IL4I1 (NM_152899) Human Untagged Clone

SKU SC101062

IL4I1 (untagged)-Human interleukin 4 induced 1 (IL4I1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: IL4I1
• Synonyms: FIG1; hIL4I1; LAAO; LAO
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101062 sequence for NM_152899 edited (data generated by NextGen Sequencing); ATGGCCCCATTGGCCCTGCACCTCCTCGTCCTCGTCCCCATCCTCCTCAGCCTGGTGGCC; TCCCAGGACTGGAAGGCTGAACGCAGCCAAGACCCCTTCGAGAAATGCATGCAGGATCCT; GACTATGAGCAGCTGCTCAAGGTGGTGACCTGGGGGCTCAATCGGACCCTGAAGCCCCAG; AGGGTGATTGTGGTTGGCGCTGGTGTGGCCGGGCTGGTGGCCGCCAAGGTGCTCAGCGAT; GCTGGACACAAGGTCACCATCCTGGAGGCAGATAACAGGATCGGGGGCCGCATCTTCACC; TACCGGGACCAGAACACGGGCTGGATTGGGGAGCTGGGAGCCATGCGCATGCCCAGCTCT; CACAGGATCCTCCACAAGCTCTGCCAGGGCCTGGGGCTCAACCTGACCAAGTTCACCCAG; TACGACAAGAACACGTGGACGGAGGTGCACGAAGTGAAGCTGCGCAACTATGTGGTGGAG; AAGGTGCCCGAGAAGCTGGGCTACGCCTTGCGTCCCCAGGAAAAGGGCCACTCGCCCGAA; GACATCTACCAGATGGCTCTCAACCAGGCCCTCAAAGACCTCAAGGCACTGGGCTGCAGA; AAGGCGATGAAGAAGTTTGAAAGGCACACGCTCTTGGAATATCTTCTCGGGGAGGGGAAC; CTGAGCCGGCCGGCCGTGCAGCTTCTGGGAGACGTGATGTCCGAGGATGGCTTCTTCTAT; CTCAGCTTCGCCGAGGCCCTCCGGGCCCACAGCTGCCTCAGCGACAGACTCCAGTACAGC; CGCATCGTGGGTGGCTGGGACCTGCTGCCGCGCGCGCTGCTGAGCTCGCTGTCCGGGCTT; GTGCTGTTGAACGCGCCCGTGGTGGCGATGACCCAGGGACCGCACGATGTGCACGTGCAG; ATCGAGACCTCTCCCCCGGCGCGGAATCTGAAGGTGCTGAAGGCCGACGTGGTGCTGCTG; ACGGCGAGCGGACCGGCGGTGAAGCGCATCACCTTCTCGCCGCCGCTGCCCCGCCACATG; CAGGAGGCGCTGCGGAGGCTGCACTACGTGCCGGCCACCAAGGTGTTCCTAAGCTTCCGC; AGGCCCTTCTGGCGCGAGGAGCACATTGAAGGCGGCCACTCAAACACCGATCGCCCGTCG; CGCATGATTTTCTACCCGCCGCCGCGCGAGGGCGCGCTGCTGCTGGCCTCGTACACGTGG; TCGGACGCGGCGGCAGCGTTCGCCGGCTTGAGCCGGGAAGAGGCGTTGCGCTTGGCGCTC; GACGACGTGGCGGCATTGCACGGGCCTGTCGTGCGCCAGCTCTGGGACGGCACCGGCGTC; GTCAAGCGTTGGGCGGAGGACCAGCACAGCCAGGGTGGCTTTGTGGTACAGCCGCCGGCG; CTCTGGCAAACCGAAAAGGATGACTGGACGGTCCCTTATGGCCGCATCTACTTTGCCGGC; GAGCACACCGCCTACCCGCACGGCTGGGTGGAGACGGCGGTCAAGTCGGCGCTGCGCGCC; GCCATCAAGATCAACAGCCGGAAGGGGCCTGCATCGGACACGGCCAGCCCCGAGGGGCAC; GCATCTGACATGGAGGGGCAGGGGCATGTGCATGGGGTGGCCAGCAGCCCCTCGCATGAC; CTGGCAAAGGAAGAAGGCAGCCACCCTCCAGTCCAAGGCCAGTTATCTCTCCAAAACACG; ACCCACACGAGGACCTCGCATTAA; ; Clone variation with respect to NM_152899.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152899 unedited; GATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACCAGCCGCGCTGTCCT; GCTGTCACCAAGAGCTGGAGACACCATCTCCCACCGAGAGTCATGGCCCCATTGGCCCTG; CACCTCCTCGTCCTCGTCCCCATCCTCCTCAGCCTGGTGGCCTCCCAGGACTGGAAGGCT; GAACGCAGCCAAGACCCCTTCGAGAAATGCATGCAGGATCCTGACTATGAGCAGCTGCTC; AAGGTGGTGACCTGGGGGCTCAATCGGACCCTGAAGCCCCAGAGGGTGATTGTGGTTGGC; GCTGGTGTGGCCGGGCTGGTGGCCGCCAAGGTGCTCAGCGATGCTGGACACAAGGTCACC; ATCCTGGAGGCAGATAACAGGATCGGGGGCCGCATCTTCACCTACCGGGACCAGAACACG; GGCTGGATTGGGGAGCTGGGAGCCATGCGCATGCCCAGCTCTCACAGGATCCTCCACAAG; CTCTGCCAGGGCCTGGGGCTCAACCTGACCAAGTTCACCCAGTACGACAAGAACACGTGG; ACGGAGGTGCACGAAGTGAAGCTGCGCAACTATGTGGTGGAGAAGGTGCCCGAGAAGCTG; GGCTACGCCTTGCGTCCNCAGGAAAAGGGCCACTCGCCCCGAGACATCTACCAGATGGCT; CTCAACCAGGCCCTCANAGACCTCAAGGCACTGGGCTGCAGAAAGGCGATGAAGAAGTTT; GAAAGGCACACGCTCTTGGAATATCTTCTCGGGGAGGGGAACCNNTGAGCCGCCGCCGTG; CAGCTTCTGGGAGACGTGATGTCCCGAGATGGCTTCNTCTATCTCAGCTTCGCCGAGGCC; CTTCGGGNCCACAGCTGCCTCAGCGACAGACTCCAGTACAGCCGCATCGTGGGTGGCTGG; GACCTGCTGCCGCGCGCGCTGCTGAGCTCGCTGTCCGGGCTTGTGCTGGTGGACGCGCCC; CGTGTGGCGATGACCCAGGCCNGNACGATGTGCACGTGCAGATCGAGACTCTN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152899 