GFM2 (NM_032380) Human Untagged Clone

SKU SC104725

GFM2 (untagged)-Human G elongation factor, mitochondrial 2 (GFM2), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: GFM2
• Synonyms: EF-G2mt; EFG2; hEFG2; mEF-G 2; MRRF2; MST027; MSTP027; RRF; RRF2; RRF2mt
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC104725 sequence for NM_032380 edited (data generated by NextGen Sequencing); ATGTTGACCAACTTGAGGATATTTGCAATGAGTCATCAGACAATACCCAGTGTGTATATT; AATAATATATGCTGCTATAAAATAAGAGCAAGTTTAAAAAGATTAAAGCCACATGTGCCG; CTTGGAAGAAATTGCAGTTCTCTACCAGGCTTAATAGGAAATGATATCAAATCCCTTCAT; TCCATCATCAATCCTCCCATAGCTAAAATCCGTAATATTGGAATTATGGCTCATATTGAT; GCAGGCAAAACTACCACCACAGAAAGAATATTGTACTATTCCGGATATACAAGATCACTG; GGAGATGTTGATGATGGAGACACAGTGACAGATTTCATGGCCCAAGAGCGAGAAAGAGGC; ATTACTATTCAATCAGCTGCTGTTACATTTGATTGGAAAGGTTATAGAGTCAATCTAATT; GATACACCAGGTCATGTGGACTTTACCTTGGAGGTTGAGCGGTGCCTAAGAGTGTTGGAT; GGTGCAGTGGCTGTATTTGATGCCTCTGCTGGTGTAGAGGCCCAGACTCTCACAGTATGG; AGGCAAGCTGATAAACACAATATACCTCGAATCTGTTTTTTAAACAAGATGGACAAAACT; GGAGCAAGCTTTAAGTATGCAGTTGAAAGCATCAGAGAGAAGTTAAAGGCAAAGCCTTTG; CTTTTACAGTTACCAATTGGTGAAGCCAAAACTTTCAAAGGAGTGGTGGATGTAGTAATG; AAAGAAAAACTTCTTTGGAATTGCAATTCAAATGATGGAAAAGACTTTGAGAGAAAGCCC; CTCTTGGAAATGAATGATCCTGAATTGCTGAAGGAAACAACTGAAGCAAGGAATGCCTTA; ATTGAACAAGTTGCAGATTTGGATGATGAATTTGCTGACTTGGTTTTAGAAGAATTTAGT; GAGAATTTTGATTTGTTACCAGCTGAAAAGCTACAGACTGCAATACATAGAGTGACACTA; GCTCAGACAGCAGTGCCTGTGCTTTGTGGAAGTGCCCTGAAAAACAAAGGGATACAGCCC; TTGTTAGATGCTGTTACTATGTACTTACCTTCACCTGAAGAGCGTAACTATGAATTTCTG; CAGTGGTATAAGGATGACTTATGTGCATTGGCATTTAAAGTTCTCCATGACAAGCAGCGA; GGACCACTGGTTTTTATGCGCATTTACTCAGGCACTATAAAACCCCAGTTGGCCATTCAT; AATATTAATGGAAACTGCACGGAGAGAATAAGTCGTCTGCTTTTGCCGTTTGCTGACCAA; CATGTAGAAATCCCTTCATTGACTGCTGGTAACATTGCTTTGACTGTTGGGCTTAAACAT; ACTGCCACTGGAGACACCATTGTCTCATCCAAGTCCAGTGCATTAGCTGCAGCTCGTAGA; GCCGAACGGGAGGGAGAAAAGAAGCACAGACAAAACAATGAAGCAGAGAGACTTTTATTG; GCTGGAGTGGAGATTCCAGAACCTGTTTTCTTCTGTACCATAGAACCCCCATCACTGTCT; AAGCAGCCAGATTTGGAACATGCGTTGAAATGTCTTCAGCGTGAAGATCCCAGTTTGAAA; GTGAGGCTAGATCCTGACTCTGGACAAACTGTTCTGTGTGGTATGGGGGAGTTACATATA; GAGATTATTCATGATCGAATCAAGAGGGAATATGGACTGGAGACCTATCTCGGGCCTCTC; CAGGTGGCATATCGAGAGACCATCCTAAACTCAGTTCGTGCCACAGATACCTTAGATAGA; ACTTTAGGAGACAAAAGGCATCTTGTGACTGTAGAAGTGGAAGCAAGGCCAATTGAAACA; TCATCTGTTATGCCTGTGATTGAGTTTGAGTATGCTGAAAGTATCAATGAAGGCCTTTTG; AAGGTCTCCCAAGAGGCCATTGAAAATGGAATTCACAGCGCATGTCTCCAAGGACCATTG; CTTGGATCCCCAATTCAGGATGTAGCAATTACTTTACATTCCCTGACAATTCATCCTGGC; ACCTCCACAACTATGATTTCTGCCTGTGTCTCAAGATGCGTGCAAAAGGCTCTGAAGAAA; GCTGATAAGCAAGTTTTGGAGCCTCTGATGAATCTTGAGGTTACAGTAGCTAGAGATTAT; CTCAGCCCTGTCCTGGCAGATCTGGCACAAAGAAGAGGAAACATTCAGGAAATTCAGACT; CGCCAGGACAACAAAGTTGTTATTGGATTTGTTCCCTTAGCAGAAATTATGGGTTATTCA; ACTGTGCTTCGAACGCTAACATCAGGCTCAGCTACTTTTGCCTTAGAACTATCTACTTAT; CAAGCCATGAATCCTCAAGATCAAAATACACTGCTCAACCGGAGAAGTGGTTTGACCTAA; ; Clone variation with respect to NM_032380.