TRIM56 (NM_030961) Human Untagged Clone

CAT#: SC107178

TRIM56 (untagged)-Human tripartite motif containing 56 (TRIM56)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRIM56
• Synonyms: RNF109
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107178 sequence for NM_030961 edited (data generated by NextGen Sequencing)
  ATGGTTTCCCACGGGTCCTCGCCCTCCCTCCTGGAGGCCCTGAGCAGCGACTTCCTGGCC
  TGTAAAATCTGCCTGGAGCAGCTGCGGGCACCCAAGACACTGCCCTGCCTGCATACCTAC
  TGCCAAGACTGCCTGGCACAGCTGGCGGATGGCGGCCGCGTCCGCTGCCCCGAGTGCCGC
  GAGACAGTGCCTGTGCCGCCCGAGGGTGTGGCCTCCTTCAAGACCAACTTCTTCGTCAAT
  GGGCTGCTGGACCTGGTGAAGGCCCGGGCCTGTGGAGACCTGCGTGCCGGGAAGCCAGCC
  TGTGCCCTGTGTCCCCTGGTGGGTGGCACCAGCACCGGGGGGCCGGCCACGGCCCGGTGC
  CTGGACTGTGCCGATGACTTGTGCCAGGCCTGTGCCGACGGGCACCGCTGCACCCGCCAG
  ACCCACACCCACCGCGTGGTGGACCTGGTGGGCTACAGGGCCGGGTGGTATGATGAGGAG
  GCCCGGGAGCGCCAAGCGGCCCAGTGTCCCCAGCACCCCGGGGAGGCACTGCGCTTCCTG
  TGCCAGCCCTGCTCACAGTTGCTGTGCAGAGAGTGCCGCCTAGACCCCCACCTGGACCAC
  CCCTGCCTGCCTCTGGCTGAAGCTGTGCGTGCCCGGAGGCCGGGCCTGGAGGGACTGCTG
  GCCGGTGTGGACAATAACCTGGTGGAGCTGGAGGCAGCGCGGAGGGTGGAGAAGGAGGCG
  CTAGCCCGGCTGCGGGAGCAGGCGGCCCGGGTGGGGACTCAGGTGGAGGAGGCGGCTGAG
  GGCGTCCTCCGGGCCCTGCTGGCCCAGAAGCAGGAGGTGCTGGGGCAGCTACGAGCCCAC
  GTGGAGGCTGCCGAAGAAGCTGCTCGGGAGAGGCTGGCGGAGCTTGAGGGCCGGGAGCAG
  GTGGCCAGGGCCGCAGCCGCCTTCGCCCGCCGGGTACTCAGCCTGGGGCGAGAGGCCGAG
  ATCCTCTCCCTGGAAGGGGCGATCGCACAGCGGCTCAGGCAGCTGCAGGGCTGCCCCTGG
  GCACCAGGCCCGGCCCCCTGCCTGTTCCCACAGCTGGAGCTCCATCCTGGGCTCCTGGAC
  AAGAACTGCCACCTTCTTCGGCTGTCCTTTGAGGAGCAGCAGCCCCAGAAGGATGGTGGG
  AAAGACGGAGCTGGTACCCAGGGAGGTGAGGAGAGCCAGAGCCGGAGGGAGGATGAGCCG
  AAGACTGAGAGACAGGGTGGAGTCCAGCCCCAGGCTGGAGATGGAGCCCAGACCCCAAAA
  GAGGAAAAAGCCCAGACAACCCGAGAAGAGGGAGCCCAGACCTTGGAGGAGGACAGGGCC
  CAGACACCCCACGAGGATGGAGGACCCCAGCCCCACAGGGGTGGCAGACCCAACAAGAAG
  AAAAAGTTCAAAGGCAGGCTCAAGTCAATTTCCCGGGAGCCCAGCCCAGCCCTGGGGCCG
  AATCTGGACGGCTCTGGCCTCCTCCCCAGACCCATCTTTTACTGCAGTTTCCCCACGCGG
  ATGCCTGGAGACAAGCGGTCCCCCCGGATCACCGGGCTCTGTCCCTTCGGTCCCCGGGAG
  ATCCTGGTGGCGGATGAGCAGAACCGGGCACTGAAACGCTTCTCCCTCAACGGCGACTAC
  AAGGGCACCGTGCCGGTCCCTGAGGGCTGCTCCCCTTGCAGCGTGGCCGCCCTGCAGAGC
  GCGGTGGCCTTCTCCGCTAGCGCACGGCTCTATCTCATCAACCCCAACGGCGAAGTGCAG
  TGGCGCAGGGCCCTGAGCCTCTCCCAGGCCAGCCACGCGGTGGCGGCACTGCCTAGCGGG
  GACCGCGTGGCTGTCAGCGTGGCGGGCCACGTGGAGGTGTACAATATGGAAGGCAGCCTG
  GCCACCCGGTTCATTCCTGGAGGCAAGGCCAGCCGGGGCCTGCGGGCGCTGGTGTTTCTG
  ACCACCAGCCCCCAGGGGCATTTCGTGGGGTCGGACTGGCAGCAGAATAGTGTGGTAATC
  TGTGATGGGCTGGGCCAGGTGGTTGGGGAGTACAAGGGGCCAGGCCTGCATGGCTGCCAG
  CCGGGCTCCGTGTCTGTGGATAAGAAGGGCTACATCTTTCTGACCCTTCGAGAAGTCAAC
  AAGGTGGTGATCCTGGACCCGAAGGGGTCCCTCCTTGGAGACTTCCTGACAGCCTACCAC
  GGCCTGGAAAAGCCCCGGGTTACCACCATGGTGGATGGCAGGTACCTGGTCGTGTCCCTC
  AGTAACGGGACCATCCACATCTTTCGGGTCCGTTCTCCGGACAGTTAA
  