unedited; NNNAAATTCACTTGNACCGCGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAAAAAACTTTAATGCGAGGTCCT; CGTGTGGGTCGGGTTTTGGAAAAATAACTGGCCTTGGACTGGAGGGGGGCTGCCTTCTTC; CTTTGCCAGGTCATGCGAGGGGCTGCTGGCCACCCCATGCACATGCCCCTGCCCCTCCAT; GTCAAATGCCTGCCCCTCGGGGCTGGCCGTGTCCAATGCAGGCCCCTTCCGGCTGTTGAT; CTTGATGGGGGCCCCCAACGCCAACTTGACCGCCGTTTCCACCCAACCGTGCGGGTAGGC; GGTGTGCTCCCCGGCAAAATAAATGCGGCCATAAGGGACCGCCCAGTCATCCTTTTCGGT; TTGCCAAAACCCCGGCGGCTGTACCACAAAGCCACCCTGGCTGTGCTGGTCCTCCGCCCA; ACGCTTGACAACCCCGGTGCCGTCCCAAAGCTGGCCCACGACAGGCCCGTGCAATGCCCC; CACGTCGTCGAGCGCCAAGCGCAACGCCTTTTCCCGGCTAAAGCCGGCAAACGCTGCCGC; CGCGTCCGACCACGTGTACAAGGCCAGCAGCAGCGCGCCCTCGCGCGGCGGCGGGTAAAA; AATATGCCCCACGGGCGATCGGTGTTTGAGTGGCCCCCTTCAATGTGCTCCTCGCGCCAA; AAGGGCCTGCGGAACCCTAAGGACCCCTTGGTGGGCCGCACCTAATGAAACCTCCGCAAC; CCCTCCTCATGTTGGCGGGCCACCGCGCCAAAAAGTAAGCCCTTTACCGCGGTCCCTTCG; CTTTAGAAACCCAGTTGGGCCTTAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_152899
• Insert Size: 1875 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152899.1, NP_690863.1
• RefSeq Size: 1798 bp
• RefSeq ORF: 1704 bp
• Locus ID: 259307
• UniProt ID: Q96RQ9
• Cytogenetics: 19q13.33
• Domains: Amino_oxidase
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Cysteine and methionine metabolism, leucine and isoleucine degradation, Metabolic pathways, Phenylalanine, Phenylalanine metabolism, Tryptophan metabolism, tyrosine and tryptophan biosynthesis, Tyrosine metabolism, Valine
• Summary: This gene encodes a secreted L-amino acid oxidase protein which primarily catabolizes L-phenylalanine and, to a lesser extent, L-arginine. The expression of this gene is induced by the cytokine interleukin 4 in B cells. This gene is also expressed in macrophages and dendritic cells. This protein may play a role immune system escape as it is expressed in tumor-associated macrophages and suppresses T-cell responses. This protein also contains domains thought to be involved in the binding of flavin adenine dinucleotide (FAD) cofactor. Multiple transcript variants encoding different isoforms have been found for this gene. Some transcripts of this gene share a promoter and exons of the 5' UTR with the overlapping NUP62 gene. [provided by RefSeq, Jul 2020]; Transcript Variant: This variant (1) differs in the 5' UTR and coding region compared to variant 2. The resulting isoform (1) contains a distinct and shorter N-terminus, as compared to isoform 2. This transcript contains the first two exons of the upstream and is expressed from a different promoter.