CKII alpha (CSNK2A1) (NM_177559) Human Untagged Clone

SKU SC107027

CSNK2A1 (untagged)-Human casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CKII alpha
• Synonyms: CK2A1; Cka1; Cka2; CKII; OCNDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_177559 edited; ATGTCGGGACCCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCT; CGAGAATACTGGGATTACGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCAG; CTGGTTCGAAAATTAGGCCGAGGTAAATACAGTGAAGTATTTGAAGCCATCAACATCACA; AATAATGAAAAAGTTGTTGTTAAAATTCTCAAGCCAGTAAAAAAGAAGAAAATTAAGCGT; GAAATAAAGATTTTGGAGAATTTGAGAGGAGGTCCCAACATCATCACACTGGCAGACATT; GTAAAAGACCCTGTGTCACGAACCCCCGCCTTGGTTTTTGAACACGTAAACAACACAGAC; TTCAAGCAATTGTACCAGACGTTAACAGACTATGATATTCGATTTTACATGTATGAGATT; CTGAAGGCCCTGGATTATTGTCACAGCATGGGAATTATGCACAGAGATGTCAAGCCCCAT; AATGTCATGATTGATCATGAGCACAGAAAGCTACGACTAATAGACTGGGGTTTGGCTGAG; TTTTATCATCCTGGCCAAGAATATAATGTCCGAGTTGCTTCCCGATACTTCAAAGGTCCT; GAGCTACTTGTAGACTATCAGATGTACGATTATAGTTTGGATATGTGGAGTTTGGGTTGT; ATGCTGGCAAGTATGATCTTTCGGAAGGAGCCATTTTTCCATGGACATGACAATTATGAT; CAGTTGGTGAGGATAGCCAAGGTTCTGGGGACAGAAGATTTATATGACTATATTGACAAA; TACAACATTGAATTAGATCCACGTTTCAATGATATCTTGGGCAGACACTCTCGAAAGCGA; TGGGAACGCTTTGTCCACAGTGAAAATCAGCACCTTGTCAGCCCTGAGGCCTTGGATTTC; CTGGACAAACTGCTGCGATATGACCACCAGTCACGGCTTACTGCAAGAGAGGCAATGGAG; CACCCCTATTTCTACACTGTTGTGAAGGACCAGGCTCGAATGGGTTCATCTAGCATGCCA; GGGGGCAGTACGCCCGTCAGCAGCGCCAATATGATGTCAGGGATTTCTTCAGTGCCAACC; CCTTCACCCCTTGGACCTCTGGCAGGCTCACCAGTGATTGCTGCTGCCAACCCCCTTGGG; ATGCCTGTTCCAGCTGCCGCTGGCGCTCAGCAGTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_177559 unedited; TGTAATACGACTCACTATAGGGCGGCCGCGCAATTCGCACGAGGGTCTCCCCCCTCCATC; GCCGCCATATTGTCTGTGTGAGCAGAGGGGAGAGCGGCCGCCGCCGCTGCCGCTTCCACC; ACAGCTCTATCAAGGCTTGTCAAGCAGTGTGCTCATCACATCGTAAATCATGCAGCGTGG; AACCTCATAAAATCTCCAACAAACATCATTCACCCATACTGACTAGTTTCACATCTCTTT; GTTTGAAGAAAACAGGTCTGAAACAAGGTCTTACCCCCAGCTGCTTCTGAACACAGTGAC; TGCCAGATCTCCAAACATCAAGTCCAGCTTTGTCCGCCAACCTGTCTGACATGTCGGGAC; CCGTGCCAAGCAGGGCCAGAGTTTACACAGATGTTAATACACACAGACCTCGAGAATACC; TGGGATTACGAGTCACATGTGGTGGAATGGGGAAATCAAGATGACTACCAGCTGGTTCGA; AAATTACGCCGAGGTAAATACAGTGAAGTATTTGAAGCCATCAACATCACAAATAATGAA; AAAGTTGTTGCTAAAATTCTCAAGCCAGCAAAAAAGAACAAATTAAGCGTGAAATAAAGA; TTTTGGAGAATTTGAGAGGAGGTCCCACCATCATCACACTGGCAGACATGTAAAAGACCC; TGTGTCACGAACCCCCGCCTTGGTTTTGAACACCAAACACACAGACTTAAGCAATTGTAC; CAGACGTCTACAGACTATGAATATCCGATCTTACATGTATGACATTCCGAAAGCCCTTGA; ATATTGTCCCAGCATGGGAATTATGCACAGAGATGCCAGCCCCATAAAGTCATGATTGAC; CCGGACCCCAAAAGCCTCACCAATAGAACCGCGTTTGGCTGAGTTTTATCATCCGGCCAA; CAATAAAGCCCGCAGTGCCTCCGAACCTTAAGGCCCGGGCTTC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_177559 unedited; CTGTTACCGCGCCCGCATTCTANATCGAGTTTTTTCTTTTTTTTTTTCACTATGAAGGAC; AAAGTATTATATATATACACACAGCAAGGGGTGGCGGGGCTGTAACAAGAGAGTTTATAG; TTTTCCCACAATTACAGGTCTACCATTTCAGCTTCAATGGAGATAGTGGCTCTGCTCCTA; CCTCTCAAGATACATTTACAAGACTGAGGATCAGGTCTTCTCACTGGATGGCATGTGAGG; GAAGGGGACGGTCGGAGGAAAAGAAGGTAGAAAGCTTAAAATGGAAGTCAGTGTGGCCAC; ATCTCCCATTAGCTCTAGCATGAAACCTGTACAGACAATGTTTGTTTCTTTTGTAAAAAG; CAGTAAGTTATGCCCAGTAACTAAATGAATTCAAAATGGCCAAGACAAAGAAAACTAAGA; AAGATTTTGCCTTCCCTCTCCTACCAGCTATGGAGCACAGCATGTTGGGAGATGAACAGG; GAAAAGACCAAGGTAAGGAGCCTGNGAGGGAAGGTATCAACATTTTAAACTGAACTAAAA; ATAAAAGTATAAATGAGTTGGATTTAGGGTTAGATCAGTAAGACATGATTCTTACTGAAC; AGAAGTTTTTAGTATCTGTCTGCATTTTGGGTAGATTTTCAACATCTTGATGTAACTAAG; ACACACTTCCAACAGAGCCACTAGGATAACCCCACTGAAGCGCTTATGGAGTAAAGTGAT; GTAAGCGACCAGNCAGCAGTCCACTGCTCCTATAGATTGGGTCTNCCCTTCCTCTTNNCT; TCCTTCCTTNCTTTCTGGCTCCCCTGAGAAACTACATTTTTGTATTCTCTGGAGAAGACC; CCGAAATACCAACCCCTTAAGTGTGGGACCGTGGAAGAAACATGTGTGGAATGGGAAGAA; TTCCCCACTAAATGCACCACCCCGGATTGAGGCTGGTTACCTTTGTCCTTTTTGATAAGA; A;
• Restriction Sites: NotI-NotI
• ACCN: NM_177559
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_177559.2, NP_808227.1
• RefSeq Size: 2849 bp
• RefSeq ORF: 1176 bp
• Locus ID: 1457
• UniProt ID: P68400
• Cytogenetics: 20p13
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adherens junction, Tight junction, Wnt signaling pathway
• Summary: Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. Multiple transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Apr 2018]; Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, 4 and 5 encode the same protein.