SFRS2B (SRSF8) (NM_032102) Human Untagged Clone

SKU SC102448

SRSF8 (untagged)-Human serine/arginine-rich splicing factor 8 (SRSF8)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SFRS2B
• Synonyms: DSM-1; SFRS2B; SRP46
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC102448 sequence for NM_032102 edited (data generated by NextGen Sequencing); ATGAGCTGCGGCCGCCCCCCTCCCGACGTGGACGGCATGATCACCCTCAAGGTGGACAAC; CTGACCTACCGCACCTCTCCCGACAGCTTGAGGCGCGTGTTCGAGAAGTACGGGCGCGTG; GGCGACGTGTACATCCCGCGGGAGCCCCACACCAAGGCGCCCCGGGGCTTCGCTTTCGTC; CGCTTTCACGACCGGCGCGACGCCCAAGACGCCGAGGCCGCCATGGACGGGGCGGAGCTG; GACGGACGCGAGCTGCGGGTGCAGGTGGCGCGCTATGGCCGCCGGGACCTGCCCCGCAGC; CGCCAGGGAGAGCCACGCGGCAGGTCCAGAGGCGGCGGCTACGGACGGCGGAGCCGCAGC; TACGGGCGGCGGAGCCGCAGCCCCAGGCGGCGACACCGCAGCCGATCCCGGGGTCCCAGC; TGCTCCAGGTCCCGCAGCCGATCTCGCTATAGGGGTTCTCGCTATAGCCGGTCTCCCTAC; AGCCGATCTCCTTACAGCCGGTCGCGCTACAGCCGCTCTCCCTACAGCAGATCTCGCTAC; AGGGAATCTCGCTACGGCGGATCTCACTACAGCTCATCTGGTTACAGTAACTCTCGCTAC; AGCCGATATCACAGCAGCCGGTCTCACTCGAAGTCTGGGTCCTCCACTAGCTCTCGCTCT; GCATCAACCTCCAAATCGAGCTCTGCGCGACGATCCAAGTCCTCCTCGGTCTCCAGGTCT; CGCTCGCGGTCCAGGTCTTCATCTATGACCAGGAGTCCTCCCCGGGTATCCAAGAGGAAA; TCCAAGTCAAGGTCGCGATCCAAGAGGCCCCCCAAGTCTCCTGAAGAGGAAGGACAGATG; TCCTCTTAA; ; Clone variation with respect to NM_032102.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032102 unedited; GGGGTTCGAATTTGTATACGACTCCTATAGGCGGCCGCGNAATCGGCACCAGCTTCCTCT; GGGTCCTGGAGTATTTGGCCTGAAATGGGAACTCGGAAGTTGCTGCTCCAGGGCGCTCCC; TGCGGAGCTCCGCCGCCCGCCTCTCCGCCCGGCCTTTCCCGGCGTCCCCACGCGGGGCGC; AACCGCGAGAAAGAAACGCAGGTCGCACCGTCAGCGCCCAGAGCAGCGCCAGTTTCCGGG; CCCGGGCTGCTCTCGGAGCCATGAGCTGCGGCCGCCCCCCTCCCGACGTGGACGGCATGA; TCACCCTCAAGGTGGACAACCTGACCTACCGCACCTCTCCCGACAGCTTGAGGCGCGTGT; TCGAGAAGTACGGGCGCGTGGGCGACGTGTACATCCCGCGGGAGCCCCACACCAAGGCGC; CCCGGGGCTTCGCTTTCGTCCGCTTTCACGACCGGCGCGACGCCCAAGACGCCGAGGCCG; CCATGGACGGGGCGGAGCTGGACGGACGCGAGCTGCGGGTGCAGGTGGCGCGCTATGGCC; GCCGGGACCTGCCCCGCAGCCGCCAGGGAGAGCCACGCGGCAGGTCCAGAGGCGGCGGCT; ACGGACGGCGGAGCCGCAGCTACGGGCGGCGGAGCCGCAGCCCCAGGCGGCGACACCGCA; GCCGATCCCCGGGTCCCAGCTGCTCCAGGTCCCGCAGCCGATCTCGCTATAGGGGGTCTC; GCTATAGCCGGTCTCCCCTACGCCGATCTCCTTACAGCCGGTCGCGCTACAGNCGGTCTC; CCTACAGCAGATCTCGCTACAGGGAATCTCGCTACGGGCGGATCTCACTACAGCTCATCT; GGGTACAGTAACTCTCGCTACAGCCGATATCACAGCAGCCGGTCTCACTCGAGATCTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032102 unedited; NTTTTACTTTGGAACCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTCCAT; CAAAGTTTCTAATTTTATTCTGAGAAAAGAAACACCAAGAAAACAATTTTAACTACAGAA; ACAATGGTTAGCGTAAAAAATTAATAATCAAAAAGATAAACCATGTTATAAGTACTTAAC; AAACTTATAAACTATTTCTTCCATAACATTCTCCATATTTTAAGTTCATACATGTTGATA; TGATCAGATTTACAATTTTTCAGAAAAGACGGGGGAAAAGGTGTGATTATCGTCAGCTAG; ATGTGCTCACTGCATGCTCCATTATTTATCTGCAAGGCCTGGGTGACTGGAAGTGCAGTT; GACAGGCATTTTAATAAACTGGACAGCCATTTGTTCTTCAGGACAAGGCGTGTTTTCCCC; AGGAGCAATCAGAAGAAAACAGGAAGCAGCTCAGCACTCTGCACTGCAACAGGCCACCTT; AATAGCTAACCAGCATCACTCAACTGCTACGCAGCCGGGCCTGGTGCACAAAAACACAAA; AGAGATTAGACCTGTGTTTGCTAAAACCCCAGTCCTTTCACATGAAAAATCTTTATACAA; ATTTGGGTCACACTACCTTTTAGACCTGCAGGTAAATAAAATTATCCATATTAATTTACA; CCACCTTTTGTAGGTTTCTAATGAATGAAGGCTATGTTGAATCAATGTCTTGACAGCGCT; AGGCTGTGTGGAACGGCTGCCCACCTTCCTTCATACCTAGGAGGCCCTAATTAGGCCACC; ACTCTCTCATCCTAAACCCACCCGTCTTGCTATTGCATAAAGATTACCCCCTGGCTTCAA; CATCTACACCAATACGTAATTTTAGGTCCCCACCCCTCCATTCCACTAATCGCGGCCCAC; CCTACTCCGCCTATCCATCATCTTCCCCCCTTCCACACT;
• Restriction Sites: NotI-NotI
• ACCN: NM_032102
• Insert Size: 1820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032102.1, NP_115285.1
• RefSeq Size: 2186 bp
• RefSeq ORF: 849 bp
• Locus ID: 10929
• UniProt ID: Q9BRL6
• Cytogenetics: 11q21
• Domains: RRM
• Protein Pathways: Spliceosome
• Summary: This gene encodes a member of a family of proteins containing a ribonucleoprotein (RNP)-type RNA binding motif and a carboxyl-terminal arginine-serine-rich (RS) domain. The encoded protein functions as a pre-mRNA splicing factor. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]; Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.