Lamin B2 (LMNB2) (BC006551) Human Untagged Clone

SKU SC106163

LMNB2 (untagged)-Human lamin B2 (cDNA clone MGC:2538 IMAGE:2961134), complete cds

Product Data

• Type: Human Untagged Clone
• Target Symbol: Lamin B2
• Synonyms: LAMB2; lamin B2; LMN2; MGC2721
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106163 sequence for BC006551 edited (data generated by NextGen Sequencing); ATGGCCACGCCGCTGCCCGGCCGCGCGGGCGGGCCCGCCACGCCGCTGTCGCCCACGCGC; CTGTCGCGGCTGCAGGAGAAGGAGGAGCTGCGCGAGCTCAACGACCGCCTGGCGCACTAC; ATCGACCGCGTCCGCGCGCTGGAGCTGGAGAACGACCGGCTCCTGCTCAAGATCTCAGAG; AAGGAGGAGGTGACCACGCGCGAGGTGAGTGGCATCAAGGCGCTGTACGAGTCGGAGCTG; GCCGATGCCCGGAGAGTCCTGGATGAGACGGCTCGAGAGCGTGCCCGGCTGCAGATAGAG; ATTGGGAAGCTGAGGGCAGAGTTGGACGAGGTCAACAAGAGCGCCAAGAAGAGGGAGGGC; GAGCTTACGGTGGCCCAGGGCCGTGTGAAGGACCTGGAGTCCCTGTTCCACCGGAGCGAG; GTGGAGCTGGCAGCTGCCCTCAGCGACAAGCGCGGCCTGGAGAGTGACGTGGCTGAGCTG; CGGGCCCAGCTGGCCAAGGCCGAGGACGGTCATGCAGTGGCCAAAAAGCAGCTGGAGAAG; GAGACGCTGATGCGTGTGGACCTGGAGAACCGCTGCCAGAGCCTGCAGGAGGAGCTGGAC; TTCCGGAAGAGTGTGTTCGAGGAGGAGGTGCGGGAGACGCGGCGGCGGCACGAGCGGCGC; CTGGTGGAGGTGGACAGCAGCCGGCAGCAGGAGTACGACTTCAAGATGGCACAGGCGCTG; GAGGAGCTGCGGAGCCAGCACGACGAGCAAGTGCGGCTCTACAAGCTGGAGCTGGAGCAG; ACCTACCAGGCCAAGCTGGACAGCGCCAAGCTGAGCTCTGACCAGAACGACAAGGCGGCC; AGTGCGGCTCGCGAGGAGCTGAAGGAGGCCCGCATGCGCCTGGAGTCCCTCAGCTACCAG; CTCTCCGGCCTCCAGAAGCAGGCCAGTGCCGCTGAAGATCGCATTCGGGAGCTGGAGGAG; GCCATGGCCGGGGAGCGGGACAAGTTCCGGAAGATGCTGGACGCCAAGGAGCAGGAGATG; ACGGAGATGCGGGACGTGATGCAGCAGCAGCTGGCCGAGTACCAGGAGCTGCTGGACGTG; AAGCTGGCCCTGGACATGGAGATCAACGCCTACCGGAAGCTCCTGGAGGGCGAGGAGGAG; AGGCTGAAGCTGTCCCCCAGCCCATCCTCGCGCGTCACCGTCTCACGAGCCACCTCGAGC; AGCAGCGGCAGCTTGTCCGCCACCGGGCGCCTGGGCCGCAGTAAGCGGAAGCGGCTGGAG; GTGGAGGAGCCCTTGGGCAGCGGCCCAAGCGTCCTGGGCACGGGCACGGGTGGCAGCGGT; GGCTTCCACCTGGCCCAGCAGGCCTCGGCCTCGGGCAGCGTCAGCATCGAGGAGATCGAC; CTGGAGGGCAAGTTTGTGCAGCTCAAGAACAACTCGGACAAGGATCAGTCTCTGGGGAAC; TGGAGAATCAAGAGGCAGGTCTTGGAGGGGGAGGAGATCGCCTACAAGTTCACGCCCAAG; TACATCCTGCGCGCCGGCCAGATGGTCACGGTGTGGGCAGCTGGTGCGGGGGTGGCCCAC; AGCCCCCCCTCGACGCTGGTGTGGAAGGGCCAGAGCAGCTGGGGCACGGGCGAGAGCTTC; CGCACCGTCCTGGTTAACGCGGATGGCGAGGAAGTGGCCATGAGGACTGTGAAGAAGTCC; TCGGTGATGCGTGAGAATGAGAATGGGGAGGAAGAGGAGGAGGAAGCCGAGTTTGGCGAG; GAGGATCTTTTCCACCAACAGGGGGACCCGAGGACCACCTCAAGAGGCTGCTACGTGATG; TGA; ; Clone variation with respect to BC006551.2; 1356 