DCTN5 (NM_032486) Human Untagged Clone

SKU SC104880

DCTN5 (untagged)-Human dynactin 5 (p25) (DCTN5), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: DCTN5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_032486, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGAGTTGGGCGAGCTGCTCTACAACAAGTCTGAGTACATCGAGACGGCATCTGGGAACAAAGTCAGTC; GCCAGTCAGTGTTGTGTGGAAGCCAGAACATCGTTCTCAATGGCAAGACCATTGTGATGAATGACTGTAT; TATCCGAGGGGATCTGGCAAATGTAAGAGTTGGACGTCATTGTGTTGTGAAAAGTCGTAGTGTCATAAGG; CCACCATTCAAGAAGTTCAGCAAAGGTGTTGCATTCTTTCCTTTACATATTGGAGACCATGTCTTTATTG; AGGAAGATTGTGTGGTCAACGCAGCACAGATTGGTTCCTATGTTCATGTTGGGAAGAACTGTGTGATTGG; GCGCCGATGTGTGTTGAAAGACTGCTGCAAAATTCTTGACAACACAGTATTACCTCCAGAAACTGTGGTT; CCACCATTCACTGTCTTCTCAGGCTGCCCAGGACTCTTCTCAGGGGAGCTCCCGGAGTGCACTCAGGAGC; TGATGATTGACGTCACCAAGAGCTACTACCAGAAGTTTTTGCCCCTGACGCAAGTCTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032486 unedited; TTCAAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGCTGAAAG; ACTGACCGACTGACTCTGACAGGATCCGGGGCTGAGGGAAGGAGGCGGCGGCCATGGAGT; TGGGCGAGCTGCTCTACAACAAGTCTGAGTACATCGAGACGGCATCTGGGAACAAAGTCA; GTCGCCAGTCAGTGTTGTGTGGAAGCCAGAACATCGTTCTCAATGGCAAGACCATTGTGA; TGAATGACTGTATTATCCGAGGGGATCTGGCAAATGTAAGAGTTGGACGTCATTGTGTTG; TGAAAAGTCGTAGTGTCATAAGGCCACCATTCAAGAAGTTCAGCAAAGGTGTTGCATTCT; TTCCTTTACATATTGGAGACCATGTCTTTATTGAGGAAGATTGTGTGGTCAACGCAGCAC; AGATTGGTTCCTATGTTCATGTTGGGAAGAACTGTGTGATTGGGCGCCGATGTGTGTTGA; AAGACTGCTGCAAAATTCTTGACAACACAGTATTACCTCCGGAAACTGTGGTTCCACCAT; TCACTGTCTTCTCAGGCTGCCCAGGACTCTTCTCAGGGGAGCTCCCGGAGTGCACTCAGG; AGCTGATGATTGACGTCACCAAGAGCTACTACCAGAAGTTNTTGCCCCTGACGCAAGTCT; AGCATCTCTGCCTCATGTCTTGGATCTGCTTGAGCTCTAAGATGAACCTGGGGACAAAGT; GAGCCAGTCAGCACCTACAAAGAGCTTTTGTGTCTTTGACATCTACCACCCTTCTCCTTT; TAAAAATTTCTTTAAATTTCTCAATCTCCAGGCTCTAAGTGCTTAAGAATTACTTACAGA; CAGACCATCTGGAGGAGCTGGCTTCAAATGCTGGGCTTACACCTTATCTTATGACAGTCC; CTTTGTCCATTATCTGTGGGACCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032486 unedited; ATGCACCGCGGCACGCAATCTAGTGTCGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTCCAAACAAAAAACCATCTCCAAAGGTTTTTTATTGAACACCCATTTTGGCTA; GGCAATATCCTCCCCCTGCCCTTTAATCCAGGCTAAGGTACCCCCAGGGGAGTATCCTCA; AAAGGCAACTCCCAAAACCAGGATTATTGAAAAATTGGGCAAAGGGTAAGGGACAGCAGG; GAAACGGAGGAAAATGAAAACCCCAGGGAAAGAGGAAAGGCCTGAACTGGACAGCTGATG; CTTTGTCCTGCCCAGCACCCATTCGTCCTTTTTTAAGGTAATATCATTTGCCACCACAAC; CACCAGCACCAACTTTAATTTTTTGGGGGTACATGCCAGGCCTGTCCATTTGGGGTATTC; CATCTTCCTGGCCACAATGATGACTTGAGGCTGGATACCTTCCTCGTCGGGACCAATGAA; AACCAATTCCAGCAGTTCTGCCAGCAAAGGGGAGCTCTTTTTATAAATAACTGGGGCTGG; GGGGCCACACTGTGAAGCCCCAAAATAAAGCCAACTCAAATGAAAGCAGACCTGAAACCC; AAAAAAAAGATCCCTGTTTGGAGCTCTGGGATGCACCCTGAACCCATTCTACCCATTCTT; TTTAAGCTACGGGAACCATTAAATTCCCTTTGCCCCAAGGGACCGGGAAAGAGTCCCACC; CAGGCCCTCCCATCGGGTTGGTCTTTTGGAAAGATTTGGGGACAAGGGAATTTTATTCCC; AGGGTTTAAAAAGAATGGGGGGGGACGGCCTGTCCAAGGCCCGGGGGGGAAACCCCCTGG; GAACGGATCCACTCCGCTTTCTTGGAACACCCCACGAATGGAAAACATTTGGAAACTGGG; ACCAATCTATAACCCCATGTTCGGAAAACTTTGGTTTGTCTTCCACCCCTTGTTTGGAAA; AAAAGGCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_032486
• Insert Size: 3590 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032486.2, NP_115875.1
• RefSeq Size: 3377 bp
• RefSeq ORF: 549 bp
• Locus ID: 84516
• UniProt ID: Q9BTE1
• Cytogenetics: 16p12.2
• Domains: hexapep
• Summary: This gene encodes a subunit of dynactin, a component of the cytoplasmic dynein motor machinery involved in minus-end-directed transport. The encoded protein is a component of the pointed-end subcomplex and is thought to bind membranous cargo. A pseudogene of this gene is located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]; Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.