  Clone variation with respect to NM_030961.1
  912 g=>c;1045 c=>t;1236 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030961 unedited
  TTTCCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC
  AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG
  AATTCGGCACGAGGCAAGATCCTACCCCAACTGGCAGGACCTAGGCATCTCTGCCCACTG
  GGATCCCCGTCTGTGTCCTTACTGCCTGCTCCTCGCACCCTGGCACCCTATACAGCAGGA
  CCCCAGTACCTGTGCACACAGGAGGGCTTCCACCATGTTTGCCACAAGGCCTGTGGACTG
  GGGACACTGAGGATTCGATTATTAGCTAAGGCCAGGGACTCCTGGTCTTGTCAGTTCTCA
  CAGGTCTGACCCAACAAGTAGCACTGACATTTTTACGTTTGCTGGATGTACACACGGAAG
  TGGAGGAGGAGGAGGAGAAGGAGGAGGGCAGCTCCTTAGCTCAAGAGCAAGTGGCCCAAG
  GCCTCAGAAGACTAGAAGGAAGTTCCTGGCCATTCAGCATGGTTTCCCACGGGTCCTCGC
  CCTCCCTCCTGGAGGCCCTGAGCAGCGACTTCCTGGCCTGTAAAATCTGCCTGGAGCAGC
  TGCGGGCACCCAAGACACTGCCCTGCCTGCATACCTACTGCCAAGACTGCCTGGCACAGC
  TGGCGGATGGCGGCCGCGTCCGCTGCCCCGAGTGCCGCGAGACAGTGCCTGTGCCGCCCG
  AGGGTGTGGCCTCCTTCAAGACCAACTTCTTCGTCAATGGGCTGCTGGACCTGGTGANAG
  CCCNGGCCTGTGGAGACCTGCGTGCNCGGAAGCCAGCCTGTGCCCTGTGTCCCCTGGTGG
  GTGGCACCAGCACCGGGGGGCCCGGCCACGCCCNGTGCCTGNACTGTGCCGATGACTTGT
  GCCANGCCTGTGCCGACNGGCACCGTTGCACCCGNCAGACCACACNCACCNGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030961 unedited
  AGTAAAACCTTTTTTTTTTTTTTTTCGAAAGGGGTCTGGGAAGCAGTGGTGCGATCATAG
  CTCACTGCAGACTCAAACTCCTCGGCTCAAGGGATCCTCCCACCTCAGCCTCCTGAGTAG
  CTACGTTGAAAGGCATATGCTACTACACGCAGCTAATTTTTAATCTTTTGTAGAGATGGG
  GTCTCGCTATGTTGCCCAGGCTGGTCTCAAACTCCTGGCCTCAAGCAATCCTCCTGCCAC
  AGACTCCCAAAGTGCCGGGATTATAGGCATGAGCCACTGCGCCTGGCAGAGCCTGGTTTT
  TTTTTAATACTTAATCTTAGCCAAAAGGCTGAGAAGCGATGGAGAATGTGGATTTTTGGA
  CGTGAATTCTGTTGGGTTTGGTTTTCCAGTAGCCTATTCTCTTCGCAGCCTTTGAACCTA
  ACACCTCTTTGGCCCCAGCTGCTGTCTGCACTTGCAGACCCTCCTGCAAGCTCACCCTGT
  ACTACCGCCGGGGGATGCCGTGATCAAGGAGCATCCTGCCTGTGGCTGGCAGAAGAGGCC
  ATTCACTGCCCATTCGAAATCTCAGGCCTCCTCTGACTCTGTAGGACCCTGTGCCAGTCC
  AAGGGGNGGCCGGGGTCTCAGCTCTGTTCCACAGTGCACACGCCTGACCCACCCTGGGCC
  CTTCTTGGCCCCAAGACTCTCCCTCGGCTACTCTATCAGGAATGGTTTTGAAGAATGCAA
  AGCAGGACAGGCTCACAAACCTCAGAAACCTAAACTATATAGCANCGGCGGGGGGTGGCA
  GCAGCCACCCACCAATAGCAGGAGGCAGTGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_030961
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030961.1, NP_112223.1
• RefSeq Size: 3547 bp
• RefSeq ORF: 2268 bp
• Locus ID: 81844
• UniProt ID: Q9BRZ2
• Cytogenetics: 7q22.1
• Gene Summary: E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity (PubMed:21289118). In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets TMEM173/STING to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta (By similarity). Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES. Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus (PubMed:22948160).[UniProtKB/Swiss-Prot Function]