PPP1R16B (NM_015568) Human Untagged Clone

SKU SC106948

PPP1R16B (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 16B (PPP1R16B), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PPP1R16B
• Synonyms: ANKRD4; TIMAP
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106948 sequence for NM_015568 edited (data generated by NextGen Sequencing); ATGGCCAGTCACGTGGACCTGCTGACGGAGCTGCAGCTGCTGGAGAAGGTGCCCACGCTG; GAGCGGCTGCGGGCTGCCCAGAAGCGCCGGGCCCAGCAGCTGAAGAAATGGGCACAGTAC; GAGCAGGACTTGCAGCACCGCAAGCGAAAGCATGAGCGGAAGCGCAGCACGGGCGGCCGC; CGCAAGAAAGTGTCCTTCGAGGCCAGCGTGGCCCTGCTGGAGGCCTCGCTGAGGAACGAC; GCCGAGGAAGTACGCTACTTCCTGAAGAATAAGGTCAGCCCTGATTTGTGCAATGAGGAC; GGACTCACAGCCCTACACCAGTGCTGCATCGACAACTTTGAGGAAATTGTGAAGCTGCTC; CTCTCCCATGGTGCCAATGTGAACGCCAAGGACAACGAGCTGTGGACACCTCTCCATGCT; GCAGCCACCTGCGGCCACATCAACCTGGTGAAGATCCTCGTTCAGTATGGGGCCGACTTG; CTTGCTGTCAACTCGGATGGGAACATGCCATATGACCTCTGCGAGGATGAACCCACCCTG; GATGTCATCGAGACCTGCATGGCATACCAGGGCATCACCCAAGAGAAAATCAACGAGATG; CGGGTGGCTCCTGAGCAGCAGATGATTGCGGACATCCACTGCATGATCGCAGCGGGCCAG; GACCTGGACTGGATAGATGCCCAGGGTGCCACACTGCTGCACATAGCTGGAGCCAATGGA; TACCTGCGGGCAGCTGAGCTCCTCCTGGACCATGGAGTGCGTGTGGATGTGAAGGACTGG; GATGGCTGGGAGCCCCTGCATGCAGCTGCCTTCTGGGGACAGATGCAGATGGCAGAGCTA; TTGGTGTCCCATGGAGCTAGTCTCAGTGCAAGGACATCCATGGATGAGATGCCAATAGAC; CTGTGTGAGGAGGAAGAGTTCAAGGTCCTGCTGCTGGAGCTAAAACACAAGCATGATGTG; ATCATGAAGTCACAGCTGAGGCACAAGTCATCCTTGAGCCGGAGGACCTCCAGCGCAGGC; AGCCGTGGGAAGGTGGTGCGGCGAGCCAGCCTGTCGGACAGGACCAACCTGTATAGGAAG; GAGTATGAGGGAGAGGCCATCCTGTGGCAGCGGAGTGCAGCTGAGGATCAGCGGACCTCC; ACCTACAACGGGGACATCAGGGAGACCAGGACAGACCAAGAGAATAAGGACCCTAACCCC; AGGCTGGAGAAGCCCGTGCTACTCTCCGAATTTCCTACCAAGATCCCACGAGGTGAACTG; GACATGCCTGTTGAGAATGGCCTCCGGGCTCCGGTCAGTGCCTACCAGTATGCGCTGGCC; AACGGGGATGTCTGGAAGGTGCATGAGGTGCCTGACTACAGCATGGCCTATGGCAACCCT; GGCGTGGCCGACGCCACCCCGCCCTGGAGCAGCTACAAGGAACAGAGCCCTCAGACGCTT; CTGGAGCTGAAGCGGCAGCGGGCTGCAGCCAAGCTGCTCAGCCACCCCTTCCTTAGCACA; CACCTGGGCAGCAGCATGGCCAGGACGGGCGAGAGTAGCAGTGAAGGCAAGGCCCCCTTG; ATCGGAGGCAGAACTTCACCGTACAGCAGCAATGGGACCTCGGTATATTACACGGTCACC; AGCGGAGATCCCCCACTCTTAAAGTTCAAGGCCCCCATAGAGGAGATGGAGGAGAAGGTG; CATGGCTGTTGCCGTATCTCCTAG; ; Clone variation with respect to NM_015568.2; 906 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015568 