Cytochrome C Oxidase subunit VIb (COX6B1) (NM_001863) Human Untagged Clone

SKU SC107931

COX6B1 (untagged)-Human cytochrome c oxidase subunit VIb polypeptide 1 (ubiquitous) (COX6B1), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cytochrome C Oxidase subunit VIb
• Synonyms: COX6B; COXG; COXVIb1; MC4DN7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001863 edited; ATGGCGGAAGACATGGAGACCAAAATCAAGAACTACAAGACCGCCCCTTTTGACAGCCGC; TTCCCCAACCAGAACCAGACTAGAAACTGCTGGCAGAACTACCTGGACTTCCACCGCTGT; CAGAAGGCAATGACCGCTAAAGGAGGCGATATCTCTGTGTGCGAATGGTACCAGCGTGTG; TACCAGTCCCTCTGCCCCACATCCTGGGTCACAGACTGGGATGAGCAACGGGCTGAAGGC; ACGTTTCCCGGGAAGATCTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001863 unedited; CAGTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGACTGT; GGTGTCTTTGCTGAGGGTCACATTGAGCTGCAGGTTGAATCCGGGGTGCCTTTAGGATTC; AGCACCATGGCGGAAGACATGGAGACCAAAATCAAGAACTACAAGACCGCCCCTTTTGAC; AGCCGCTTCCCCAACCAGAACCAGACTAGAAACTGCTGGCAGAACTACCTGGACTTCCAC; CGCTGTCAGAAGGCAATGACCGCTAAAGGAGGCGATATCTCTGTGTGCGAATGGTACCAG; CGTGTGTACCAGTCCCTCTGCCCCACATCCTGGGTCACAGACTGGGATGAGCAACGGGCT; GAAGGCACGTTTCCCGGGAAGATCTGAACTGGCTGCATCTCCCTTTCCTCTGTCCTCCAT; CCTTCTCCCAGGATGGTGAAGGGGGACCTGGTACCCAGTGATCCCCACCCCAGGATCCTA; AATCATGACTTACCTGCTAATAAAAACTCATTGGAAAAAAAAAAAAAAAAAACTCGACTC; TAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGAC; CCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTC; CTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTTAATATTATGGGGT; GAGGGNGGGGGTTTGTGACACCAGGGGCAAAATTGGGAAAAAAACCTTGTGGGCCTGGCG; GGGGTCTATGGGGACCACACTGGAGGGGAGTGGACCAATCCTGCTCACTGGAATCTCCGC; CTCCTGGGGCCAAGCGATTCTCCTGCCTCAGCTCCCGGGTTGGTTGGGTATTCCAGGCAT; GCATGACCAGCCTAGCTAATTTTGGTTTTTTGGTAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001863 unedited; CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCAATGAGTTTTTATTAG; CAGGTAAGTCATGATTTAGGATCCTGGGGTGGGGATCACTGGGTACCAGGTCCCCCTTCA; CCATCCTGGGAGAAGGATGGAGGACAGAGGAAAGGGAGATGCAGCCAGTTCAGATCTTCC; CGGGAAACGTGCCTTCAGCCCGTTGCTCATCCCAGTCTGTGACCCAGGATGTGGGGCAGA; GGGACTGGTACACACGCTGGTACCATTCGCACACAGAGATATCGCCTCCTTTAGCGGTCA; TTGCCTTCTGACAGCGGTGGAAGTCCAGGTAGTTCTGCCAGCAGTTTCTAGTCTGGTTCT; GGTTGGGGAAGCGGCTGTCAAAAGGGGCGGTCTTGTAGTTCTTGATTTTGGTCTCCATGT; CTTCCGCCATGGTGCTGAATCCTAAAGGCACCCCGGATTCAACCTGCAGCTCAATGTGAC; CCTCAGNCAAGACACCACAGTCGGCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTC; GTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTAATAGACCTCCCACCGTAC; ACGCCTACCGCCCATTTGCGTCAACGGCGCGGGGTTATTACCACATTTTGGAAAGCCCGT; TGATTTTGGTGCCACAACAAACTCCCATTGACGTCAAGGGGGTGGAGACCTTGAAATCCC; CGTGAGTCAAACCGCTCTCCCCGCCCATCGGTGTCCTGCCAAAACCCCATCACCTGGGAA; TACCCAGGACTAATCCCCAGTGAACCGCCAAGCCGAAACGCCCCGAGGGCCATGCCTGGG; CCTAAGACCGGCCGCCCTTTCCCGCATGTCCCAATGGGGGCGGCCTGGCTATGACCCTTG; ACGCCCGCAGCGGCCCCTACCGTAAACTCCCCCTGACGCAGGAAAGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001863
• Insert Size: 560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001863.3, NP_001854.1
• RefSeq Size: 578 bp
• RefSeq ORF: 261 bp
• Locus ID: 1340
• UniProt ID: P14854
• Cytogenetics: 19q13.12
• Domains: COX6B
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Summary: Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIb. Mutations in this gene are associated with severe infantile encephalomyopathy. Three pseudogenes COX6BP-1, COX6BP-2 and COX6BP-3 have been found on chromosomes 7, 17 and 22q13.1-13.2, respectively. [provided by RefSeq, Jan 2010]