CRY2 (NM_021117) Human Untagged Clone

CAT#: SC107056

CRY2 (untagged)-Human cryptochrome 2 (photolyase-like) (CRY2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRY2
• Synonyms: HCRY2; PHLL2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021117, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCGGGGTCCACGTCGCCTACCGGGGCGGAGCGGGGGTGGCTGGAGCAGTCTGGACAGTCATGGCGG
  CGACTGTGGCGACGGCGGCAGCTGTGGCCCCGGCGCCAGCGCCCGGCACGGACAGCGCCTCTTCGGTGCA
  CTGGTTCCGCAAAGGGCTGCGACTCCACGACAACCCGGCGTTGCTGGCGGCCGTGCGCGGGGCGCGCTGC
  GTGCGCTGCGTTTACATTCTCGACCCGTGGTTCGCGGCCTCCTCCTCAGTCGGGATCAACCGATGGAGGT
  TCCTACTTCAGTCTCTGGAAGATTTGGACACAAGTTTAAGGAAACTGAACTCCCGCCTGTTTGTAGTCCG
  GGGACAGCCAGCCGACGTGTTCCCAAGGCTGTTCAAGGAATGGGGAGTGACCCGCTTGACCTTTGAATAT
  GACTCTGAACCCTTTGGGAAAGAACGGGATGCAGCCATCATGAAGATGGCCAAGGAGGCTGGTGTGGAAG
  TAGTGACGGAGAATTCTCATACCCTCTATGACCTGGACAGGATCATTGAGCTGAATGGGCAGAAGCCACC
  CCTTACATACAAGCGCTTTCAGGCCATCATCAGCCGCATGGAGCTGCCCAAGAAGCCAGTGGGCTTGGTG
  ACCAGCCAGCAGATGGAGAGCTGCAGGGCCGAGATCCAGGAGAACCACGACGAGACCTACGGCGTGCCCT
  CCCTGGAGGAGCTGGGGTTCCCCACTGAAGGACTTGGTCCAGCTGTCTGGCAGGGAGGAGAGACAGAAGC
  TCTGGCCCGCCTGGATAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCCCGAATGAAC
  GCCAACTCCCTCCTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGTTGTCTCTCCTGCCGCC
  TCTTCTACTACCGCCTGTGGGACCTGTATAAAAAGGTGAAGCGGAACAGCACACCTCCCCTCTCCCTATT
  TGGGCAACTCCTATGGCGAGAGTTCTTCTACACGGCAGCTACCAACAACCCCAGGTTTGACCGCATGGAG
  GGGAACCCCATCTGCATCCAGATCCCCTGGGACCGCAATCCTGAGGCCCTGGCCAAGTGGGCTGAGGGCA
  AGACAGGCTTCCCTTGGATTGATGCCATCATGACCCAACTGAGGCAGGAGGGCTGGATCCACCACCTGGC
  CCGGCATGCCGTGGCCTGCTTCCTGACCCGCGGGGACCTCTGGGTCAGCTGGGAGAGCGGGGTCCGGGTA
  TTTGATGAGCTGCTCCTGGATGCAGATTTCAGCGTGAACGCAGGCAGCTGGATGTGGCTGTCCTGCAGTG
  CTTTCTTCCAGCAGTTCTTCCACTGCTACTGCCCTGTGGGCTTTGGCCGTCGCACGGACCCCAGTGGGGA
  CTACATCAGGCGATACCTGCCCAAATTGAAAGCGTTCCCCTCTCGATACATCTATGAGCCCTGGAATGCC
  CCAGAGTCAATTCAGAAGGCAGCCAAGTGCATCATTGGTGTGGACTACCCACGGCCCATCGTCAACCATG
  CCGAGACCAGCCGGCTTAACATTGAACGAATGAAGCAGATTTACCAGCAGCTTTCGCGCTACCGGGGACT
  CTGTCTACTGGCATCTGTCCCTTCCTGTGTGGAAGACCTCAGTCACCCTGTGGCAGAGCCCAGCTCGAGC
  CAGGCTGGCAGCATGAGCAGTGCAGGCCCAAGACCACTACCCAGTGGCCCAGCATCCCCCAAACGCAAGC
  TGGAAGCAGCCGAGGAACCACCTGGTGAAGAACTCAGCAAACGGGCCCGGGTGGCAGAGTTGCCAACCCC
  AGAGCTGCCGAGCAAGGATGCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021117 unedited
  TGTATACGTATCACTATAGGCGGCCGCGAATTCGCACGAGGCTGGAGCAGTCTGGACAGT
  CATGGCGGCGACTGTGGCGACGGCGGCAGCTGTGGCCCCGGCGCCAGCGCCCGGCACGGA
  CAGCGCCTCTTCGGTGCACTGGTTCCGCAAAGGGCTGCGACTCCACGACAACCCGGCGTT
  GCTGGCGGCCGTGCGCGGGGCGCGCTGCGTGCGCTGCGTTTACATTCTCGACCCGTGGTT
  CGCGGCCTCCTCCTCAGTCGGGATCAACCGATGGAGGTTCCTACTTCAGTCTCTGGAAGA
  TTTGGACACAAGTTTAAGGAAACTGAACTCCCGCCTGTTTGTAGTCCGGGGACAGCCAGC
  CGACGTGTTCCCAAGGCTGTTCAAGGAATGGGGAGTGACCCGCTTGACCTTTGAATATGA
  CTCTGAACCCTTTGGGAAAGAACGGGATGCAGCCATCATGAAGATGGCCAAGGAGGCTGG
  TGTGGAAGTAGTGACGGAGAATTCTCATACCCTCTATGACCTGGACAGGATCATTGAGCT
  GAATGGGCAGAAGCCACCCCTTACATACAAGCGCTTTCAGGCCATCATCAGCCGCATGGA
  GCTGCCCAAGAAGCCAGTGGGCTTGGTGACCAGCCAGCAGATGGAGAGCTGCAGGGCCGA
  GATCCAGGAGAACCACGACGAGACCTACGGCGTGCCCTCCCTGGAGGAGCTGGGGTTCCC
  CACTGNAAGACTTGGTCCAGCTGTCTGGCAGGGAGGAGAGACAGAAGCTCTGGCCCCGCT
  GGATAAGCACTTGGAACGGAAAGCCCTGGTTGCCACTATGAGAGACCCGAATGAACGCAA
  CTCCTNCTGGCCAGCCCACAGGCTNAGNCCCTACCTGCGCTTGGTGTCTCTCTGGCGCTC
  TCTATAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021117
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021117.1, NP_066940.1
• RefSeq Size: 4204 bp
• RefSeq ORF: 1782 bp
• Locus ID: 1408
• UniProt ID: Q49AN0
• Cytogenetics: 11p11.2
• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) uses an alternate first exon which contains the predicted translation start site; the resulting protein has a distinct N-terminus. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation.