TRIM56 (NM_030961) Human Untagged Clone

SKU SC107178

TRIM56 (untagged)-Human tripartite motif containing 56 (TRIM56)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRIM56
• Synonyms: RNF109
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107178 sequence for NM_030961 edited (data generated by NextGen Sequencing); ATGGTTTCCCACGGGTCCTCGCCCTCCCTCCTGGAGGCCCTGAGCAGCGACTTCCTGGCC; TGTAAAATCTGCCTGGAGCAGCTGCGGGCACCCAAGACACTGCCCTGCCTGCATACCTAC; TGCCAAGACTGCCTGGCACAGCTGGCGGATGGCGGCCGCGTCCGCTGCCCCGAGTGCCGC; GAGACAGTGCCTGTGCCGCCCGAGGGTGTGGCCTCCTTCAAGACCAACTTCTTCGTCAAT; GGGCTGCTGGACCTGGTGAAGGCCCGGGCCTGTGGAGACCTGCGTGCCGGGAAGCCAGCC; TGTGCCCTGTGTCCCCTGGTGGGTGGCACCAGCACCGGGGGGCCGGCCACGGCCCGGTGC; CTGGACTGTGCCGATGACTTGTGCCAGGCCTGTGCCGACGGGCACCGCTGCACCCGCCAG; ACCCACACCCACCGCGTGGTGGACCTGGTGGGCTACAGGGCCGGGTGGTATGATGAGGAG; GCCCGGGAGCGCCAAGCGGCCCAGTGTCCCCAGCACCCCGGGGAGGCACTGCGCTTCCTG; TGCCAGCCCTGCTCACAGTTGCTGTGCAGAGAGTGCCGCCTAGACCCCCACCTGGACCAC; CCCTGCCTGCCTCTGGCTGAAGCTGTGCGTGCCCGGAGGCCGGGCCTGGAGGGACTGCTG; GCCGGTGTGGACAATAACCTGGTGGAGCTGGAGGCAGCGCGGAGGGTGGAGAAGGAGGCG; CTAGCCCGGCTGCGGGAGCAGGCGGCCCGGGTGGGGACTCAGGTGGAGGAGGCGGCTGAG; GGCGTCCTCCGGGCCCTGCTGGCCCAGAAGCAGGAGGTGCTGGGGCAGCTACGAGCCCAC; GTGGAGGCTGCCGAAGAAGCTGCTCGGGAGAGGCTGGCGGAGCTTGAGGGCCGGGAGCAG; GTGGCCAGGGCCGCAGCCGCCTTCGCCCGCCGGGTACTCAGCCTGGGGCGAGAGGCCGAG; ATCCTCTCCCTGGAAGGGGCGATCGCACAGCGGCTCAGGCAGCTGCAGGGCTGCCCCTGG; GCACCAGGCCCGGCCCCCTGCCTGTTCCCACAGCTGGAGCTCCATCCTGGGCTCCTGGAC; AAGAACTGCCACCTTCTTCGGCTGTCCTTTGAGGAGCAGCAGCCCCAGAAGGATGGTGGG; AAAGACGGAGCTGGTACCCAGGGAGGTGAGGAGAGCCAGAGCCGGAGGGAGGATGAGCCG; AAGACTGAGAGACAGGGTGGAGTCCAGCCCCAGGCTGGAGATGGAGCCCAGACCCCAAAA; GAGGAAAAAGCCCAGACAACCCGAGAAGAGGGAGCCCAGACCTTGGAGGAGGACAGGGCC; CAGACACCCCACGAGGATGGAGGACCCCAGCCCCACAGGGGTGGCAGACCCAACAAGAAG; AAAAAGTTCAAAGGCAGGCTCAAGTCAATTTCCCGGGAGCCCAGCCCAGCCCTGGGGCCG; AATCTGGACGGCTCTGGCCTCCTCCCCAGACCCATCTTTTACTGCAGTTTCCCCACGCGG; ATGCCTGGAGACAAGCGGTCCCCCCGGATCACCGGGCTCTGTCCCTTCGGTCCCCGGGAG; ATCCTGGTGGCGGATGAGCAGAACCGGGCACTGAAACGCTTCTCCCTCAACGGCGACTAC; AAGGGCACCGTGCCGGTCCCTGAGGGCTGCTCCCCTTGCAGCGTGGCCGCCCTGCAGAGC; GCGGTGGCCTTCTCCGCTAGCGCACGGCTCTATCTCATCAACCCCAACGGCGAAGTGCAG; TGGCGCAGGGCCCTGAGCCTCTCCCAGGCCAGCCACGCGGTGGCGGCACTGCCTAGCGGG; GACCGCGTGGCTGTCAGCGTGGCGGGCCACGTGGAGGTGTACAATATGGAAGGCAGCCTG; GCCACCCGGTTCATTCCTGGAGGCAAGGCCAGCCGGGGCCTGCGGGCGCTGGTGTTTCTG; ACCACCAGCCCCCAGGGGCATTTCGTGGGGTCGGACTGGCAGCAGAATAGTGTGGTAATC; TGTGATGGGCTGGGCCAGGTGGTTGGGGAGTACAAGGGGCCAGGCCTGCATGGCTGCCAG; CCGGGCTCCGTGTCTGTGGATAAGAAGGGCTACATCTTTCTGACCCTTCGAGAAGTCAAC; AAGGTGGTGATCCTGGACCCGAAGGGGTCCCTCCTTGGAGACTTCCTGACAGCCTACCAC; GGCCTGGAAAAGCCCCGGGTTACCACCATGGTGGATGGCAGGTACCTGGTCGTGTCCCTC; AGTAACGGGACCATCCACATCTTTCGGGTCCGTTCTCCGGACAGTTAA; ; Clone