p21(CDKN1A) (NM_078467) Human Untagged Clone
CDKN1A (untagged)-Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 2
|Product Name||p21(CDKN1A) (NM_078467) Human Untagged Clone|
|Synonyms||CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1; SDI1; WAF1|
Fully Sequenced ORF
>OriGene ORF within SC102496 sequence for NM_078467 edited (data generated by NextGen Sequencing)
Clone variation with respect to NM_078467.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_078467 unedited
3' Read Nucleotide Sequence
>OriGene 3' read for NM_078467 unedited
|ORF Size||495 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|Product Components||The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.|
|Protein Families||Druggable Genome|
|Protein Pathways||ErbB signaling pathway, Cell cycle, p53 signaling pathway, Pathways in cancer, Glioma, Prostate cancer, Melanoma, Bladder cancer, Chronic myeloid leukemia|
|Gene Summary||This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015].
Transcript Variant: This variant (2) differs in the 5' UTR, lacks an in-frame portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 3. The resulting isoform (1) has a shorter N-terminus, compared to isoform 2. Variants 1, 2, 4 and 5 encode the same isoform 1.
|The use of this cDNA Clones has been cited in the following citations:|
The Effects of Two Polymorphisms on p21cip1 Function and Their Association with Alzheimer's Disease in a Population of European Descent
,Yates, SC;Zafar, A;Rabai, EM;Foxall, JB;Nagy, S;Morrison, KE;Clarke, C;Esiri, MM;Christie, S;Smith, AD;Nagy, Z;,
PLoS ONE Jan 2015,PubMed ID 25625488
Depletion of the Poly(C)-binding Proteins CP1 and CP2 from K562 Cells Leads to p53-independent Induction of Cyclin-dependent Kinase Inhibitor (CDKN1A) and G1 Arrest
,Shelly A. Waggoner, Gregg J. Johannes, and Stephen A. Liebhaber,
J. Biol. Chem., Apr 2009; 284: 9039 - 9049.
The Aurora Kinase Inhibitor VX-680 Induces Endoreduplication and Apoptosis Preferentially in Cells with Compromised p53-Dependent Postmitotic Checkpoint Function
,Farid Gizatullin, Yao Yao, Victor Kung, Matthew W. Harding, Massimo Loda, and Geoffrey I. Shapiro,
Cancer Research 66, 7668-7677, August 1, 2006
|cDNA Clone Resources|