTTGGATCTTCTAATGGTCCAAATCTTTTGGTCACA; ATAAAGAGTCTCCAAATTAGAGACTGCATGTTAGTTCTGGATGGATTTGGTGGCCTGACA; TGATACCCTGCCAGCTGTGAGGGGACCCCGTTTTTAAGATGCATGGCCAAGCTCTCTGCA; AATGGAAATGCTTACACTGGGTGTTGGGGATGTTTGCTACCTCCTGCTATTTTTGTGGTT; TTGGTTCTCCCACTATGGTAGGACCCCTGGCCAGCATTGTGGCTTGTCATGTCAGCCCCA; TTGACTACCTTCTCATGCT;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002576 unedited; CGCCAGGAATTTGTAACACACTCACTATAGGCGGCCGCGCAATTCGCACGAGCACCGCCG; GGAGGAGGAGGAGGAGCCGAGAGGAGCTGAGCGAGCGCGGAAGTAGCTGCTGCTGGTGGT; GACAATGTCAAATAACGGCCTAGACATTCAAGACAAACCCCCAGCCCCTCCGATGAGAAA; TACCAGCACTATGATTGGAGCCGGCAGCAAAGATGCTGGAACCCTAAACCATGGTTCTAA; ACCTCTGCCTCCAAACCCAGAGGAGAAGAAAAAGAAGGACCGATTTTACCGATCCATTTT; ACCTGGAGATAAAACAAATAAAAAGAAAGAGAAAGAGCGGCCAGAGATTTCTCTCCCTTC; AGATTTTGAACACACAATTCATGTCGGTTTTGATGCTGTCACAGGGGAGTTTACGGGAAT; GCCAGAGCAGTGGGCCCGCTTGCTTCAGACATCAAATATCACTAAGTCGGAGCAGAAGAA; AAACCCGCAAGCTGTTCTGGATGTGTTGGAGTTTTACAACTCGAAGAAGACATCCAACAG; CCAGAAATACATGAGCTTTACAGATAAGTCAGCTGAGGATTACAATTCTTCTAATGCCTT; GAATGTGAAGGCTGTGTCTGAGACTCCTGCAGTGCCACCAGTTTCAGAAGATGAGGATGA; TGATGATGATGATGCTACCCCACCACCAGTGATTGCTCCACGCCCAGAGCACACAAAATC; TGTATACACACGGTCTGTGATTGAACCACTTCCTGTCACTCCAACTCGGGACGTGGCTAC; ATCTCCCATTTCACCTACTGAAAATAACACCACTCCACCAGATGCTTTGACCCGGAATAC; TGAGAAGCAGAAGAAGAAGCCCTANATGTCTGACTGAGAGATCTTGGAGAATTACGAAGC; TAGTGAGTGTGGGCGATCCTAAGAAGAATATACACGGTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002576 unedited; CCCCAATTAGTTTGNACCGTCGGCCGCATCCTACGAGCGAGTTTTTTTTTTTTTTTTTTT; TTTTTATTTTCATCTCCTTTTATTGACAGAAATAGAAATTTGTGCTGCAGAGGCAGTAGT; ACCTCAGAGCATGAGAAGGTAGTCAATGGGGCTGACATGACAAGCCACAATGCTGGCCAG; GGGTCCTACCATAGTGGGAGAACCAAAACCACAAAAATAGCAGGAGGTAGCAAACATCCC; CAACACCCAGTGTAAGCATTTCCATTTGCAGAGAGCTTGGCCATGCATCTTAAAAACGGG; GTCCCCTCACAGCTGGCAGGGTATCATGTCAGGCCACCAAATCCATCCAGAACTAACATG; CAGTCTCTAATTTGGAGACTCTTTATTGTGACCAAAAGATTTGGACCATTAGAAGAGCCA; AGTTAATCTTTCAGCTCCTTATCCTAGGCAGAAAAAGGGGCTGAGTTCCTGAAACATCAC; ACAAGTAGAGCTGGACAGGTGCTGATGCCCAGCCTTGCTCTAAAAGTAGTCACCATTTTC; CCCGACTTCTCAACACTGCTCTCACTCAGTATGTGCCCTAGAAAACCTCTCAATGTTATA; AACAAGGGACAGGATAGGGGCAGCAGCCTAGGGTATGCAGGGAATCAAAAGCTGCTACAT; AGCCTAGAGCATTTCACAAGAAACAGCATGGGAACACTATTGGGTCAAGGTCCTTCCGAA; CAGCTGCTAGAAGCACACACAAAGAACAATGTGGTTGTTCTCCCATGTCAGGATCAGCTG; AGCCAAAGTCATGGTCCAGGAAGCTGAGCCTCTTCATGTCCCTGGCAGATATCAAACCCC; ATGCCATTCCAAGCTGTTCCAGTTTTCAAGTCAATGAGTGTCTGGGCACATTGATCCAGA; AC;
• Restriction Sites: NotI-NotI
• ACCN: NM_002576
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002576.3, NP_002567.3
• RefSeq Size: 3264 bp
• RefSeq ORF: 1638 bp
• Locus ID: 5058
• UniProt ID: Q13153
• Cytogenetics: 11q13.5-q14.1
• Domains: PBD, pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
• Summary: This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Mutations in this gene have been associated with macrocephaly, seizures, and speech delay. Overexpression of this gene is also reported in many cancer types, and particularly in breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2020]; Transcript Variant: This variant (2) lacks a coding exon in the 3' region, as compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.