variation with respect to NM_030961.1; 912 g=>c;1045 c=>t;1236 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030961 unedited; TTTCCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC; AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG; AATTCGGCACGAGGCAAGATCCTACCCCAACTGGCAGGACCTAGGCATCTCTGCCCACTG; GGATCCCCGTCTGTGTCCTTACTGCCTGCTCCTCGCACCCTGGCACCCTATACAGCAGGA; CCCCAGTACCTGTGCACACAGGAGGGCTTCCACCATGTTTGCCACAAGGCCTGTGGACTG; GGGACACTGAGGATTCGATTATTAGCTAAGGCCAGGGACTCCTGGTCTTGTCAGTTCTCA; CAGGTCTGACCCAACAAGTAGCACTGACATTTTTACGTTTGCTGGATGTACACACGGAAG; TGGAGGAGGAGGAGGAGAAGGAGGAGGGCAGCTCCTTAGCTCAAGAGCAAGTGGCCCAAG; GCCTCAGAAGACTAGAAGGAAGTTCCTGGCCATTCAGCATGGTTTCCCACGGGTCCTCGC; CCTCCCTCCTGGAGGCCCTGAGCAGCGACTTCCTGGCCTGTAAAATCTGCCTGGAGCAGC; TGCGGGCACCCAAGACACTGCCCTGCCTGCATACCTACTGCCAAGACTGCCTGGCACAGC; TGGCGGATGGCGGCCGCGTCCGCTGCCCCGAGTGCCGCGAGACAGTGCCTGTGCCGCCCG; AGGGTGTGGCCTCCTTCAAGACCAACTTCTTCGTCAATGGGCTGCTGGACCTGGTGANAG; CCCNGGCCTGTGGAGACCTGCGTGCNCGGAAGCCAGCCTGTGCCCTGTGTCCCCTGGTGG; GTGGCACCAGCACCGGGGGGCCCGGCCACGCCCNGTGCCTGNACTGTGCCGATGACTTGT; GCCANGCCTGTGCCGACNGGCACCGTTGCACCCGNCAGACCACACNCACCNGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030961 unedited; AGTAAAACCTTTTTTTTTTTTTTTTCGAAAGGGGTCTGGGAAGCAGTGGTGCGATCATAG; CTCACTGCAGACTCAAACTCCTCGGCTCAAGGGATCCTCCCACCTCAGCCTCCTGAGTAG; CTACGTTGAAAGGCATATGCTACTACACGCAGCTAATTTTTAATCTTTTGTAGAGATGGG; GTCTCGCTATGTTGCCCAGGCTGGTCTCAAACTCCTGGCCTCAAGCAATCCTCCTGCCAC; AGACTCCCAAAGTGCCGGGATTATAGGCATGAGCCACTGCGCCTGGCAGAGCCTGGTTTT; TTTTTAATACTTAATCTTAGCCAAAAGGCTGAGAAGCGATGGAGAATGTGGATTTTTGGA; CGTGAATTCTGTTGGGTTTGGTTTTCCAGTAGCCTATTCTCTTCGCAGCCTTTGAACCTA; ACACCTCTTTGGCCCCAGCTGCTGTCTGCACTTGCAGACCCTCCTGCAAGCTCACCCTGT; ACTACCGCCGGGGGATGCCGTGATCAAGGAGCATCCTGCCTGTGGCTGGCAGAAGAGGCC; ATTCACTGCCCATTCGAAATCTCAGGCCTCCTCTGACTCTGTAGGACCCTGTGCCAGTCC; AAGGGGNGGCCGGGGTCTCAGCTCTGTTCCACAGTGCACACGCCTGACCCACCCTGGGCC; CTTCTTGGCCCCAAGACTCTCCCTCGGCTACTCTATCAGGAATGGTTTTGAAGAATGCAA; AGCAGGACAGGCTCACAAACCTCAGAAACCTAAACTATATAGCANCGGCGGGGGGTGGCA; GCAGCCACCCACCAATAGCAGGAGGCAGTGAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_030961
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030961.1, NP_112223.1
• RefSeq Size: 3547 bp
• RefSeq ORF: 2268 bp
• Locus ID: 81844
• UniProt ID: Q9BRZ2
• Cytogenetics: 7q22.1
• Summary: E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity (PubMed:21289118). In response to pathogen- and host-derived double-stranded DNA (dsDNA), targets TMEM173/STING to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta (By similarity). Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES. Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus (PubMed:22948160).[UniProtKB/Swiss-Prot Function]