EZH2 (NM_152998) Human Untagged Clone

CAT#: SC101257

EZH2 (untagged)-Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EZH2
• Synonyms: ENX-1; ENX1; EZH2b; KMT6; KMT6A; WVS; WVS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101257 sequence for NM_152998 edited (data generated by NextGen Sequencing)
  ATGGGCCAGACTGGGAAGAAATCTGAGAAGGGACCAGTTTGTTGGCGGAAGCGTGTAAAA
  TCAGAGTACATGCGACTGAGACAGCTCAAGAGGTTCAGACGAGCTGATGAAGTAAAGAGT
  ATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAATCTTAAACCAAGAATGG
  AAACAGCGAAGGATACAGCCTGTGCACATCCTGACTTCTGTGAGCTCATTGCGCGGGACT
  AGGGAGGTGGAAGATGAAACTGTTTTACATAACATTCCTTATATGGGAGATGAAGTTTTA
  GATCAGGATGGTACTTTCATTGAAGAACTAATAAAAAATTATGATGGGAAAGTACACGGG
  GATAGAGAATGTGGGTTTATAAATGATGAAATTTTTGTGGAGTTGGTGAATGCCCTTGGT
  CAATATAATGATGATGACGATGATGATGATGGAGACGATCCTGAAGAAAGAGAAGAAAAG
  CAGAAAGATCTGGAGGATCACCGAGATGATAAAGAAAGCCGCCCACCTCGGAAATTTCCT
  TCTGATAAAATTTTTGAAGCCATTTCCTCAATGTTTCCAGATAAGGGCACAGCAGAAGAA
  CTAAAGGAAAAATATAAAGAACTCACCGAACAGCAGCTCCCAGGCGCACTTCCTCCTGAA
  TGTACCCCCAACATAGATGGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACAC
  TCCTTTCATACGCTTTTCTGTAGGCGATGTTTTAAATATGACTGCTTCCTACATCCTTTT
  CATGCAACACCCAACACTTATAAGCGGAAGAACACAGAAACAGCTCTAGACAACAAACCT
  TGTGGACCACAGTGTTACCAGCATTTGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACC
  GCTGAGCGGATAAAGACCCCACCAAAACGTCCAGGAGGCCGCAGAAGAGGACGGCTTCCC
  AATAACAGTAGCAGGCCCAGCACCCCCACCATTAATGTGCTGGAATCAAAGGATACAGAC
  AGTGATAGGGAAGCAGGGACTGAAACGGGGGGAGAGAACAATGATAAAGAAGAAGAAGAG
  AAGAAAGATGAAACTTCGAGCTCCTCTGAAGCAAATTCTCGGTGTCAAACACCAATAAAG
  ATGAAGCCAAATATTGAACCTCCTGAGAATGTGGAGTGGAGTGGTGCTGAAGCCTCAATG
  TTTAGAGTCCTCATTGGCACTTACTATGACAATTTCTGTGCCATTGCTAGGTTAATTGGG
  ACCAAAACATGTAGACAGGTGTATGAGTTTAGAGTCAAAGAATCTAGCATCATAGCTCCA
  GCTCCCGCTGAGGATGTGGATACTCCTCCAAGGAAAAAGAAGAGGAAACACCGGTTGTGG
  GCTGCACACTGCAGAAAGATACAGCTGAAAAAGGACGGCTCCTCTAACCATGTTTACAAC
  TATCAACCCTGTGATCATCCACGGCAGCCTTGTGACAGTTCGTGCCCTTGTGTGATAGCA
  CAAAATTTTTGTGAAAAGTTTTGTCAATGTAGTTCAGAGTGTCAAAACCGCTTTCCGGGA
  TGCCGCTGCAAAGCACAGTGCAACACCAAGCAGTGCCCGTGCTACCTGGCTGTCCGAGAG
  TGTGACCCTGACCTCTGTCTTACTTGTGGAGCCGCTGACCATTGGGACAGTAAAAATGTG
  TCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGCATCTATTGCTGGCACCATCT
  GACGTGGCAGGCTGGGGGATTTTTATCAAAGATCCTGTGCAGAAAAATGAATTCATCTCA
  GAATACTGTGGAGAGATTATTTCTCAAGATGAAGCTGACAGAAGAGGGAAAGTGTATGAT
  AAATACATGTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGCAACCCGC
  AAGGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCAAACTGCTATGCAAAAGTT
  ATGATGGTTAACGGTGATCACAGGATAGGTATTTTTGCCAAGAGAGCCATCCAGACTGGC
  GAAGAGCTGTTTTTTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTCGGCATC
  GAAAGAGAAATGGAAATCCCTTGA
  