unedited; TATACCCCGCCCGTTGCCGCTTTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC; AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG; AATTCGGCACGAGGGCGGGCCACACCATGAGGCCCCAGCCCCACCAGAGGCCCCGCGCTG; CCCTGGCCCCCGGTGCACCGTGCTAGCCCCCAGCCAGGGCGTTGGGGAGGGCGGTGGCCA; TGGCCAGTCACGTGGACCTGCTGACGGAGCTGCAGCTGCTGGAGAAGGTGCCCACGCTGG; AGCGGCTGCGGGCTGCCCAGAAGCGCCGGGCCCAGCAGCTGAAGAAATGGGCACAGTACG; AGCAGGACTTGCAGCACCGCAAGCGAAAGCATGAGCGGAAGCGCAGCACGGGCGGCCGCC; GCAAGAAAGTGTCCTTCGAGGCCAGCGTGGCCCTGCTGGAGGCCTCGCTGAGGAACGACG; CCGAGGAAGTACGCTACTTCCTGAAGAATAAGGTCAGCCCTGATTTGTGCAATGAGGACG; GACTCACAGCCCTACACCAGTGCTGCATCGACAACTTTGAGGAAATTGTGAAGCTGCTCC; TCTCCCATGGTGCCAATGTGAACGCCAAGGACAACGAGCTGTGGACACCTCTCCATGCTG; CAGCCACCTGCGGCCACATCAACCTGGTGAAGATCCTCGTTCAGTATGGGGCCGACTTGC; TTGCTGTCAACTCGGATGGGAACATGCCATATGACCTCTGCGAGGATGAACCCACCCTGG; ATGTCATCGAGACCTGCATGGCATACCAGGGCATCACCCAAGAAGAAATCAACGAGAATG; CGGNTGGCTCCTGAGCAGCAGATGATTGCGGACATCCACTGCATGATCGCAGCGGGCCCA; GACCTGGACTGGATAGATGCCAGGGTGCCACACTGCTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015568 unedited; GGTACTTCTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTT; ATTAAAAGGAATTTAATAATTTTAATTAAAAACTCTTAACAGCGAATTCTGTCACAGGCC; TTGGCAGCTGGAAGCAACTCCAGGGACATGTGCCCCCTTTGGGATGTGGCTGAGCCACAG; TTTTGGACCCAAGGCCCACCGGTGCCGCGTGGCAAGGGGCTTCTTGGAAGTTACCTCAGT; ACGATATCGAAGATCTGCGAAGATTTTTTTTAAAAAAGTTTTAAATATATTAGACTCTCT; TGATTGCCATTTTTACACAAAGCTGCTGATACAGTATACAGAGTTTTAATTTTTTTTCAC; ATAAAATACATTTTTTTAAGTGTTCTCTGTACACCAGTGGCTTCTGGTCTGGTGAATTAT; TGCGGAGGGCTGGGGGTCTGCAGACTTTTCCTGGGTGTGCAGAGCCAGGCCCCGCACGGT; GGGACTTGCAATGCCCATGGGAAACACGCCCTCTGGCCAACAAAAGAGACACTGTTGGGG; GAGGGGGGTTGTATTTTTGTCCCCGGGGGGATGTTTTTTAAAAGTATTCCTGACCCATGG; GACCCTTGGTCTTATTGTCCCTTGGGCCGGGGGGGACCCTCCCCCATTTTCCCAATTTCG; AGAGGTAAATAACCCTTTTTTTTGGGGTTTTTTAAAAGGGGGCCCCCTCTTTAAAAAGAC; CCCCCCTTCCCCCTTTTTTTAAAAAAAAACAAGGGTTGTGGAAGAAAACCTCCTGGCGGG; GGAGGGGATTTTTGCACACCACAACCCCCAATAGTGTGTTTTTTTGTTTTTATAAACCCC; CCACCCCGGGGGGGGGGGGGAAGAGGAGACCCCACTCCTTTTGGCTTTTTTAAACCCCCT; GGAAAAAGAGAAAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_015568
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015568.2, NP_056383.1
• RefSeq Size: 6251 bp
• RefSeq ORF: 1704 bp
• Locus ID: 26051
• UniProt ID: Q96T49
• Cytogenetics: 20q11.23
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene is membrane-associated and contains five ankyrin repeats, a protein phosphatase-1-interacting domain, and a carboxy-terminal CAAX box domain. Synthesis of the encoded protein is inhibited by transforming growth factor beta-1. The protein may bind to the membrane through its CAAX box domain and may act as a signaling molecule through interaction with protein phosphatase-1. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).