PSMB10 (NM_002801) Human Untagged Clone

CAT#: SC102824

PSMB10 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 10 (PSMB10)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMB10
• Synonyms: beta2i; LMP10; MECL1; PRAAS5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC102824 sequence for NM_002801 edited (data generated by NextGen Sequencing)
  ATGCTGAAGCCAGCCCTGGAGCCCCGAGGGGGCTTCTCCTTCGAGAACTGCCAAAGAAAT
  GCATCATTGGAACGCGTCCTCCCGGGGCTCAAGGTCCCTCACGCACGCAAGACCGGGACC
  ACCATCGCGGGCCTGGTGTTCCAAGACGGGGTCATTCTGGGCGCCGATACGCGAGCCACT
  AACGATTCGGTCGTGGCGGACAAGAGCTGCGAGAAGATCCACTTCATCGCCCCCAAAATC
  TACTGCTGTGGGGCTGGAGTAGCCGCGGACGCCGAGATGACCACACGGATGGTGGCGTCC
  AAGATGGAGCTACACGCGTTATCTACGGGCCGCGAGCCCCGCGTGGCCACGGTCACTCGC
  ATCCTGCGCCAGACGCTCTTCAGGTACCAGGGCCACGTGGGTGCATCGCTGATCGTGGGC
  GGCGTAGACCTGACTGGACCGCAGCTCTACGGTGTGCATCCCCATGGCTCCTACAGCCGT
  CTGCCCTTCACAGCCCTGGGCTCTGGTCAGGACGCGGCCCTGGCGGTGCTAGAAGACCGG
  TTCCAGCCGAACATGACGCTGGAGGCTGCTCAGGGGCTGCTGGTGGAAGCCGTCACCGCC
  GGGATCTTGGGTGACCTGGGCTCCGGGGGCAATGTGGACGCATGTGTGATCACAAAGACT
  GGCGCCAAGCTGCTGCGGACACTGAGCTCACCCACAGAGCCCGTGAAGAGGTCTGGCCGC
  TACCACTTTGTGCCTGGAACCACAGCTGTCCTGACCCAGACAGTGAAGCCACTAACCCTG
  GAGCTAGTGGAGGAAACTGTGCAGGCTATGGAGGTGGAGTAA
  
  Clone variation with respect to NM_002801.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002801 unedited
  NGGGGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGCTT
  GTCTGGCCGACTCATCCGCCCGCGACCCCTAATCCCCTCTGCCTGCCCCAAGATGCTGAA
  GCCAGCCCTGGAGCCCCGAGGGGGCTTCTCCTTCGAGAACTGCCAAAGAAATGCATCATT
  GGAACGCGTCCTCCCGGGGCTCAAGGTCCCTCACGCACGCAAGACCGGGACCACCATCGC
  GGGCCTGGTGTTCCAAGACGGGGTCATTCTGGGCGCCGATACGCGAGCCACTAACGATTC
  GGTCGTGGCGGACAAGAGCTGCGAGAAGATCCACTTCATCGCCCCCAAAATCTACTGCTG
  TGGGGCTGGAGTAGCCGCGGACGCCGAGATGACCACACGGATGGTGGCGTCCAAGATGGA
  GCTACACGCGTTATCTACGGGCCGCGAGCCCCGCGTGGCCACGGTCACTCGCATCCTGCG
  CCAGACGCTCTTCAGGTACCAGGGCCACGTGGGTGCATCGCTGATCGTGGGCGGCGTAGA
  CCTGACTGGACCGCAGCTCTACGGTGTGCATCCCCATGGCTCCTACAGCCGTCTGCCCTT
  CACAGCCCTGGGCTCTGGTCAGGACGCGGCCCTGGCGGTGCTAGAAGACCGGTTCCAGCC
  GAACATGACGCTGGAGGCTGCTCANGGGCTGCTGGTGGAAGCCGTCACCGCCGGGATCTT
  GGGTGACCTGNGCTCCGGGGGCAATGTGGACGCATGTTGTGATCACAAAGACTGGCGCCA
  AGCTGCTGCGGACACTGAGCTCACCCCANAGCCGTGAAGAAGTCTGGCCGCTACCACTTT
  GTGCCTGGAACCACAGCTGTTCTGACCCAGACAGTGAAGCCACTAACCCTGNAGCTAGTG
  GNAGGAACTGTGCAGCTATTGAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002801
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002801.2, NP_002792.1
• RefSeq Size: 1009 bp
• RefSeq ORF: 822 bp
• Locus ID: 5699
• UniProt ID: P40306
• Cytogenetics: 16q22.1
• Domains: proteasome
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008]