  Clone variation with respect to NM_152998.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152998 unedited
  CCCCGGGTGCGGATTTATGTATACGATCTCACTATAGGGCGGCCGCGAATTCGCACGAGG
  GTCCGACACCCGGTGGGACTCATAAGGCAGTGGAGCCCCGGCGGCGGCGGCGGCGGCGCG
  CGGGGGCGACGCGCGGGAACAACGCGAGTCGGCGCGCGGGACGAAGAATAATCATGGGCC
  AGACTGGGAAGAAATCTGAGAAGGGACCAGTTTGTTGGCGGAAGCGTGTAAAATCAGAGT
  ACATGCGACTGAGACAGCTCAAGAGGTTCAGACGAGCTGATGAAGTAAAGAGTATGTTTA
  GTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAATCTTAAACCAAGAATGGAAACAGC
  GAAGGATACAGCCTGTGCACATCCTGACTTCTGTGAGCTCATTGCGCGGGACTAGGGAGG
  TGGAAGATGAAACTGTTTTACATAACATTCCTTATATGGGAGATGAAGTTTTAGATCAGG
  ATGGTACTTTCATTGAAGAACTAATAAAAAATTATGATGGGAAAGTACACGGGGATAGAG
  AATGTGGGTTTATAAATGATGAAATTTTTGTGGAGTTGGTGAATGCCCTTGGTCAATATA
  ATGATGATGACGATGATGATGATGGAGACGATCCTGAAGAAAGAGAAGAAAAGCAGAAAG
  ATCTGGAGGATCACCGAGATGATAAAGAAAGCCGCCCACCTCGGAAATTTCCTTCTGATA
  AAATTTTTGAAGCCATTTCCTCAATGGTTCCAGATNAGGGCACAGCAGAAGAACTAAAGG
  GAAAATATAAAGAACTCACCCGACAGCAGCTCCCAGGCGCACTTNCTTNNCTGATGTACC
  CCACATAGATGGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTNCTNTCA
  TACGCTTTTCTGTAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152998 unedited
  NGGACTCGGGATGGCACTCCCCGTTCCAGGATAGCACTGAGGCAGGGTCACAGGGCTGCC
  ACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTATAGTCGAGTTTTTT
  TTTTTTTTTTTTGACAAGTTCAAGTTTTCTTTATTCAAAGTTGAAAAATGTACCATACTG
  CATTATTGCAAAAATTCACTGGTACAAAACACTTTGCTTCTGGTGAGAAGGCAATAAAAA
  GTTGATTTTTAAACTCATTACTATAAATTATTCTTACAGTACTTTGCAAATTCAGAATTT
  CAAACTGCATGTTCTTTTTCTAAATTGCCCACAGTACTCGAGGTTCCTGAAGCTAAGGCA
  GCTGTTTCAGAGGAGGGGGGAAGAGGTAGCAGATGTCAAGGGATTTCCATTTCTCTTTCG
  ATGCCGACATACTTCAGGGCATCAGCCTGGCTGTATCTGTAATCAAAAAACAGCTCTTCG
  CCAGTCTGGATGGCTCTCTTGGCAAAAATACCTATCCTGTGATCACCGTTAACCATCATA
  ACTTTTGCATAGCAGTTTGGATTTACCGAATGATTTGCAAAACGAATTTTGTTACCCTTG
  CGGGTTGCATCCACCACAAAATCATTGTTCAAGTTGAACAGAAAGCTGCACATGTATTTA
  TCATACACTTTCCCTCTTCTGTCAGCTTCATCTTGAGAAATAATCTCTCCACAGTATTCT
  GAGATGAATTCATTTTTCTGCACAGGATCTTTGATAAAAATCCCCCAGCCTGCCACGTCA
  GATGGTGCCCAGCATATATGCTTTTTGGAGCCCACGCTGATACTGCAGTTCTTGCAGGAA
  CATTTTTACTGTCCCAATGGTCAGCGGCTTCACAGTAAGACAGAGGTCAGGGTCACACTC
  TCGGACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152998
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152998.1, NP_694543.1
• RefSeq Size: 2563 bp
• RefSeq ORF: 2124 bp
• Locus ID: 2146
• UniProt ID: Q15910
• Cytogenetics: 7q36.1
• Domains: myb_DNA-binding, SET, DnaJ
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (2) lacks an in-frame exon and an in-frame segment in the coding region, as compared to variant 1. The resulting isoform (b) lacks two internal segments, as compared to isoform a.