MYZAP (NM_152451) Human Untagged Clone

SKU SC100915

MYZAP (untagged)-Human GRINL1A complex locus (GCOM1), transcript variant 13

Product Data

• Type: Human Untagged Clone
• Target Symbol: MYZAP
• Synonyms: GCOM1; Gup; MYOZAP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100915 sequence for NM_152451 edited (data generated by NextGen Sequencing); ATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGG; GCGCCCAGCAGGAGGGCAAATGTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCA; GTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAAT; GGAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGAT; CAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCACCAGTCAGCTGAAAGAAGAG; ATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGA; GACTATGATGAGATGAGACAGAAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCAT; GCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTT; AATGCCATGAACTCAGCCTTGGCGTCAGATTCCATTGGCCTGCAGAAAACCCTCGTGGAT; GTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAATCTGCAGCAGACGTATGAA; GCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAAC; CAGCTGCTAAAAATGAAGGTGGAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAG; ATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCAC; AGTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAA; GCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGAGAAAGACCAGAGGATCGGG; GAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTC; CTAGAACATGAAACAGAAATGTCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAG; CAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTG; CATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGAATAATGAACTACAAAGCAGG; TTGGACTATTTAACAGAAACCCAGGCCAAGACCGAAGTGGAAACCAGAGAGATAGGAGTG; GGCTGTGATCTTCTACCCAGCCAAACAGGCAGGACTCGTGAAATTGTGATGCCTTCTAGG; AACTACACCCCATACACAAGAGTCCTGGAGTTAACCATGAAGAAAACTCTGACTTAG; ; Clone variation with respect to NM_152451.6; 504 a=>g;525 a=>g;1173 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152451 unedited; NNNGGTCGCCATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACCAGGTCCCGC; GTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATCCTGCCCGGGAGGAACGCCGGCGTC; CAGCCCGCTACCGGCCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTC; TCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAATGTTTGCAGACTA; CGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGA; AAAGAGCAGCTTCTTGACCTGAGCAATGGAGAACCTACCAGGAAACTTCCTCAGGGTGTT; GTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGG; TGGTCCACCAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTG; GAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAGAAGATTCGACAG; CTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAA; ACCCAGTCGTCTGCCCTGGATCGTTTTAATGCCATGAACTCAGCCTTGGCGTCAGATTCC; ATTGGCCTGCAGAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAA; ATCAGAAATCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCANAGG; CAGTTGGAGGTAGCGCAAGTNGAAAACCAGCTGCTANAATGAAGGTGGNATCGTNCCAAG; AGCCCATGCTGAGTGATGCGAGAGATGACCANAAGCTGTACAGCCAGTATGAGGAGAGCT; GCAGGAAGACAGAGGAGCACAGTGCTGAGAAGGAGGCTCTTTA;
• Restriction Sites: NotI-NotI
• ACCN: NM_152451
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152451.1, NP_689664.1
• RefSeq Size: 2356 bp
• RefSeq ORF: 1401 bp
• Locus ID: 100820829
• UniProt ID: P0CAP1
• Cytogenetics: 15q21.3
• Summary: This gene encodes a protein that is abundantly expressed in cardiac tissue. The encoded protein localizes to intercalated discs in cardiomyocytes and functions as an activator of Rho-dependent serum-response factor signaling. Alternative splicing results in multiple transcript variants. Readthrough transcription also exists between this gene and the neighboring downstream gene POLR2M (polymerase (RNA) II (DNA directed) polypeptide M) and is represented with GeneID: 145781. [provided by RefSeq, Mar 2014]; Transcript Variant: This variant (2, also known as Gup2) lacks an in-frame exon in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1.