MNK1 (MKNK1) (AB000409) Human Untagged Clone

SKU SC107659

(untagged)-Human mRNA for MNK1, complete cds

Product Data

• Type: Human Untagged Clone
• Target Symbol: MNK1
• Synonyms: MNK1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for AB000409 edited; ATGGTATCTTCTCAAAAGTTGGAAAAACCTATAGAGATGGGCAGTAGCGAACCCCTTCCC; ATCGCAGATGGTGACAGGAGGAGGAAGAAGAAGCGGAGGGGCCGGGCCACTGACTCCTTG; CCAGGAAAGTTTGAAGATATGTACAAGCTGACCTCTGAATTGCTTGGAGAGGGAGCCTAT; GCCAAAGTTCAAGGTGCCGTGAGCCTACAGAATGGCAAAGAGTATGCCGTCAAAATCATC; GAGAAACAAGCAGGGCACAGTCGGAGTAGGGTGTTTCGAGAGGTGGAGACGCTGTATCAG; TGTCAGGGAAACAAGAACATTTTGGAGCTGATTGAGTTCTTTGAAGATGACACAAGGTTT; TACTTGGTCTTTGAGAAATTGCAAGGAGGTTCCATCTTAGCCCACATCCAGAAGCAAAAG; CACTTCAATGAGCGAGAAGCCAGCCGAGTGGTGCGGGACGTTGCTGCTGCCCTTGACTTC; CTGCATACCAAAGGCATTGCTCATCGTGATCTGAAACCAGAAAATATATTGTGTGAATCT; CCAGAAAAGGTGTCTCCAGTGAAAATCTGTGACTTTGACTTGGGCAGTGGGATGAAACTG; AACAACTCCTGTACCCCCATAACCACACCAGAGCTGACCACCCCATGTGGCTCTGCAGAA; TACATGGCCCCTGAGGTAGTGGAGGTCTTCACGGACCAGGCCACATTCTACGACAAGCGC; TGTGACCTGTGGAGCCTGGGCGTGGTCCTCTACATCATGCTGAGTGGCTACCCACCCTTC; GTGGGTCACTGCGGGGCCGACTGTGGCTGGGACCGGGGCGAGGTCTGCAGGGTGTGCCAG; AACAAGCTGTTTGAAAGCATCCAGGAAGGCAAGTATGAGTTTCCTGACAAGGACTGGGCA; CACATCTCCAGTGAAGCCAAAGACCTCATCTCCAAGCTCCTGGTGCGAGATGCAAAGCAG; AGACTTAGCGCCGCCCAAGTTCTGCAGCACCCATGGGTGCAGGGGCAAGCTCCAGAAAAG; GGACTCCCCACGCCGCAAGTCCTCCAGAGGAACAGCAGCACAATGGACCTGACGCTCTTC; GCAGCTGAGGCCATCGCCCTTAACCGCCAGCTATCTCAGCACGAAGAGAACGAACTAGCA; GAGGAGCCAGAGGCACTAGCTGATGGCCTCTGCTCCATGAAGCTTTCCCCTCCCTGCAAG; TCACGCCTGGCCCGGAGACGGGCCCTGGCCCAGGCAGGCCGTGGTGAAGACAGGAGCCCG; CCCACAGCACTCTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for AB000409 unedited; CGTGCAGNATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCGCGTTC; TCGGAGGAGCGATCTGCAGGTTTCCATGTCAGAGGCCGATGGAGAACTGAAGATTGCCAC; CTACGCACAAAGGCCATTGAGACACTTCGTGTAGCTGGAAGACACCAACTTCCTGACAGG; AGCTTTATTTCATTTGGGATTTCAAGTTTACAGATGGTATCTTCTCAAAAGTTGGAAAAA; CCTATAGAGATGGGCAGTAGCGAACCCCTTCCCATCGCAGATGGTGACAGGAGGAGGAAG; AAGAAGCGGAGGGGCCGGGCCACTGACTCCTTGCCAGGAAAGTTTGAAGATATGTACAAG; CTGACCTCTGAATTGCTTGGAGAGGGAGCCTATGCCAAAGTTCAAGGTGCCGTGAGCCTA; CAGAATGGCAAAGAGTATGCCGTCAAAATCATCGAGAAACAAGCAGGGCACAGTCGGAGT; AGGGTGTTTCGAGAGGTGGAGACGCTGTATCAGTGTCAGGGAAACAAGAACATTTTGGAG; CTGATTGAGTTCTTTGAAGATGACACAAGGTTTTACTTGGTCTTTGAGAAATTGCAAGGA; GGTTCCATCTTAGCCCACATCCAGAAGCAAAAGCACTTCAATGAGCGAGAAGCCAGCCGA; GTGGTGCGGGACGTTGCTGCTGCCCTTGACTTCCTGCATACCAAAGGCATTGCTCATCGT; GATCTGAAACCAGAAAATATATTGTGTGAATCTNCAGAAAAGGTGTCTCCAGTGAAAATC; TGTGACTTTGACTTGNGCAGTGGGATGAAACTGAACAACTCCTGTACCCCCATAACCACA; CCAGAGCTGACCACCCCATGTGGCTCTGCAGATACATGGCNNCCTGAGTAGTGGAGGGTC; TCACGGACCANGCCACATT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for AB000409 unedited; GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAGAATTTCAGTTTTATTAAAAT; AAGCACAATGTATAAAAGCACCGTGGTGTTGTATAAACGTCTGCCTGACAAATGCAAATC; TATTTTCTTTATGTAACTCAATAGTTCCACTTATCTGAATGGCTGTACCTTCATGCACAC; GGGCAGCAGGCACTGCATTGCTCAAACAGGGAAGTGAGGCTTCACCCTAGTGTGGCTTCA; CCTTAGGCACAGAGCACTATCTAACTGCCCAGATCTGTGCCACCCACACAAGACCTGGGG; ACACAGCAGCAGACACCGATGCTGTCTCTAAGTTCATCACAGGAACAGCTTAAAATCAGA; ATAATAGGGAACTGCTGATTAAAACCCAAGTGCATGGACATTCTAGAAAGAAGAGCAAGC; CGTTCCACTCTCTGGGAAGTTCATGGGCTTGCAGTGGGAGTTTAAGGTATCATCTTTGCT; GGAACAGCATGGTTAAAAACACCAAACTTTTTTTTTAAAAAAACGATACTACTGACTTTA; GCAGCAATCACAAACAAAGGGGATGATAAACGAGGGATACATTTCAGTCACAGCAAAGAA; AGATTATGTGCTTTTCCGTGCTGCAAACCGGAAGACTGATTTGATTCCCTGAATGACGAA; CGCTCCCTGCCCCTTCCTGATTGAGGGGATGGCTCTGCTCACCCCAAGGAGAATACGGCC; AAAAAGACATCCCGAGGACAAAAGAAGGTGGTAAGACCCCCATAGGAGGGGACCCAAAAT; AGAGGGACGAGCCCCTGTCGGCAACCTTTGAAACTGAGGCTTTTGCCTTTCCCTGAAGGT; GGAACCCCCAACCCCCCAACTACCACGGTGAAGAGAAGCTTGTATCCACAATCTTGGAGG; GGGTGAACAGGGCGAGACCCACAGAAAAAAATTTCTTCGTCCACCGCAATCATCCCAAGA; GAATAGGATGGCTTTCCCTTTCAAAAAAACCACTTCGAGAAAG;
• Restriction Sites: NotI-NotI
• ACCN: AB000409
• Insert Size: 2740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: AB000409.1, BAA19885.1
• RefSeq Size: 2617 bp
• RefSeq ORF: 1275 bp
• Locus ID: 8569
• Cytogenetics: 1p33
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Insulin signaling pathway, MAPK signaling pathway
• Summary: This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012]