PLD6 (NM_178836) Human Untagged Clone

SKU SC107263

PLD6 (untagged)-Human phospholipase D family, member 6 (PLD6)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PLD6
• Synonyms: ZUC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_178836, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGGACGGTTGAGTTGGCAGGTGGCGGCCGCGGCGGCTGTGGGCCTGGCTCTGACTCTGGAGGCGCTGC; CTTGGGTGCTGCGCTGGCTGCGGTCCAGGCGGCGGCGGCCGCGGCGCGAGGCGCTGTTCTTCCCGTCTCA; GGTGACCTGTACCGAGGCCCTGCTGCGGGCTCCGGGCGCGGAGCTGGCCGAGCTCCCCGAGGGCTGCCCG; TGCGGCCTGCCCCACGGCGAGAGCGCGCTAAGCCGCCTGCTGCGTGCCCTGCTGGCCGCCCGCGCCAGCC; TGGATCTCTGCCTGTTCGCCTTCTCCAGCCCGCAGCTGGGCCGCGCCGTGCAGTTGCTGCACCAGCGTGG; GGTGCGAGTGCGGGTCGTCACCGACTGCGACTACATGGCCCTCAACGGCTCGCAAATCGGTCTGCTGCGC; AAGGCAGGGATCCAGGTCCGGCACGATCAAGACCCAGGCTACATGCATCACAAGTTTGCCATCGTGGACA; AGAGGGTGCTCATCACTGGCTCGCTCAACTGGACCACGCAAGCCATCCAGAACAACAGGGAGAATGTTCT; CATCACGGAGGACGACGAGTACGTGCGGCTTTTTCTGGAAGAATTTGAGCGCATCTGGGAACAGTTTAAC; CCTACAAAGTATACCTTTTTCCCACCAAAGAAAAGTCACGGAAGCTGTGCCCCACCTGTCTCCAGAGCTG; GAGGGAGATTGCTTTCATGGCACAGAACTTGCGGCACCTCCAGCGAAAGCCAAACCTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_178836 unedited; ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGTCCCGGATT; AGCGGCGGCATGGGACGGTTGAGTTGGCAGGTGGCGGCCGCGGCGGCTGTGGGCCTGGCT; CTGACTCTGGAGGCGCTGCCTTGGGTGCTGCGCTGGCTGCGGTCCAGGCGGCGGCGGCCG; CGGCGCGAGGCGCTGTTCTTCCCGTCTCAGGTGACCTGTACCGAGGCCCTGCTGCGGGCT; CCGGGCGCGGAGCTGGCCGAGCTCCCCGAGGGCTGCCCGTGCGGCCTGCCCCACGGCGAG; AGCGCGCTAAGCCGCCTGCTGCGTGCCCTGCTGGCCGCCCGCGCCAGCCTGGATCTCTGC; CTGTTCGCCTTCTCCAGCCCGCAGCTGGGCCGCGCCGTGCAGTTGCTGCACCAGCGTGGG; GTGCGAGTGCGGGTCGTCACCGACTGCGACTACATGGCCCTCAACGGCTCGCAAATCGGT; CTGCTGCGCAAGGCAGGGATCCAGGTCCGGCACGATCAAGACCCAGGCTACATGCATCAC; AAGTTTGCCATCGTGGACAAGAGGGTGCTCATCACTGGCTCGCTCAACTGGACCACGCAA; GCCATCCAGAACAACAGGGAGAATGTTCTCATCACGGAGGACGACGAGTACGTGCGGCTT; TTTCTGGAAGAATTTGAGCGCATCTGGGAACAGTTTAACCCTACAAAGTATACCTTTTTC; CCACCAAAGAAAAGTCACGGAAGCTGTGCCCCACCTGTCTCCAGAGCTGGAGGGAGATTG; CTNTCATGGCACAGAAACTGCGGCACCTCCAGCGAAAGCCANACCTAACCAAGAATGGNN; GGCTGAGCCCTNCCCTGCGGNCTGCTGN;
• Restriction Sites: NotI-NotI
• ACCN: NM_178836
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_178836.2, NP_849158.1
• RefSeq Size: 2577 bp
• RefSeq ORF: 759 bp
• Locus ID: 201164
• UniProt ID: Q8N2A8
• Cytogenetics: 17p11.2
• Protein Families: Transmembrane
• Summary: Endonuclease that plays a critical role in PIWI-interacting RNA (piRNA) biogenesis during spermatogenesis. piRNAs provide essential protection against the activity of mobile genetic elements (By similarity). piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface (By similarity). Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low (PubMed:21397848). In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol (PubMed:21397848). Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity. Produces 5' phosphate and 3' hydroxyl termini, suggesting it could directly participate in the processing of primary piRNA transcripts (By similarity). Also acts as a regulator of mitochondrial shape through facilitating mitochondrial fusion (PubMed:17028579, PubMed:26711011).[UniProtKB/Swiss-Prot Function]