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032380 unedited; TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCGGAAGTGTG; GAGGTGTGGCTGCCCGAGGCGGTAGAGCGGAGAAAGCCTATTTTCTTTTCGTTTAGATAC; ATTGCCTTTTGCCTAGGCTGGCGTCGAGACTTGAGGCCGTTGCAGACTTTGGCGCGGCTC; GCGCCTCCTGCTTCAAGAGCCCAGCGGTGAGAGCTGGCCTGCGGCACGCGGCCTAATGCC; AGACAGTAACAGTTTGGAGGATCAAGATGTTGACCAACTTGAGGATATTTGCAATGAGTC; ATCAGACAATACCCAGTGTGTATATTAATAATATATGCTGCTATAAAATAAGAGCAAGTT; TAAAAAGATTAAAGCCACATGTGCCGCTTGGAAGAAATTGCAGTTCTCTACCAGGCTTAA; TAGGAAATGATATCAAATCCCTTCATTCCATCATCAGTCCTCCCATAGCTAAAATCCGTA; ATATTGGAATTATGGCTCATATTGATGCAGGCAAAACTACCACCACAGAAAGAATATTGT; ACTATTCCGGATATACAAGATCACTGGGAGATGTTGATGATGGAGACACAGTGACAGATT; TCATGGCCCAAGAGCGAGAAAGAGGCATTACTATTCAATCAGCTGCTGTTACATTTGATT; GGAAAGGTTATAGAGTCAATCTAATTGATACACCAGNTCATGTGGACTTTACTTGGGAGG; GTGAGCGGTGCCTAAAATGNTNGATGGNNGCANTGGCTGTATTTGATGCTCTGCTGTNGT; ANAGGCCAGACTCTCCAGTNATGGAGGCAGCTGATAACACATATACCTCGAATCTGTTTT; TAAACAGATGGACANACTGGAGCGNCTTTANNTATGCAGNTGAAGCTCNANAAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032380 unedited; TGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTTTTTGAATAAAA; TATTTTTATTGATTGAACCTTTGACCCTCTATCTTATTACATTTAGAGGCCTGGCATCTT; TCTTGTGAGACAAGCTTAAGGACACAAAAGAAACACAACTTTGTGATACCACTCTTCTGA; AGGCTACTTATAGTAATAACTTCATAGATGAACAGCATGGAATTAGGTACAGTGGCTTTA; AACATCAAAGCACATTTCTCATTATATAAATTAAAACGGGTGGCTCCAGTGCCACTATCA; AAGCTTAAGTTATATTCTTTTATCTAGTTCTCTGAATGTACTGAAACAGTACTTTATTCG; TCCATAAATAAAGCAATANAAATTGGTCTTACTGAAAATGAAGTAGCTAGGTGCTCCTGA; AATTCTCTCCAAAAACTAAAACATTTAGGTCAAACCACTTNCTCTGGTTGAGCAGTGTAT; TTTGATCTTGAGGATTCATGGCTTGATAAGTAGATAGTNCTTAAGCAAAAGTAGCTGAGC; CTGAATGTAGCGTTCGAGCACAGTTGAATAACCCATAATTTCTGCTAGGGAACAAATNNC; ATACAACTTTGTTGTCCTGGCGAGTCTGAATTTCCTGAATGGTTTCCTCTTCTTTTGTGC; ANAATCTGCCANGAACAGGGCTGAAATAATCTTTGCTACTGAACCCTCAAGATCATCAGA; GGCTCAAAACTTGCTATCAGCTTCTTTAAAACTTTTGCCGCATTTTGGAACCAGCAGATT; ATAGTGTGAAGGGCCAGATGAATGGCAGGAATGAAATAATTGGTCATCTGGATGGGGATC; CACCATGGTCCTGGAAATGCCCTGGATTCATTTTCATGGCTCTGGGAAACTTAAGGCTTT; TGTACTTAAATACAATACGGCTAAAAGAGGTTAATGGCCTGTTCCTTAAGACAAAGCTTT; GTTCTAATTATTAGGTTGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_032380
• Insert Size: 3110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032380.3, NP_115756.2
• RefSeq Size: 3111 bp
• RefSeq ORF: 2340 bp
• Locus ID: 84340
• UniProt ID: Q969S9
• Cytogenetics: 5q13.3
• Domains: EFG_C, EFG_IV, GTP_EFTU, GTP_EFTU_D2
• Summary: Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors, which is a GTPase that plays a role at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA . Its role in the regulation of normal mitochondrial function and in disease states attributed to mitochondrial dysfunction is not known. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013]; Transcript Variant: This variant (1) lacks an alternate exon in the 5' coding region and initiates translation at a downstream start codon, compared to variant 4. It encodes isoform 1 which is shorter at the N-terminus, compared to isoform 4.