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC006551 unedited; ACGTCANCATTTGTATACGACNACTATAGGGCGGCCGCGATTCGGCACCAGGGATTGAAT; GAGCCCGCCGAGCCCGGGCCGCCGTCGGGAGCAGCGCATGCCGCGAGCCGCCGCCACCAT; GGCCACGCCGCTGCCCGGCCGCGCGGGCGGGCCCGCCACGCCGCTGTCGCCCACGCGCCT; GTCGCGGCTGCAGGAGAAGGAGGAGCTGCGCGAGCTCAACGACCGCCTGGCGCACTACAT; CGACCGCGTCCGCGCGCTGGAGCTGGAGAACGACCGGCTCCTGCTCAAGATCTCAGAGAA; GGAGGAGGTGACCACGCGCGAGGTGAGTGGCATCAAGGCGCTGTACGAGTCGGAGCTGGC; CGATGCCCGGAGAGTCCTGGATGATACGGCTCGAGAGCGTGCCCGGCTGCAGATAGAGAT; TGGGAAGCTGAGGGCAGAGTTGGACGAGGTCAACAAGAGCGCCAAGAAGAGGGAGGGCGA; GCTTACGGTGGCCCAGGGCCGTGTGAAGGACCTGGAGTCCCTGTTCCACCGGAGCGAGGT; GGAGCTGGCAGCTGCCCTCATCGACAAGCGCGGCCTGGAGAGTGACGTGGCTGATCTGCG; GGCCCAGCTGGCCAAGGCCGATGACGGTCATGCAGTGGCCAAAAAGCAGCTGGAGAATGA; GACGCTGATGCGTGTGGACCTGGAGAACCGCTGCCAGAGCCTGCATGATGAGCTGGACTT; CCGGAATATTGTGTTCNATGATGATGTGCGGGATACNCNGCGGCGGCACGATCGGCGCCT; GGTGGATGTGGACAGCATCCGGCAGCATGAGTACGACTTCAAGATGCACATGCGCTGGAT; GATCTGCNGAGCCAGCACGACNAGCCAGTGCGGCTCTACAGCTGGAGCTGGAGCAGACCT; ACCAGCCCAGCTGGCAGCGCCAGCTGAGCTCTGACAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC006551 unedited; CTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTGATATAAAAATAGTTTTCAGTGGCTC; TGGGTAAAGAAAGTGTGTGGATGAAGAGTGTGGGTTCACATCACGTAGCAGCCTCTTGAG; GTGGTCCTCGGGTCCCCCTGTTGGTGGAAAAGATCCTCCTCGCCAAACTCGGCTTCCTCC; TCCTCTTCCTCCCCATTCTCATTCTCACGCATCACCGAGGACTTCTTCACAGTCCTCATG; GCCACTTCCTCGCCATCCGCGTTAACCAGGACGGTGCGGAAGCTCTCGCCCGTGCCCCAG; CTGCTCTGGCCCTTCCACACCAGCGTCGAGGGGGGGCTGTGGGCCACCCCCGCACCAGCT; GCCCACACCGTGACCATCTGGCCGGCGCGCAAGATGTACTTGGGCGTGAACTTGTAGGCG; ATCTCCTCCCCCTCCAAGACCTGCCTCTTGATTCTCCAGTTCCCCAGAGACTGATCCTTG; TCCGAGTTGTTCTTGAGCTGCACAAACTTGCCCTCCAGGTCGATCTCCTCGATGCTGACG; CTGCCCGAGGCCGAGGCCTGCTGGGCCAGGTGGAAGCCACCGCTGCCACCCGTGCCCGTG; CCCAGGACGCTTGGGCCGCTGCCCAAGGGCTCCTCCACCTCCAGCCGCTTCCGCTTACTG; CGGCCCAAGCGCCCGGTGGCGGACAAGCTGCCGCTGCTGCTCGAGGTGGCTCGTGAGACG; GTGACGCGCCGAGATGGGCTGGGGGACAGCTTTAGCCTCTCCTTCTTGCCCTCCAGGAGC; TTCCGGTAGCGTTGATCTCCATGTCCAAGG;
• Restriction Sites: NotI-NotI
• ACCN: BC006551
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC006551.2, AAH06551.1
• RefSeq Size: 1943 bp
• RefSeq ORF: 1803 bp
• Locus ID: 84823
• Cytogenetics: 19p13.3
• Domains: filament, IF_tail
• Summary: This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy. [provided by RefSeq, May 2012]