LYRIC (MTDH) (NM_178812) Human Untagged Clone

SKU SC107267

MTDH (untagged)-Human metadherin (MTDH)

Product Data

• Type: Human Untagged Clone
• Target Symbol: LYRIC
• Synonyms: 3D3; AEG-1; AEG1; LYRIC; LYRIC/3D3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_178812, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCTGCACGGAGCTGGCAGGACGAGCTGGCCCAGCAGGCCGAGGAGGGCTCGGCCCGGCTGCGGGAAA; TGCTCTCGGTCGGCCTAGGCTTTCTGCGCACCGAGCTGGGCCTCGACCTGGGGCTGGAGCCGAAACGGTA; CCCCGGCTGGGTGATCCTGGTGGGCACTGGCGCGCTCGGGCTGCTGCTGCTGTTTCTGCTGGGCTACGGC; TGGGCCGCGGCTTGCGCCGGCGCCCGCAAAAAGCGGAGGAGCCCGCCCCGCAAGCGGGAGGAGGCGGCGG; CCGTGCCGGCCGCGGCCCCCGACGACCTGGCCTTGCTGAAGAATCTCCGGAGCGAGGAACAGAAGAAGAA; GAACCGGAAGAAACTGTCCGAGAAGCCCAAACCAAATGGGCGGACTGTTGAAGTGGCTGAGGGTGAAGCT; GTTCGAACACCTCAAAGTGTAACAGCAAAGCAGCCACCAGAGATTGACAAGAAAAATGAAAAGTCAAAGA; AAAATAAGAAGAAATCAAAGTCAGATGCTAAAGCAGTGCAAAACAGTTCACGCCATGATGGAAAGGAAGT; TGATGAAGGAGCCTGGGAAACTAAAATTAGTCACAGAGAGAAACGACAGCAGCGTAAACGTGATAAGGTG; CTGACTGATTCTGGTTCATTGGATTCAACTATCCCTGGGATAGAAAATACCATCACAGTTACCACCGAGC; AACTTACAACCGCATCATTTCCTGTTGGTTCCAAGAAGAATAAAGGTGATTCTCATCTAAATGTTCAAGT; TAGCAACTTTAAATCTGGAAAAGGAGATTCTACACTTCAGGTTTCTTCAGGATTGAATGAAAACCTCACT; GTCAATGGAGGAGGCTGGAATGAAAAGTCTGTAAAACTCTCCTCACAGATCAGTGCAGGTGAGGAGAAGT; GGAACTCCGTTTCACCTGCTTCTGCAGGAAAGAGGAAAACTGAGCCATCTGCCTGGAGTCAAGACACTGG; AGATGCTAATACAAATGGAAAAGACTGGGGAAGGAGTTGGAGTGACCGTTCAATATTTTCTGGCATTGGG; TCTACTGCTGAGCCAGTTTCTCAGTCTACCACTTCTGATTATCAGTGGGATGTTAGCCGTAATCAACCCT; ATATCGATGATGAATGGTCTGGGTTAAATGGTCTGTCTTCTGCTGATCCCAACTCTGATTGGAATGCACC; AGCAGAAGAGTGGGGCAATTGGGTAGACGAAGAAAGAGCTTCACTTCTAAAGTCCCAGGAACCAATTCCT; GATGATCAAAAGGTCTCAGATGATGATAAAGAAAAGGGAGAGGGAGCTCTTCCAACTGGGAAATCCAAAA; AGAAAAAAAAGAAAAAGAAGAAGCAAGGTGAAGATAACTCTACTGCACAGGACACAGAAGAATTAGAAAA; AGAGATTAGAGAAGACCTTCCAGTGAATACCTCTAAAACCCGTCCAAAACAGGAAAAAGCTTTTTCCTTG; AAGACCATAAGCACTAGTGATCCAGCCGAAGTACTCGTCAAAAATAGCCAGCCTATCAAGACTCTTCCAC; CTGCTACTTCTACCGAGCCATCTGTAATCTTATCAAAAAGTGATTCTGACAAGAGCTCTTCCCAAGTGCC; GCCAATACTACAAGAGACAGATAAATCCAAGTCAAATACCAAGCAAAATAGTGTGCCTCCTTCACAGACC; AAGTCTGAAACTAGCTGGGAATCTCCCAAACAAATAAAAAAGAAGAAAAAAGCCAGACGAGAAACGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_178812 unedited; CACCAGGCGGTGGCAGCGGCCGATCCCCGGCTCCGGCGCGAGGGACGGCCGCGATGCGCT; CGGCCTGAGGTTACCCGGCCCGGCCCTTCCTCGCTTCCCTCGACTATTCCACTGCGTCTC; CGCGCCCCGGCGTCATCCTGCGAGTCCCTCTGACGGGAGGGAAGATGGCTGCACGGAGCT; GGCAGGACGAGCTGGCCCAGCAGGCCGAGGAGGGCTCGGCCCGGCTGCGGGAAATGCTCT; CGGTCGGCCTAGGCTTTCTGCGCACCGAGCTGGGCCTCGACCTGGGGCTGGAGCCGAAAC; GGTACCCCGGCTGGGTGATCCTGGTGGGCACTGGCGCGCTCGGGCTGCTGCTGCTGTTTC; TGCTGGGCTACGGCTGGGCCGCGGCTTGCGCCGGCGCCCGCAAAAAGCGGAGGAGCCCGC; CCCGCAAGCGGGAGGAGGCGGCGGCCGTGCCGGCCGCGGCCCCCGACGACCTGGCCTTGC; TGAAGAATCTCCGGAGCGAGGAACAGAAGAAGAAGAACCGGAAGAAACTGTCCGAGAAGC; CCAAACCAAATGGGCGGACTGTTGAAGTGGCTGAGGGTGAAGCTGTTCGAACACCTCANA; GTGTAACAGCAAAGCAGCCACCAGAGATTGANCAGAAAAATGAAAAGTCAAAGAAAAATA; AGAAGAAATCAAAGTCAGATGCTAAAGCAGTGCANAACAGTTCACGCCATGATGGAAAGG; AAGTTGATGAAGGAGCCTGNGAAACTAAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_178812
• Insert Size: 1850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_178812.2, NP_848927.1
• RefSeq Size: 3729 bp
• RefSeq ORF: 1749 bp
• Locus ID: 92140
• UniProt ID: Q86UE4
• Cytogenetics: 8q22.1
• Protein Families: Transmembrane
• Summary: Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.[UniProtKB/Swiss-Prot Function]