Cytokeratin 4 (KRT4) (NM_002272) Human Untagged Clone

SKU SC102891

KRT4 (untagged)-Human keratin 4 (KRT4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cytokeratin 4
• Synonyms: CK-4; CK4; CYK4; K4; WSN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC102891 sequence for NM_002272 edited (data generated by NextGen Sequencing); ATGATTGCCAGACAGCAGTGTGTCCGAGGCGGGCCCCGGGGCTTCAGCTGTGGCTCGGCC; ATTGTAGGNGGNNNNNNNAGAGGTGCCTTCAGCTCAGTCTCCATGTCTGGAGGTGCTGGC; CGATGCTCTTCTGGGGGATTTGGCAGCAGAAGCCTCTACAACCTCAGGGGGAACAAAAGC; ATCTCCATGAGTGTGGCTGGGTCACGACAAGGTGCCTGCTTTGGGGGTGCTGGAGGCTTT; GGCACTGGTGGCTTTGGTGGTGGATTTGGGGGCTCCTTCAGTGGTAAGGGTGGCCCTGGC; TTCCCCGTCTGCCCCGCTGGGGGAATTCAGGAGGTCACCATCAACCAGAGCTTGCTCACC; CCCCTCCACGTGGAGATTGACCCTGAGATCCAGAAAGTCCGGACGGAAGAGCGCGAACAG; ATCAAGCTCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCAGTTCTTAGAGCAA; CAGAATAAGGTCCTGGAGACCAAATGGAACCTGCTCCAGCAGCAGACGACCACCACCTCC; AGCAAAAACCTTGAGCCCCTCTTTGAGACCTACCTCAGTGTCCTGAGGAAGCAGCTAGAT; ACCTTGGGCAATGACAAAGGGCGCCTGCAGTCTGAGCTGAAGACCATGCAGGACAGCGTG; GAGGACTTCAAGACTAAGTATGAAGAGGAGATCAACAAACGCACAGCAGCCGAGAATGAC; TTTGTGGTCCTAAAGAAGGACGTGGATGCTGCCTACCTGAACAAGGTGGAGTTGGAGGCC; AAGGTGGACAGTCTTAATGACGAGATCAACTTCCTGAAGGTCCTCTATGATGCGGAGCTG; TCCCAGATGCAGACCCATGTCAGCGACACGTCCGTGGTCCTTTCCATGGACAACAACCGC; AACCTGGACCTGGACAGCATTATTGCCGAGGTCCGTGCCCAGTACGAGGAGATTGCCCAG; AGGAGCAAGGCTGAGGCTGAAGCCCTGTACCAGACCAAGGTCCAGCAGCTCCAGATCTCG; GTTGACCAACATGGTGACAACCTGAAGAACACCAAGAGTGAAATTGCAGAGCTCAACAGG; ATGATCCAGAGGCTGCGGGCAGAGATCGAGAACATCAAGAAGCAGTGCCAGACTCTTCAG; GTATCCGTGGCTGATGCAGAGCAGCGAGGTGAGAATGCCCTTAAAGATGCCCACAGCAAG; CGCGTAGAGCTGGAGGCTGCCCTGCAGCAGGCCAAGGAGGAGCTGGCACGAATGCTGCGT; GAGTACCAGGAGCTCATGAGTGTGAAGCTGGCCTTGGACATCGAGATCGCCACCTACCGC; AAACTGCTGGAGGGCGAGGAGTACAGAATGTCTGGAGAATGCCAGAGTGCCGTGAGCATC; TCTGTGGTCAGCGGTAGCACCAGCACTGGAGGCATCAGCGGAGGATTAGGAAGTGGCTCC; GGGTTTGGCCTGAGTAGTGGCTTTGGCTCCGGCTCTGGAAGTGGCTTTGGGTTTGGTGGC; AGTGTCTCTGGCAGTTCCAGCAGCAAGATCATCTCTACCACCACCCTGAACAAGAGACGA; TAG; ; Clone variation with respect to NM_002272.3; 69 c=>n;72 t=>n;73 g=>n;74 g=>n;75 c=>n;76 a=>n;77 a=>n;78 g=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002272 