PPP2R4 (PTPA) (NM_178000) Human Untagged Clone

SKU SC107146

PPP2R4 (untagged)-Human protein phosphatase 2A activator, regulatory subunit 4 (PPP2R4), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: PPP2R4
• Synonyms: PP2A; PPP2R4; PR53
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_178000, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGGAGGCCCCTCCAGCCACTCAGAACTTCA; TCATTCCAAAAAAGGAGATCCACACAGTTCCAGACATGGGCAAATGGAAGCGTTCTCAGGCATACGCTGA; CTACATCGGATTCATCCTTACCCTCAACGAAGGTGTGAAGGGGAAGAAGCTGACCTTCGAGTACAGAGTC; TCCGAGGCCATTGAGAAACTAGTCGCTCTTCTCAACACGCTGGACAGGTGGATTGATGAGACTCCTCCAG; TGGACCAGCCCTCTCGGTTTGGGAATAAGGCATACAGGACCTGGTATGCCAAACTTGATGAGGAAGCAGA; AAACTTGGTGGCCACAGTGGTCCCTACCCATCTGGCAGCTGCTGTGCCTGAGGTGGCTGTTTACCTAAAG; GAGTCAGTGGGGAACTCCACGCGCATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCTGCTTTCCTCT; GCTGTCTCTGCAAGATTGGGGTGCTCCGGGTGGATGACCAAATAGCTATTGTCTTCAAGGTGTTCAATCG; GTACCTTGAGGTTATGCGGAAACTCCAGAAAACATACAGGATGGAGCCAGCCGGCAGCCAGGGAGTGTGG; GGTCTGGATGACTTCCAGTTTCTGCCCTTCATCTGGGGCAGTTCGCAGCTGATAGACCACCCATACCTGG; AGCCCAGACACTTTGTGGATGAGAAGGCCGTGAATGAGAACCACAAGGACTACATGTTCCTGGAGTGTAT; CCTGTTTATTACCGAGATGAAGACTGGCCCATTTGCAGAGCACTCCAACCAGCTGTGGAACATCAGCGCC; GTCCCTTCCTGGTCCAAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTGGAGAAGTTCC; CTGTGATCCAGCACTTCAAGTTCGGGAGCCTGCTGCCCATCCATCCTGTCACGTCGGGCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_178000 unedited; TCCCGCCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGA; GCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAAT; TCGGCACGAGGCCGGGTCCGCGCGTCCGCCGCGCGCCGGCCGTTAATAGGCTTGCTCCCT; GAGCGCCCCGCACCGACATGGCGGCCGTCTTCGCTGTGGTGACTTTAACTCTCGGTTTTC; GGTTATAGCCGGCCGGCGCTCACTTGTCTTCAGGAAGCTCGGAGCCTTTGGTGGAGCCGG; GGAGAGGAAGGGTGGGTGCAAGAGTGAAAGGCGAGAGGGGACTGCAAGCATCCGGGTCGG; CTCCTGGCCGGAGCAAGATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGG; AGGCCCCTCCAGCCACTCAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAG; ACATGGGCAAATGGAAGCGTTCTCAGGCATACGCTGACTACATCGGATTCATCCTTACCC; TCAACGAAGGTGTGAAGGGGAAGAAGCTGACCTTCGAGTACAGAGTCTCCGAGGCCATTG; AGAAACTAGTCGCTCTTCTCAACACGCTGGACAGGTGGATTGATGAGACTCCTCCAGTGG; ACCAGCCCTCTCGGTTTGGGAATAAGGCATACAGGACCTGNTATGCCAAACTTGATGAGG; AAGCAGAACACTTGGTGGCCACAGTGGTCCCTACCCATCTGGCAGCTGCTGTGCCTGAGG; TGGCTGTTTACCTAAAGGAGTCAGTGGGGAAACTCACGCGCATTGACTACGGCACAGGGC; ATGAGGCAGCCTTCGCTGCTTTCCTCTGCTGTCTCTGCAGAANTGGNGTGCTNCNGNNTG; GATGACCANNATAGCTATGTCTTTCACGGTGTTCAATCGGTACCTTTGAGGTTATGCNGN; AACTNCACGAAACATACAGGATGGAGCCAGNCGCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_178000 unedited; TCCAGTGGGGACTCATTGGCGATGGTCAACCTTCCCATGNCCAGNGAGAGCACTGGGGNA; GGGGTACACAGGGCATGCCACCCGGGTTCTGTATCAGNAAACAGCTATGACCGCGGCCGC; AATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGACAGGTGCAAAACAGAGGCTTTAATCT; GAACAGCAATGAGGGTGCAAGGGGCTGGGAGCCCATAGGGGGATGGGGGAGCCAGGAAGG; GGTGTCTGGCCCTGGGAGAGAGGTGGACGCTGGGCCTCTTCTGAGAAACTGTCACTGACC; TTTGGGACTCCTGACCCAGCCTCTGCCCAGACCATCACCGGATCCCAGGCTGAGCCCCTG; TGCTGTGCTTCTCAGTGCTCCCCCTTCCTGAAGGGTGTGTGGACCCAGACACAGGAGGGG; GCAGTGGAGAAGGGCAGGCCTGGGGGACGAGCCTGTAAGAGCCAGGCCCAGGAACAGCCC; TAGGAGAAAACCTCGGCGGTGGCCCTCAATGGGCCTGCCCAGCCCCCACTGGGCCCATTC; TGAGTGCCAAAGCCTGGGCTTGGGAGGCTGTCACTTACCTCACCCTATAAAAGCAATGGA; CAGTTCAGGGAGGTGGGAGGACAAGGTGTGGGACTGCCACCTTCTGGGCCCACCCATCAC; CCAGGGGAGCAGANAAGCAGGGCCCAGGGGCCCAGGGTGAGGCTCACAGCAGCGCAAAGG; TAGCAGAAGTGGCTGGGCCTAAGACTGCCAAGAGGAATCCAGGCTCTCCAGATACAATGG; GCCCCAAGCCCCTCCCTGAGTTTCTGCCATCCCCTCCCTGCCAGGGAAAGTTCTGGAAAA; GACATGCTCACCCCATCATCTGGGCCCCTCCCAGGACCCCAGCACCTACCCAAAGGGCAG; CTTCCAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_178000
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_178000.1, NP_821067.1
• RefSeq Size: 2736 bp
• RefSeq ORF: 972 bp
• Locus ID: 5524
• UniProt ID: Q15257
• Cytogenetics: 9q34.11
• Protein Families: Druggable Genome, Phosphatase
• Summary: Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks one of the coding exons compared to transcript variant 1. It maintains the reading frame and encodes a protein (isoform b), also known as isoform alpha, that is missing an internal segment of the protein compared to isoform a. Variants 2 and 3 encode the same isoform.