TMEM65 (NM_194291) Human Untagged Clone

CAT#: SC107673

TMEM65 (untagged)-Human transmembrane protein 65 (TMEM65)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TMEM65
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_194291, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCCGGCTGCTGCCGCTGCTGAGGAGCCGGACCGCGCGCAGCCTGAGGCCGGGCCCGGCCGCCGCCG
  CCGCGCCCCGCCCGCCGTCCTGGTGCTGCTGCGGGCGGGGGCTGCTGGCGCTCGCGCCCCCCGGCGGCTT
  GCCGGGCGGCCCCAGGCGGCTGGGCACGCACCCCAAGAAGGAGCCCATGGAGGCGCTGAACACGGCGCAG
  GGCGCGCGCGACTTCATCTACAGCCTGCACTCCACGGAGAGGAGCTGCCTGCTCAAAGAGCTGCACCGCT
  TCGAGTCTATTGCCATTGCCCAAGAAAAATTGGAAGCTCCACCACCCACCCCAGGACAGCTGAGATATGT
  ATTCATCCACAATGCGATACCTTTCATAGGGTTTGGCTTTTTGGATAATGCAATTATGATTGTTGCTGGA
  ACCCATATTGAAATGTCTATTGGAATTATTTTGGGAATTTCAACTATGGCAGCTGCTGCTTTGGGAAATC
  TTGTGTCAGATCTAGCTGGACTTGGACTTGCAGGCTACGTTGAAGCATTGGCTTCCAGGTTAGGCCTGTC
  AATTCCTGATCTCACACCAAAGCAAGTTGACATGTGGCAAACACGTCTTAGTACACATTTGGGCAAAGCT
  GTTGGGGTGACTATTGGCTGCATTCTAGGAATGTTTCCTTTAATTTTCTTTGGAGGAGGTGAAGAAGATG
  AAAAACTGGAAACGAAAAGTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_194291 unedited
  CCCTGTTCGNAATTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGCGGGCCC
  GCGGAGTGCATGGTGCCCGGCGCCTCGGCTGCCTGGCAGGAGGACCTCGGGGCGGGGTCG
  AGCTGAGCCCAGCTCCTTCTCGCCTCAGCCGCGCCAGAAACCGCCTTCCCGGACGGCCGC
  GGGGGTCCCAGCTCCTCAGCTCGCCATGTCCCGGCTGCTGCCGCTGCTGAGGAGCCGGAC
  CGCGCGCAGCCTGAGGCCGGGCCCGGCCGCCGCCGCCGCGCCCCGCCCGCCGTCCTGGTG
  CTGCTGCGGGCGGGGGCTGCTGGCGCTCGCGCCCCCCGGCGGCTTGCCGGGCGGCCCCAG
  GCGGCTGGGCACGCACCCCAAGAAGGAGCCCATGGAGGCGCTGAACACGGCGCAGGGCGC
  GCGCGACTTCATCTACAGCCTGCACTCCACGGAGAGGAGCTGCCTGCTCAAAGAGCTGCA
  CCGCTTCGAGTCTATTGCCATTGCCCAAGAAAAATTGGAAGCTCCACCACCCACCCCAGG
  ACAGCTGAGATATGTATTCATCCACAATGCGATACCTTTCATAGGGTTTGGCTTTTTGGA
  TAATGCAATTATGATTGTTGCTGGAACCCATATTGAAATGTCTATTGGAATTATTTTGGG
  AATTTCAACTATGGCAGCTGCTGCTTTGGGAAATCTTGTGTCAGATCTAGCTGGACTTGG
  ACTTGCAGGCTACGTTGAAGCATTGGCTTCCAGGTTAGGCCTGTCAATTCCTGATCTCCA
  CCANAGCAGTTGACATGTGGCAACACGTCTTATACACATTTGGGCAAGCTGTTGGGGTGA
  CTATTGGCTGCATCTAGGAATGGTCCTTTAATTTCTTGGAGGAGGTGAGAGATGAAACTG
  GAACGANAGNTATCCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_194291
• Insert Size: 3950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194291.1, NP_919267.1
• RefSeq Size: 3258 bp
• RefSeq ORF: 723 bp
• Locus ID: 157378
• UniProt ID: Q6PI78
• Cytogenetics: 8q24.13
• Protein Families: Transmembrane
• Gene Summary: May play an important role in cardiac development and function. May regulate cardiac conduction and the function of the gap junction protein GJA1. May contributes to the stability and proper localization of GJA1 to cardiac intercalated disk thereby regulating gap junction communication (By similarity). May also play a role in the regulation of mitochondrial respiration and mitochondrial DNA copy number maintenance (PubMed:28295037).[UniProtKB/Swiss-Prot Function]