unedited; GGTTGCGNATTTGTATACGATCCTATAGGCGGCCCGCGATTCGGCACCAGCACTTCTCTG; ATAGCTCCCAGCTCGCTCTCTGCAGCCATGATTGCCAGACAGCAGTGTGTCCGAGGCGGG; CCCCGGGGCTTCAGCTGTGGCTCGGCCATTGTAGGCGGTGGCAAGAGAGGTGCCTTCAGC; TCAGTCTCCATGTCTGGAGGTGCTGGCCGATGCTCTTCTGGGGGATTTGGCAGCAGAAGC; CTCTACAACCTCAGGGGGAACAAAAGCATCTCCATGAGTGTGGCTGGGTCACGACAAGGT; GCCTGCTTTGGGGGTGCTGGAGGCTTTGGCACTGGTGGCTTTGGTGCCGGCGGCTTCGGA; GCTGGTTTCGGCACTGGTGGCTTTGGTGGTGGATTTGGGGGCTCCTTCAGTGGTAAGGGT; GGCCCTGGCTTCCCCGTCTGCCCCGCTGGGGGAATTCAGGAGGTCACCATCAACCAGAGC; TTGCTCACCCCCCTCCACGTGGAGATTGACCCTGAGATCCAGAAAGTCCGGACGGAAGAG; CGCGAACAGATCAACCTCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCAGTTC; TTAGAGCAACAGAATAAGGTCCTGGAGACCAAATGGAACCTGCTCCACCAGCAGACGACC; ACCACCTCCAGCANAAACCTTGAGCCCCTCTTTGAGACCTACCTCAGTGTCCTGAGGAAG; CAGCTAGATACCTTGGGCATTGACAAAGGGCGCCTGCAGTCTGAGCTGAAGACCATGCAN; GCCAGCGTGGAGGACTTCAAGACTAAGTATGGAGAGGAGATCAACAAACGCACAGCAGCC; CGAGAAGACTTTTGTGGTCCTAAAGAAAGACGGGNATGCTGCCTCACTGGACANGTTGNA; GTTGTAGGCCAGGTGGACAGTCTTATGAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002272 unedited; AACTAGCTATGCACGCGCGGCCCGTCATACTACGACCCGAGCTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTGAAGGACCAAAGCATTTTATTGAAGATTCACCTGCAGATGGA; TAACAGGGACAGAGAGGAGTACACATGGGACACTAAGTGGTTCTCATCCTAAGCTTGCAG; GGCCAAGTGCTGCCGGGTGTTGGACAAGTACTTTGGTTCTGATGTACATGGATAATACAG; GATCTAGTGGGAGATGGCATTGGACTGGGAAGGGACATATGTGACCCCAATAATTCTGGA; GAGGACACTCCTGGGCATTTTCTTCTCTGCCCATGGGAGGTGAGAGGTCAGCTGACATCC; TTCCCATTCCAGGGGGGTCACCAGGCCCAAATCAAGAAGTAGCTACCCAACTCCAAGAGG; CAGAGTCCCTGTCCCAGCACAGAAGATTATTCTGGGGCAAAGAGAGCCCATGGGATAGTG; GAGGGGATACTTGTCAGATGAGCCCCCCAACAGAGGATGGAGGTGAAGTGCAGGACCCAC; CCAGACACCAGTGCTGGCCCCAGCTGGACACCGCGAGCCTGCGGGCCCCCCTCTCCTCTA; TCGTCTCTTGTCCCGGGTGGTGGTCGAGATGACTCTCCTTGTGGAACTTGCCTACTACCT; GTCCCCAACCCCCCCCGCCACTTCCTTATCTCTTCCCCTACCCACTTTTACGCCCCCCCC; TTGTATCCCCCCCAAACACCCTCCCTTCACGCCACCCCCCCATTTTGTTTCTCCTCCCTC; TACCAACTCTCTCTCCCCCGCCCTTCTCTTATATCCCACACAACCTATTCACCCTCCCCT; TCTTCCTTTCCCTTCTCTCCTCCATTCTCAACCCCCGCCCGTTACACCCCCCCGCCCCCC; TCTCTTCACTCATAAAATCATACTTCATTCTACTTCTTCTCCCCCCCCCCATCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_002272
• Insert Size: 2170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002272.1, NP_002263.1
• RefSeq Size: 2617 bp
• RefSeq ORF: 1605 bp
• Locus ID: 3851
• UniProt ID: P19013
• Cytogenetics: 12q13.13
• Domains: filament
• Summary: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]